Bacterial biochemical tests and agars 2

Question 1

What are the organisms which are lactose non-fermenters and oxidase negative?

Answer 1

Pseudomonas luteola
Pseudomonas oryzihabitans
Some Burkholderia species*
Stenotrophomonas Maltophilia
Acinetobacter species
Roseomonas species
Pandoraea species

Question 2

what is the selective culture media for PSA?

Answer 2

Cetrimide and Naladixic Acid (CN)

Question 3

Lab ID of PSA?

Answer 3

1-Aerobic GNB
2-Non-spore-forming
3-Motile
4-Oxidase +ve
5-catalase +ve
6-grape-like smell of aminoacetophenone

Question 4

What is the Typing method thast used for Pseudomonas aeruginosa?

Answer 4

variable number tandem repeat (VNTR) analysis

Question 5

What is AMRHAI reference unit?

Answer 5

It is the Antimicrobial Resistance and Healthcare Associated Infections

The national reference unit for investigating antimicrobial resistance in healthcare-associated bacteria

Question 6

Acinitobacter spp characteristics?

Answer 6

GN coccobacilli (appearing as diplococci)
Strictly aerobic
Non-fermenting
Non-motile
Non-spore-forming

Catalase-positive
Oxidase-negative

Colonies are normally smooth, sometimes mucoid, usually non-pigmented.

Question 7

What are the spp that belong to Acinetobacter calcoaceticus-baumannii complex” (ACB complex)??

Answer 7

A. calcoaceticus
A. baumannii
A. pittii
A. nosocomial

Question 8

The two most frequently identified Acinetobacter species causing bacteraemia?

Answer 8

A. lwoffii (30%)
A. baumannii (19.5%)

Question 10

T/F:
A baumanii may not readily decolourise and appear as pleomorphic Gram-variable organisms

Answer 10

T