B21 - Recombinant DNA technology Flashcards
1
Q
What does PCR stand for?
A
Polymerase chain reaction
2
Q
What five things need to be present for the PCR process to take place?
A
- The DNA fragment
- DNA polymerase
- Primers
- Nucleotides
- Thermocycler
3
Q
Give an example of DNA polymerase
A
Taq polymerase: obtained from bacteria in hot springs, and is therefore tolerant to heat - won’t denature.
4
Q
What are primers?
A
Short sequences of nucleotides
5
Q
What is a thermocycler?
A
A computer controlled machine that varies temperature over a period of time.
6
Q
Outline the three stages of the polymerase chain reaction.
A
- Separation of the DNA strands
- Addition [annealing] of the primers
- Synthesis of DNA
7
Q
What is an advantage of in vitro gene cloning?
A
Extremely rapid, and it does not require living cells.
8
Q
What is the first stage in recombinant DNA technology?
A
Isolate the fragments of DNA
9
Q
A
