AUTOMATION

Question 1

BASIC COMPONENTS OF MOST HEMATOLOGY ANALYZERS

Answer 1

1. HYDRAULIC
2. PNEUMATICS
3. ELECTRICAL SYSTEM

Question 2

HYDRAULICS

Answer 2

Aspirating Unit
Dispensers
Dilutors
Hemoglobinometer
Aperture baths & flow cells
Mixing chambers

Question 3

PNEUMATICS

Answer 3

Vacuums & pressure for operating valves ang moving the sample through the system

Question 4

ELECTRICAL SYSTEM

Answer 4

Electronic analyzer and computing circuitry for processing data

Question 5

Classical methods of counting cells

Answer 5

Electrical Impedance

Optical Scatter

Question 6

ELECTRICAL IMPEDANCE is also known as

Answer 6

Electronic Resistance or Low-voltage Direct Current

Coulter Principle

Question 7

A modification sometimes used in conjunction with DC electrical impedance

Answer 7

RADIOFREQUENCY (RF)

Question 8

RADIOFREQUENCY is also known as

Answer 8

ALTERNATING CURRENT (AC) RESISTANCE

Question 9

What does RF measure?

Answer 9

CONDUCTIVITY

Question 10

TYPES OF ERRORS

Answer 10

Instrumental error

Error due to the nature of the spx

Question 11

Most common problem in cell counting

Answer 11

APERTURE PLUGS

Question 12

Causes of POSITIVE ERROR

Answer 12

Bubbles
Extraneous electrical pulse
Aperture plugs
Improper setting of aperture current or threshold

Question 13

Causes of NEGATIVE ERRORS

Answer 13

Excessive lysing of RBC

Improper setting of aperture current or threshold

Question 14

ERRORS DUE TO THE NATIRE OF THE SPX

Answer 14

1. GIANT PLATELET
2. FRAGMENTS PF WBC CYTOPLASM
3. SOME ABN. RBC w/c RESIST LYSIS

Question 15

Define CELL COINCIDENCE

Answer 15

CHANCE OF ONE CELL BEING COUNTED MORE THAN ONCE

Question 16

UTILIZED TO REDUCE CELL COINCIDENCE

Answer 16

HYDRODYNAMIC FOCUSING

Question 17

USED TO SEPARATE CELL POPULATIONS AND SUBPOPULATIONS

Answer 17

THRESHOLD

Question 18

OPTICAL SCATTER =

Answer 18

FLOW CYTOMETER

Question 19

OPTICAL SCATTER: Light sources

Answer 19

Tungsten-halogen lamp

Helium-neon laser

Question 20

LASER LIGHTS: Avantagws

Answer 20

Intensity
Stability
Monochromatism

Question 21

THREE INDEPENDENT PROCESSES IN OPTICAL SCATTER

Answer 21

Diffraction
Refraction
Reflection

Question 22

Bending of light around corners using SMALL angles

Answer 22

DIFFRACTION

Question 23

Bending of light because of CHANGE un SPEED using INTERMEDIATE angles

Answer 23

REFRACTION

Question 24

Light rays turned back by obstruction using large angles

Answer 24

REFLECTION

Question 25

FORWARD ANGLE (FORWARD LOW)

Answer 25

CELL SIZE OR VOLUME

Question 26

SIDE ANGLE (RIGHT HIGH)

Answer 26

GRANULARITY OR LOBULARITY