Bacteriology Flashcards
(22 cards)
What bugs don’t gram stain well?
Treponema - dark filed microscopy and fluorescent antibody staining
Rickettsia (intracellular parasite)
Mycobacterium (high lipid content in cell wall, requires acid fast stain)
Mycoplasma (no cell wall)
Legionella (silver stain)
Chlamydia (intracellular, lacks muramic acid in cell wall.
“These rascals may microscopically lack colour.”
Giemsa stain
Borrelia
Plasmodium
Trypanosomes
Chlamydia
PAS (periodic acid-Schiff)
Stains glycogen, mucopolysaccharides; used to diagnose Whipple’s disease
Ziehl-Neelsen (carbol fusihsin)
Acid fast organisms
India ink
Crytococcus neoformans (Musicarmine
Silver stain
Fungi
Legionella
H influenzae culture requirements
Chocolate agar Factor V (NAD+) Factor X (hematin)
Neisseria Gonorrhea culture requirements
Thayer - Martin OR
VPN - Vancomycin, Polymyxin, Nystatin
Bordetella Pertuss
Bordet - Gengou (potato) agar
C. Diphtheriae
Tellurite Plate, Loeffler’s media
M. Tuberculosis
Lowenstein - Jensen agar (3-4 weeks to grow)
M. Pneumoniae
Eaton’s agar
Lactose fermenting enterics
MacConkey’s agar (pink colonies)
E. Coli also grown on EMB as blue black colonies with metallic sheen
Legionella
Charcoal yeast extract agar, buffered with cysteine and iron
Fungi
Sabourard’s agar
What organisms are obligate aerobes?
Nocardia
Pseudomonas aeruginosa
Mycobacterium Tuberculosis
Bacillus
Nagging pests must breathe!
What organisms are obligate Anaerobes?
Actinomycese
Bacteroides
Clostridium
What organisms are obligate intracellular?
Rickettsia
Chlamydia
What organisms are facultative intracellular?
Salmonella Neisseria Brucella Myobacterium Listerior Francisella Legionalla Yersinia Pestis
Some Nasty Bugs May live FacultativeLY
What are catalase positive organisms?
Staff Aureus Seratia Pseudomonas Actinomyces Candida E.Coli
SSPACE.
What are urease positive bugs?
Proteus Ureaplasma Nocardia Cryptococcus H. Pylori Klebshiella
PUNCH - K
5 bacterial toxins encoded on a lysogenic phage.
ShigA like toxin Botulinum toxin Cholera toxin Diphtheria toxin Erythrogenic toxin of Streptococcus Pyogenes.
ABCDE.
