Bacteriological Analysis of Water

Question 1

are a group of closely related, mostly harmless bacteria that live in soil and water as well as the gut of animals.

Answer 1

coliforms

Question 2

is a hygienic indicator and high level of coliform counts generally indicates unsanitary condition or poor hygiene practices during or after food production

Answer 2

coliform count

Question 3

These are organisms present in the environment and in the feces of all warm-blooded animals and humans

Answer 3

coliform bacteria

Question 4

will not likely cause illness

Answer 4

coliform bacteria

Question 5

coliform bacteria is

gram positive/negative

Answer 5

gram negative

Question 6

oxygen requirement of coliform bacteria

Answer 6

facultative anaerobic

Question 7

shape of coliform bacteria

Answer 7

rod shape

Question 8

does coliform bacteria ferment lactose

Answer 8

yes

Question 9

coliform ferments lactose to produce what

Answer 9

acid and gas

Question 10

coliform bacteria ferments lactose to produce acid and gas within what variables

Answer 10

48 hours at 35oC

Question 11

used as an indicator of sanitary quality of water or as a general indicator of sanitary condition in the food-processing environment.

Answer 11

detection of coliforms

Question 12

three groups of coliform bacteria

Answer 12

total coliform
fecal coliform
E.coli (O157:H7)

Question 13

indicates environmental contaminaton

Answer 13

total coliform

Question 14

fecal coliform + e. coli =

Answer 14

fecal contamination

Question 15

Are commonly found in the environment (soil or vegetation) and are generally harmless.

Answer 15

total coliform bacteria

Question 16

If only __ ____ bacteria are detected in drinking water, the source is probably environmental

Answer 16

total coliform

Question 17

____ contamination is not likely if total coliform is detected

Answer 17

fecal

Question 18

Are a sub-group of total coliform bacteria.

Answer 18

fecal coliform bacteria

Question 19

They appear in great quantities in the intestines and feces of people and animals

Answer 19

fecal coliform bacteria

Question 20

its presence often indicates recent fecal contamination, meaning that there is a greater risk that pathogens are present than if only total coliform bacteria is detected

Answer 20

fecal coliform bacteria

Question 21

It is a sub-group of the fecal coliform group.

Answer 21

e. coli

Question 22

Most___ bacteria are harmless and are found in great quantities in the intestines of people and warm-blooded animals

Answer 22

e.. coli

Question 23

straincauses a severe intestinal infection in humans

Answer 23

O157:H7

Question 24

The presence of___ in a drinking water sample almost always indicates recent fecal contamination, meaning there is a greater risk that pathogens are present.

Answer 24

E. coli

Question 25

___or treating contaminated drinking water with a ___destroys all forms of E. coli, including O157:H7.

Answer 25

boiling disinfectant

Question 26

It is a method for estimating the number of bacteria in a food or water sample.

Answer 26

most probable number method

Question 27

In this technique, replicate portions of the original sample are cultured to determine the presence or absence of microorganisms in each portion.

Answer 27

most probable method

Question 28

three phases of MPN

Answer 28

presumptive confirmatory completed

Question 29

Almost all the methods used to detect E. coli

Answer 29

MPN

Question 30

are enumeration methods that are based on lactose fermentation

Answer 30

total coliform or fecal coliform

Question 31

It is used for detection and estimation of coliform in water sample.

Answer 31

presumptive test

Question 32

broth in presumptive test

Answer 32

lactose containing broth medium

Question 33

positive result of turbidity

Answer 33

turbidity of media and gas formation

Question 34

equipment used for presumptive test

Answer 34

Durham's tube

Question 35

MPN table will be used to estimate the most probable number of viable cell.

Answer 35

presumptive test

Question 36

Confirmed test is done to determine that the coliforms are of fecal origin or not.

Answer 36

confirmed test

Question 37

Also determine if the isolated bacteria is E. coli or not.

Answer 37

confirmed test

Question 38

are inoculated in Brilliant Green Lactose bile Broth (BGLBB) broth.

Answer 38

positive presumptive test

Question 39

broth for confirmed test

Answer 39

Brilliant Green Lactose Bile Broth (BGLBB)

Question 40

Positive BGLBB samples are inoculated in selective media like

Answer 40

eosin methylene blue agar

Question 41

indicates the presence of E. coli.

Answer 41

green metallic sheen colonies

Question 42

is performed on all samples showing a positive result in the confirmed test.

Answer 42

completed test

Question 43

is e coli spore forming

Answer 43

no

Question 44

The formation of gas in the fermentation tube and the presence of gram-negative, non-spore-forming, rod-shaped bacteria in the agar culture may be considered a satisfactorily ___ test

Answer 44

completed

Question 45

1 mpn = __ cfu = ___ viable cell

Answer 45

1 cfu 1 viable cell

Question 46

presuumptive test complete equipment

Answer 46

injective tube durham tube

Question 47

presumptive test kind of strength

Answer 47

5 double strength 10 single strength

Question 48

doube the concentration of lactose broth used to test for coliform organisms in water

Answer 48

double strength

Question 49

how many water sample is placed in double strenth

Answer 49

10 ml

Question 50

how many water ml is placed in SS tubes (both amount)

Answer 50

1 ml 0.1 ml

Question 51

to confirm strain of e. coli, it has to undergo

Answer 51

antigen-antibody test