Bacteria

Question 1

What do you see on a gram film for staphylococci?

Answer 1

Gram positive cocci in clusters

Question 2

What do you see on a gram film for Neisseria?

Answer 2

Gram negative cocci

Question 3

What do you see on a gram film for Bacillus.anthrancis?

Answer 3

Gram positive bacilli

Question 4

What do you see on a gram film for E.coli?

Answer 4

Gram negative bascilli

Question 5

What do you see on a gram film for streptococci?

Answer 5

Gram positive cocci in chains

Question 6

What do you see on a gram film for Moraxella?

Answer 6

Gram negative cocci

Question 7

What do you see on a gram film for listeria monocytogenes?

Answer 7

Gram positive bacilli

Question 8

What do you see on a gram film for campylobacter?

Answer 8

Gram negative bacilli

Question 9

What do you see on a gram film for enterococci?

Answer 9

Gram positive cocci

Question 10

What do you see on a gram film for veilonella spp

Answer 10

Gram negative cocci

Question 11

What do you see on a gram film for Corynebacteria.diptheriae?

Answer 11

Gram positive bacilli

Question 12

What do you see on a gram film for pseudomonas?

Answer 12

Gram negative bacilli

Question 13

What do you see on a gram film for salmonella?

Answer 13

Gram negative bacilli

Question 14

What do you see on a gram film for shigella?

Answer 14

Gram negative bacilli

Question 15

What do you see on a gram film for proteus?

Answer 15

Gram negative bacilli

Question 16

What organism does gram positive cocci in clusters suggest?

Answer 16

Staphylococcus

Question 17

What organism does gram positive cocci in chains suggest?

Answer 17

Streptococcus

Question 18

What type of organism would I use a coagulase test on and what would this show?

Answer 18

Staphylococcus

positive = staph.aureus
negative = staph.epidermidis or staph.saprophiticus

Question 19

What type of organism would I use a haemolysis test on and what would this show

Answer 19

Streptococcus

Partial = alpha haemolysis
Complete = beta haemolysis
None = non-haemolytic

Question 20

What type of organism would you do Lancefield group testing for and what would this show

Answer 20

Test = beta haemolytic streptococcus AND non-haemolytic

Group A = S.pyogenes
Group B = S.agalactiae
Group G = S.dysgalactiae

non-haemolytic Group D = S.bovis or enterococcus spp.

Question 21

Give an example of a group A beta haemolytic streptococcus?

Answer 21

S.pyogenes

Question 22

Give an example of a group B beta haemolytic streptococcus?

Answer 22

S.agalactiae

Question 23

Give an example of a group G beta haemolytic streptococcus?

Answer 23

S.dysgalactiae

Question 24

Give 2 examples of group D non-haemolytic streptococcus?

Answer 24

S.bovis

Enterococcus spp.

Question 25

Give an example of a coagulase positive staphylococcus?

Answer 25

Staphylococcus aureus

Question 26

Give 2 examples of coagulase negative staphylococcus?

Answer 26

Staph.epidermidis | Staphy.saprophiticus

Question 27

On what would you use an optocin test and what would this show?

Answer 27

Test = alpha haemolytic streptococcus ``` Sensitive = s.pneumoniae Resistant = s.viridans ```

Question 28

What test would you do on a gram negative bacillus and what would this show?

Answer 28

Test = MacConkey or CLED or XLD Lactose fermenting = E.coli, klebsiella Non-lactose fermenting = salmonella, shigella, pseudomonas, proteus

Question 29

On what type of organism would you use on oxidase test and what would this show?

Answer 29

Test = non-lactose fermenting gram negative bacilli ``` Positive = peudomonas Negative = proteus, shigella, salmonella ```

Question 30

Give 2 examples of lactose fermenting gram negative bacilli:

Answer 30

E.coli | Klebsiella

Question 31

Give 3 examples of oxidase negative non-lactose fermenting gram negative bacilli:

Answer 31

Salmonella Shigella Proteus

Question 32

Give 4 examples of non-lactose fermenting gram negative bacilli:

Answer 32

Salmonella Shigella Proteus Pseudomonas