Assignment Flashcards
What is the aim of the investigation?
To investigate the effect of changing substrate concentration on enzyme activity
What are enzymes?
Biological catalysts that speed up chemical reactions and remain unchanged
What is an active site?
The region on an enzyme that allows it to bind to a specific substrate
What does ‘induced fit’ mean?
The active site alters its shape to fit the substrate better after binding
What are anabolic reactions?
Reactions that build small molecules into larger molecules using enzymes, requiring energy
What are catabolic reactions?
Reactions that break down larger molecules into smaller molecules using enzymes, releasing energy
What does metabolic pathways refer to?
Series of chemical reactions in a cell that are catalyzed by enzymes
What is affinity in the context of enzymes?
How one biological molecule is attracted to another molecule
What happens to the activation energy when a substrate binds to an enzyme?
The activation energy of the reaction is lowered
What occurs when all active sites of an enzyme are occupied?
The enzyme becomes saturated
What does saturation mean in enzyme activity?
The maximum amount of substrate present with all active sites occupied
What happens to the reaction rate when substrate concentration is too low?
The enzyme will work slowly and eventually stop working
Fill in the blank: Enzymes can have a _______ or low affinity.
high
True or False: Enzymes are involved in both anabolic and catabolic reactions.
True
What is the effect of increasing substrate concentration on enzyme reactions?
The enzyme reaction increases until all active sites are occupied
What is the consequence of adding more substrate when the enzyme is saturated?
Makes no difference to the reaction rate
