Bacteria Identification Flashcards
(26 cards)
Supportive culture media
Allows growth of most organisms
Blood agar
Enrichment culture media
Nutrient specificity
Chocolate agar
Selective culture media
Supresses growth of unwanted bacteria
MacConkey agarD
Differential culture media
Metabolic characteristics
Wet mount
Put on glass slide
Gram staining
- smear on plate
- crystal violet
- iodine = set
- ethanol = decolonized, desolve outermost membrane (gram -)
- red saffron = counter stain
Acid fast staining
Acid fast positive - red
Acid fast negative - blue
High amounts of lipids
Microscopy
Darkfiled
Electron
Fluorescent
Colony morphology
Hemolysis
a - partial - greenish
b - complete - hole around colonies
gamma - none
Pigmentation
Change in color of plate
Pink MacConkey will switch to yellow with non-lactose fermenting bacteria, stay pink with lactose fermenters
Swarming
Motility
Catalase
Organism that produce catalase, important for breakdown of O2 byproducts
Carb utilization
Color change depending on pH
Coagulase
Clot blood
Oxidase
Presence of cytochrome oxidase enzyme - aerobic respiration
Indole
Degrade tryptophan to get indole
Immunological
ELISA - test antibodies
Automated systems
Mass spectrometry
Antimicrobial susceptibility testing
Guide for reactions with antibodies
MIC
Concentration that inhibits growth
MBC
Concentration that kills bacteria
Bacteriostatic
Inhibits growth
MBC > 4 X MIC
Bactericidal
Kills
MBC = MIC
Kirby Bower
Disk diffusion
Antibiotics put on film of bacteria, zone of inhibition = diameter of circle - determine if susceptible, resistant, intermediate
