B2: Organisation Flashcards
(100 cards)
What is a tissue?
A group of similar cells that work together to carry out a particular function.
What is a organ?
A group of similar tissues that work together to carry out a particular function.
What is a organ system?
A group of similar organs that work together to carry out a particular function.
What is a organism?
Several groups of organ systems that form an individual animal, plant, or single celled organism.
What is an enzyme?
A substance produced by a living organism which acts as a catalyst to bring about a specific reaction.
How do enzymes work?
- the substrate collides with the enzyme and becomes attached.
- the enzyme catalyses breakdown of substrate
- the enzyme is unchanged and can be used again
What factors effects the rate of a reaction?
Temperature, pH, enzyme concentration, substrate concentration, surface area, pressure.
What is meant by the term optimum?
The temperature and pH value where an enzyme works best.
What is meant by an enzyme denaturing?
An enzyme being destroyed by high temperatures and pH(too acid or too alkali)
What happens to the active site when an enzyme denatures?
The active site changes so the substrate can no longer fit in it.
What is the lock and key theory?
The unique fit of the enzyme with the substrate.
What is the relationship between the active site and substrate?
They are complementary to each other.
How do you investigate the rate of enzyme activity?
- place a drop of iodine starch indicator on to each spot of the spotting tile.
- measure 3cm3 of starch using a syringe and dispense into a boiling tube and put pH5 buffer and amylase into another small test tube.
- set up a water bath with 150ml of cold water and add boiling water until it reaches 35-37*C
- place the test tubes in the water bath and when they reach 37*C add the test tube into the boiling tube and start the stopwatch immediately.
- after 15s, using a clean pipette remove one drop from the starch and amylase mix and drop this onto the first spot of the spotting tile.
- repeat this until there is no colour change.
- repeat these steps using pH6, pH7, and pH8
What is digestion?
The breakdown of large insoluble food molecules into small, soluble food molecules.
What are the two types of digestion?
Mechanical and chemical
What is mechanical digestion and what are two examples of it?
Breaking down large pieces of food through physical processes such as chewing and squeezing of the stomach.
What is chemical digestion and what are two examples of it?
Breaking down large pieces of food through the use of digestive enzymes.
Why do large food molecules need to be broken down?
To be absorbed into the bloodstream.
What do the liver and gallbladder do in digestion?
Bile is made in the liver but stored in the gallbladder and improves fat digestion. It does this because it’s an alkali meaning it neutralises stomach acid and it emulsifies fat so it’s easier for lipase to breakdown.
What do carbohydrates do?
Provide energy.
What do proteins do?
For growth and repair.
What do lipids do?
To provide energy. Also to store energy in the body and insulate it against the cold.
How do you prepare for food tests?
- Grind up the food and transfer it to a small beaker and add distilled water
- stir the mixture so that some of the food dissolves in the water
- fold the piece of filter paper and place that in the funnel
- pour the mixture form the beaker through the filter paper so you collect about a 1cm depth watery liquid
How do you test for simple carbohydrates (sugars)?
- add 10 drops of benedict’s solution to the solution into one of the test tubes with the filtrate in
- put hot water from the kettle in a large beaker. the water should not be boiling and put the test tube in for around 5 minutes
- a positive test goes from blue to brick red/ cloudy orange/ yellow/ green (depends on concentration)