AST (GOT) FS* with/without Pyridoxal-5-Phosphate FS (P-5-P)

Question 1

AST (ASAT) stands for?

Answer 1

Aspartate Aminotransferase

Question 2

Intended Use

Answer 2

Diagnostic reagent for quantitative in vitro determination of ASAT
(GOT) in human serum or heparin plasma on automated
photometric systems.

Question 3

Alanine Aminotransferase (ALAT/ALT), formerly called called?

Answer 3

Glutamic Pyruvic Transaminase (GPT)

Question 4

Are the most important representatives of a group of
enzymes

Answer 4

AST and ALT

🩵Aspartate Aminotransferase
🩵Alanine Aminotransferase

….the aminotransferases or transaminases, which catalyze
the conversion of α-keto acids into amino acids by transfer of
amino groups.

Question 5

A liver specific enzyme?

Answer 5

ALT…. because it is primarily found in liver cells.

Question 6

Enzyme increased in connection with damages of heart or
skeletal muscle

Answer 6

AST

Question 7

Note:

Parallel measurement of ALAT and ASAT is, therefore, applied to
distinguish liver from heart or skeletal muscle damages.

Answer 7

🩵

Question 8

is used for differential diagnosis in liver diseases

Answer 8

ASAT/ALAT ratio

Question 9

Note:

While ratios ✅ <1 indicate MILD liver damage,

ratios ✅ >1 are
associated with SEVERE, often chronic liver diseases

Answer 9

🩵

Question 10

Method used in AST determination

Answer 10

Optimized UV-test according to IFCC

Question 11

what does IFCC stands for?

Answer 11

International Federation of
Clinical Chemistry and Laboratory Medicine

Question 12

Addition of _______, recommended by IFCC, stabilizes the activity of transaminases and avoids falsely low values in samples containing insufficient endogenous P-5-P………..

e.g.
from patients with myocardial infarction, liver disease and intensive
care patients

Answer 12

Pyridoxal-5-phosphate (P-5-P)

Question 13

Reagents in R1

Answer 13

✅TRIS…. (pH 7.65)…. 100 mmol/L
✅L-Aspartate 320 …..mmol/L
✅MDH (malate dehydrogenase) ………..≥ 800 U/L

Question 14

Reagents in R2

Answer 14

✅2-Oxoglutarate……..85 mmol/L
✅NADH………………….1 mmol/L

Question 15

Reagents storage and Stability….they are stable up to the date of expiry indicated on the kid….if stored at ????

Answer 15

2 – 8°C a

Question 16

The reagents contain ______ as a preservative….

Answer 16

Sodium azide (0.95 g/L)

……therefore DO NOT SWALLOW and AVOID contact with skin and mucous membranes…..

Question 17

Note: ….

In very rare cases, samples of patients with GAMMOPATHY….
might give falsified results

Answer 17

🩵

Question 18

(P-5-P) stands for??

Answer 18

Pyridoxal-5-Phosphate FS

Question 19

Reagent Preparation…

For the determination with P-5-P mix 1 part of P-5-P with _____parts
of reagent 1,

Answer 19

100 parts of Reagent 1

Question 20

✅Specimen used??
&
✅Stability???

Answer 20

🩵Human serum or heparin plasma

4 days………………..20 – 25°C
7 days………………….4 – 8°C
3 months………….–20°C

Note: Only freeze once. Discard contaminated specimens.

Question 21

Assay Procedure…..

✅ Wavelength
✅ Temperature
✅ Sample/Calibrator
✅ Reagent 1
✅ Reagent 2

Answer 21

Wavelength ……………..340/410 nm
Temperature ………………….37°C
Sample/Calibrator………..6.0 µL
Reagent 1……………………80 µL
Reagent 2…………………..20 µL

Question 22

Conversion Factor??

Answer 22

ASAT [U/L] x 0.0167 = ASAT [µkat/L]

NOTE:
🩵U/L………..Units Per Liter
🩵µkat/L…….Micro Katal Per Liter

Question 23

with P-5-P…..

✅ Measuring range up to____U/L ???

✅ Limit of detection ____ U/L

Answer 23

🩵600 U/L
🩵1.2 U/L

📚Note:
When values exceed this range, samples should be diluted
1 + 9 with NaCl solution (9 g/L) and the result multiplied by 10.

Question 24

Interfering substances (with P-5-P)….. Interferences ….≤ 10% up to

Answer 24

✅ Ascorbic acid……….30 mg/dL

✅ Bilirubin (conjugated and unconjugated) ……60 mg/dL

✅ Hemoglobin ……….100 mg/dL
✅ Lipemia (triglycerides) …200 mg/dL

📚Note that both with and without P-5-P has the SAME interfering substances and values….

Question 25

Reference Range…

✅With P-5-P
✅Without P-5-P

Answer 25

🐈 With P-5-P
>Women ……. < 31 U/L
>Men…………..< 35 U/L

🐈 Without P-5-P
>Same ras with