Bacteria Identification Flashcards by Gurjinder Olekh

If a Bacteria does not stain Gram Positive/Negative , what are the possible classes of Organisms?

Obligate Intracellular

Spirochetes

Mycoplasmas (Mollicutes)

Acid Fast (Myobacteria)

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If Gram Positive Cocci Shaped Bacteria is found to be Catalase Positive , what species could it be and what would the next appropriate examination?

Any Staphylococci Bacterium ( Facultative Anaerobes)

Staphylococcus Aureus

Staphylococcus Epidermidis

Staphylococcus Saprophyticus

Next test would be a Coagulase Exam ( Fibrin Formation)

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If a Gram Positive Bacterium is Catalase + , and Coagulase + , what species has been identified?

Staphylococcus Aureus

Normal Flora on skin , in nares

Colonies often yellow

Beta Hemolytic

Salt Tolerant ( Mannitol Salt Agar)

Produce Acid From Mannitol ( Red to Yellow)

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If a Gram Positive Cocci is catalase + , and Coagulase Neg , and susceptible to Novobiocin, what are the possible species of bacterium?

Normal Flora on skin , nares and vagina

Non Hemolytic

S epidermidis

Novobiocin Suceptible

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If a Gram Positive Bacteria is:

Catalase Positive

Coagulase Negative

Cocci

Novobiocin Resistant

What is the species?

S saprophyticus

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What species of bacterium are Gram Positive and Catalase Negative?

Streptococci/Enterococci

Anaerobes

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If a bacterium is found to be:

Gram Positive Cocci

Catalase Negative

Alpha Hemolytic

Optochin Susceptible

What is the Identity?

Streptococcus Pneumoniae

Aerotolerant

Bile Soluble

Diplococci

No Lancefield Antigen Result

Normal Pharyngeal Flora

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If a Bacterium is found to be:

Gram Positive Cocci

Catalse Negative

Alpha Hemolytic

Optochin Resistant

Bile Resistant

What is the Identity of the Organism?

Viridans Streptococci

Aerotolerant

Normal Oral Flora

S mitis

Various Lancefield Groups

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If a Bacterium is found to be:

Gram Positive Cocci

Catalse Negative

Beta Hemolytic

Bacitracin Susceptible

What is the species?

Streptococcus Pyogens

Aerotolerant

PYR +

Lancefield Group A

Can be part of normal pharyngeal flora

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If a Bacterium is found to be:

Gram Positive Cocci

Catalse Negative

Beta Hemolytic

Bacitracin Resistant

What is the the Species?

Streptococcus Agalactiae

Aerotolerant

PYR -

Lancefield Group B

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If a Bacterium is found to be:

Gram Positive Cocci

Catalase Negative

Non Hemolytic (Y)

What is the species?

Enterococcus

Some Aerotolerant

Lancefield Group D

Black Colonies on Bile Esculin Agar

Esculin and Esculitin

Black Precipitate

*******SOME STREPTOCOCCUS WONT GROW ON OR WILL BE CLEAR OF ESCULIN AGAR********

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If a Bacterium is found to be:

Gram Positive Bacilli

Endospore Forming

Catalase Positive

What is the species?

Bacillus

Soil and Water

Aerobe

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If a Bacterium is found to be:

Gram Positive Bacilli

Endospore Forming

Catalase Negative

What species is present?

Clostridium

Found in soil , water , GI tract

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If a Bacterium is found to be:

Gram Positive Bacilli

Non Endospore Forming

Aerobe

Club Shaped

What Species is present?

Corynebacterium Diphtheriae

Normal Part of Skin, Vaginal , Pharyngeal flora

Toxigenic Strains cause disease

Chinese Letter appearance

Brown Black Colonies on Tellurite Agar

Loeffler Agar coloines are white , cells have polar metachromatic granules

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If a Bacterium is found to be:

Gram Positive Bacilli

Non Endospore Forming

Aerobes

Low Growth Temperatures as Low as 2 Degrees

What species is present?

Listeria

Tumbling Motility in Broth

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If a Bacterium is found to be:

Non Endospore Forming

Gram Positive Bacilli

Aerobes Partial Acid Fast

What Species is present?

Nocardia

Filamentous Branching

Found in Soil and Water

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If a Bacterium is Found to be:

Non Endospore Forming

Anaerobe

Gram Positive Bacilli

Acne

What Species is present?

Propionibacterium

Chains or clumps , short rods

Common on skin , vagina and oropharynx

Opportunistic Infection

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If a Bacterium is found to be:

Gram Positive Bacilli

Anaerobe

Catalase Neg

Strict or Aerotolerant

Endocarditis

What Species is present?

Lactobacillus

Part of Normal Flora

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If a Bacterium is found to be:

Gram Positive Bacilli

Non Endospore Forming

Anaerobe

Catalase Neg

Knot up into yellow flecks visible in pus

What Species is Present?

Study These Flashcards

Actinomyces

Filamentous Branching

Part of normal oral , GI Flora

Cervicofacial Infection Most Common

If a Bacterium is Found to be:

Gram Negative Cocci

Oxidizes Glucose

Oxidase Positive

Catalase Positive

Found on Choclate Agar

What Species is Present?

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Neisseria Gonorrhoeae

Diplococci

PMNs in direct smears

Aerobes but use CO2

Non Motile

Some Found in Throat

If a Bacterium is Found to be:

Gram Negative Cocci

Diplococci

Oxidize Glucose and Maltose

Some Species normal in Throat

What Species is Present?

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Neisseria Meningitidis

Infects Blood and CNS

Non Motile

Aerobes

Often Associates with PMNs

If a Bacterium is Found to be:

Gram Negative Bacilli

Coccobacilli/Pleomorph

Aerobe (Non Fermenter)

Oxidase Negative

Catalase Positive

What Species is Present?

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Acinetobacter

Cocci or Rods

Normal Oropharyngeal Flora

Soil/Plants

Easy to Grow on Blood Agar

If a Bacterium is found to be:

Gram Negative Bacilli

Coccobacilli/Pleomorph

Aerobes (Non Fermenter)

Oxidase and Catalase Pos

What Species is Present?

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Moraxella

Diplococcus

Normal Respiratory Flora

Blood Agar

If a Bacterium is Found to be:

Gram Negative Bacilli

Coccobacilli/Pleomorphs

Aerobes ( Non Fermenter)

Oxidase Neg

3 Day Incubation

What Species is Present?

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Francisella Sp.

Very Small , Faint Staining

BCYE or Choclate Agar + Cys

Serology Testing

If a Bacterium is found to be: Gram Negative Bacilli Coccobacilli/Pleomorphs Aerobes **(Non Fermenter)** Oxidase Pos What Species is Present?

***Brucella Sp.*** Small Intracellular Enriched Blood Agar 3 Days + Serology

If a Bacterium is found to be: Gram Negative Bacilli Coccobacilli/Pleomorphs Aerobes **(Non Fermenter)** Bordet Gengou , Regan Lowe Agar 4 to 12 dat incubation What Species is Present?

***Bordetella Sp.*** PCR often used Oxidase Variable , motility variable

If a Bacterium is found to be: Gram Negative Bacilli Coccobacilli/ Pleomorphs Facultative Anaerobe Requires Factor X and Factor V What Species is Present?

***Haemophilus*** Often Seen as a Rod , can grow as long as a filament Choclate Agar Oxidase Pos and Neg

If a Bacterium is found to be: Gram Negative Bacilli Coccobacilli/Pleomorph Facultative Anaerobes Bipolar Staining What Species is Present?

***Pasteurella*** Oxidase Positive

If a Bacterium is found to be: Gram Negative Bacilli Coccobacilli/Pleomorph Anaerobes Stain Poorly Can be motile What Species is Present

***Bacteroides*** Pleomorphic Easy to Grow Normal GI , Oral Flora

If a Bacterium is found to be: Gram Negative Bacilli Coccobacili/Pleomorph Anaerobes Normal Oral Flora What Species is Present?

***Prevotella*** Very Small Slow Growing Fastidious Black Colonies on Bap

If a Bacterium is found to be: Gram Negative Bacilli Coccobacilli/Pleomorphs Anaerobes Black Colonies of BAP What Species is Present?

***Porphyromonas*** Very Small Slow Growing Fastidious Normal Oral Flora

If a Bacterium is found to be: Gram Negative Bacilli Curved and Spiral Rods (Motile) Aerobe Gull Wing Grow at 42 Degrees What Species is Present?

***Campylobacter Sp.*** Oxidase Positive Motile MicroAerophilic

If a Bacterium is found to be: Gram Negative Bacilli Curved and Spiral Rods (Motile) Aerobes Oxidase and Urease Positive What Species is Present?

***Helicobacter Sp.*** Microaerophilic

If a Bacterium is found to be: Gram Negatice Bacilli Curved and Spiral Rods (Motile) Facultative Anaerobe Alkalophile Like Salt What Species is Present?

***Vibrio Sp.*** Comma Shaped Rods Oxidase Positive Motile

If a Bacterium is found to be: Gram Negative Bacilli Straight Rods Facultative Anaerobes Oxidase Negative Ferment Glucose What Species is Present?

***Enterobacteriaceae*** Normal GI Flora Medical Importance ***Serratia*** can be Lac +/- often produces red pigment

If a Bacterium is found to be: Gram Negative Bacilli Straight Rods Facultative Anaerobes Oxidase Negative Lactose Fermenting (CEEK) Urease and H2S (+/-) What Species is Present?

***Citrobacter*** Motile

If a Bacterium is found to be: Gram Negative Bacilli Straight Rods Facultative Anaerobes Oxidase Negative Lactose Fermenting (CEEK) Urease and H2S (+/-) What Species is Present?

***Enterobacter***

If a Bacterium is found to be: Gram Negative Bacilli Straight Rods Facultative Anaerobes Oxidase Negative Lactose Fermenting (CEEK) Urease and H2S Negative Often Encapsulated What Species is Present?

***Escherichia***

If a Bacterium is found to be: Gram Negative Bacilli Straight Rods Facultative Anaerobes Oxidase Negative Lactose Fermenting (CEEK) Urease(+) and H2S(-) Non Motile Often Highly Mucoid What Species is Present?

***Klebsiella***

If a Bacterium is found to be: Gram Negative Bacilli Straight Rods Facultative Anaerobes Oxidase (-) Lactose Non Fermenting (ShYPS) Non H2S Producing Urease Negative What Species is Present?

***Shigella*** Non motile

If a Bacterium is found to be: Gram Negative Bacilli Straight Rods Facultative Anaerobes Oxidase (-) Lactose Non Fermenting (ShYPS) Non H2S Producing Can grow at less that 4 Degrees What Species is Present?

***Yersinia*** Bipolar Staining Slower Growing Coagulase (+/-) Urease (+/-) Motility (+/-)

If a Bacterium is found to be: Gram Negative Bacilli Straight Rods Facultative Anaerobes Oxidase (-) Lactose Non Fermenting (ShYPS) H2S Producing Swarming Motility What Species is Present?

***Proteus*** Urease +

If a Bacterium is found to be: Gram Negative Bacilli Straight Rods Facultative Anaerobes Oxidase (-) Lactose Non Fermenting (ShYPS) H2S Producing Urease Negative What Species is Present?

***Salmonella*** Motile

If a Bacterium is found to be: Gram Negative Bacilli Straight Rods Aerobes Oxidase (+) Non Fermenters Resist Cetrimide Multi Drug Resistant What Species is Present?

***Pseudomonas Sp.*** Beige on MacConkey Produce Pyocyanin Can Grow Anaerobically with Alternate Electron Acceptors

If a Bacterium is found to be: Gram Negative Bacilli Straight Rods Aerobes Oxidase (+) Non Fermenters Silver Stain Direct Fluorescents Antibody (DFA) What Species is Present?

***Legionella Sp.*** Thin , short or long Gram Stain Poorly BCYE Agar + Cysteine , Iron 3-5 Days incubation

If a Bacterium is found to be: Gram Negative Bacilli Straight Rods Anaerobes Thin and Pointy What Species is Present?

***Fusobacterium***

Bacteria Identification Flashcards

(46 cards)