B1 - Cell Structure And Tranport Flashcards
What are the basic units of measurement related to microscopy?
1 kilometre (km) = 1000 metres (m), 1 m = 100 centimetres (cm), 1 cm = 10 millimetres (mm), 1 mm = 1000 micrometres (µm), 1 µm = 1000 nanometres (nm)
A nanometre is 0.000 000 001 metres (or written in standard form as 1 × 10^-9 m)
What is the maximum magnification of a light microscope?
Around 2000 times (x2000)
School microscopes usually only magnify several hundred times.
When were the first light microscopes developed?
In the mid-17th century
What is the primary function of a light microscope?
To use a beam of light to form an image of an object
What are the types of electron microscopes and their features?
Transmission electron microscopes (2D images, high magnification and resolution), Scanning electron microscopes (3D images, lower magnifications)
Electron microscopes can magnify objects up to around 2000000 times.
What is a key disadvantage of electron microscopes?
They are large, very expensive, and require special controlled environments
How do you calculate the overall magnification of a microscope?
Multiply the magnification of the eyepiece lens by the magnification of the objective lens
If the eyepiece lens is x4 and the objective lens is x10, what is the overall magnification?
x40
What is the formula to calculate the size of an object under a microscope?
Size of image = magnification × size of real object
What does resolution in microscopy refer to?
The ability to distinguish between two objects that are close together
What is the resolving power of the best light microscopes?
About 200 nm
What is the significance of the magnification triangle in microscopy?
It helps to calculate the size of an object based on the image size and magnification
True or False: Light microscopes can only view dead specimens.
False
Light microscopes can magnify live specimens.
Fill in the blank: The first light microscopes were developed in the ______ century.
17th
What is the resolution between a light microscope and an electron microscope?
The resolution of an electron microscope is significantly higher than that of a light microscope.
How do you calculate the magnification of a specimen?
Magnification can be calculated using the formula Icm = 10n.
What is the equivalent of 1 mm in micrometers?
1 mm = 10 micrometers.
What is the equivalent of 1 picometer in meters?
1 pm = 10^-12 meters.
What parts are included in a light microscope?
- Eyepiece
- Coarse focus
- Objective lens
- Stage
- Fine focus
- Slide
- Light
True or False: A light microscope can be used to observe live specimens.
True
Fill in the blank: The formula to calculate real size is _______.
Icm = 10n
What is the purpose of the coarse focus on a microscope?
To bring the specimen into general focus.
What is the function of the objective lens in a microscope?
To magnify the specimen.
Fill in the blank: The _______ holds the slide in place on the stage.
stage clips