Bacteriology

Question 1

Antibiotic agents classified as cell wall inhibitors
1. clarithromycin
2. Vancomycin
3. Tetracycline
4. Imipenem

A. 1 and 3
B. 2 and 4
C. 1,2,3
D. 1,2,3,4
E. None of these

Answer 1

E. None of these

vancomycin only

Question 2

Non-encapsulated N. meningitis
A. Throat swab
B. Nasopharyngeal swab
C. CSF
D. A and B

Answer 2

D. A and B

Question 3

Biosafety Lab mode of transmission gram + afb
A. BSL 1 B. BSL 2 C. BSL 3 D. BSL 4

Answer 3

C. BSL 3

Question 4

Choose the correct order of specimen - understaffed

Answer 4

Pericardial fluid
unpreserved stool
colony counting

Question 5

Delay CSF temp storage

Answer 5

37C

Question 6

Kirby Bauer
Intermediate- 12-14 mm
_________- 16 mm zone of inhibition result

Answer 6

susceptible

Question 7

Blood submitted for culture (+) S. aureus
A. Repeated since S. aureus is not significant
B. serial specimen must be collected
C. Communicated immediate
D. all of these

Answer 7

C. Communicated immediate

Question 8

Resazurin inside that contains inoculated plates must appear:

Answer 8

pink

Question 9

Repeated test for UTI that anaerobes agent
A. First morning
B. random urine
C. Catheterized
D. none of these

Answer 9

C. Catheterized

Question 10

Days of culture bottle for growth

Answer 10

7 days

Question 11

Sputum for mycobacteria detection must be treated with which of
the following prior to doing centrifugation?
A. NaOH
B. NACL
C. Z-TSP
D. Any of these

Answer 11

D. Any of these

Question 12

Which of the following cells that when noted microscopically would
confirm that specimen is a true respiratory sample
A. Columnar cells
B. Mast cells
C. Neutrophils
D. None of these

Answer 12

C. Neutrophils

Question 13

Which of the following that when noted in the laboratory will
warrant immediate communication of result to the clinician?
1. Surgical wound + streptococcus pyogenes
2. Positive acid fast stain
3. Gram stain suggestive of gas gangrene
4. Positive blood culture

A. 1 and 3
B. 2 and 4
C. 1, 2, 3
D. 1,2,3,4
E. None of these

Answer 13

B. 2 and 4

Question 14

When doing AST Kirby Bauer Disk method what is the maximum
number of antibiotic disks which may be applied to the surface of a single 150 mm Mueller Hinton Agar plate?

Answer 14

12 disks

Question 15

Which of the following antimicrobials are classified as Macrolides?
1. Clarithromycin
2. Gentamycin
3. Erythromycin
4. Imipenem

A. 1 and 3
B. 2 and 4
C. 1,2,3
D. 1,2,3,4
E. None of these

Answer 15

A. 1 and 3

Question 16

Kirby bauer Technique, too light inoculum and very dry agar can lead
to?
A. Smaller zone - false resistance
B. Smaller zone - false susceptible
C. Larger zone - false susceptible
D. Larger zone - false resistance

Answer 16

C. Larger zone - false susceptible

Question 17

are true of the D test for staphylococcus macrolide resistance except:
A. – and 2ug CC disk dispersed 15 mm apart
B. Detects inducible clindamycin resistance
C. — e zone constitutes to a positive result
D. Performed when macroslide results are discrepant

Answer 17

D. Performed when macroslide results are discrepant

Question 18

–to cause a false resistant result in disk diffusion method susceptible
testing except:
A. -
B. Too heavy inoculum
C. E-test
D. None of these

Answer 18

C. E-test

Question 19

Candle jar contain

Answer 19

5-10% CO2, 5% O2

Question 20

Inhibition of MIC and MBC

Answer 20

E-TEST

Question 21

Failure in gaspak jar:
A. presence air in colorless
B. Inactivation of catalyst due to repeated use
C. Both

Answer 21

B. Inactivation of catalyst due to repeated use

Question 22

– of the following materials may be used in specimen collection for
N. meningitidis except:
A. Calcium alginate
B. Swab made of Dacron
C. Swab made of rayon fibers
D. All of these

Answer 22

C. Swab made of rayon fibers

Question 23

The gene that activates resistance to clindamycin is
A. Emm gene
B. Erm gene
C. mecA gene

Answer 23

B. Erm gene

Question 24

Blood culture contaminants
A. S aureus
B. P acnes
C. C viridans
D. All of these

Answer 24

D. All of these

Question 25

Blood anticoagulant not suited for bacterial culture A. Citrate B. Heparin C. EDTA D. A and C

Answer 25

D. A and C

Question 26

105 colonies were counted on a plate media with a request of urine C/S, if a 1 uL loop was used during inoculation on media, what is the colony count/ml of urine and correct interpretation? A. 105,000 - may be considered UTI B. 10,500 - not UTI C. 115,000 - normal finding D. None of these

Answer 26

A. 105,000 - may be considered UTI

Question 27

All of the following are UTI agents except: A. S. pneumoniae B. K. pnuemoniae C. E. coli D. S. saprophyticus E. A and B

Answer 27

E. A and B

Question 28

Possible isolates in CSF culture 1. S. pneumoniae 2. S. agalactiae 3. N. meningitidis 4. H. influenza

Answer 28

ALL

Question 29

Semiquantitation technique involves streaking on

Answer 29

4 QUADRANTS

Question 30

Clindamycin result after a (+) D test A. Susceptible B. Resistant C. Intermediate D. Repeat D-test

Answer 30

B. Resistant

Question 31

Swarming on a Kirby Bauer plate must be

Answer 31

Ignore

Question 32

Which of the following agents must be added to a fungal media? 1. Cycloserine 2. Chloramphenicol 3. Cefoxitin 4. Cycloheximide

Answer 32

2 and 4

Question 33

What is gram staining reaction of fungi

Answer 33

Gram positive

Question 34

Which of the following can be used for aspergillus?

Answer 34

Czapeck Agar

Question 35

which can be used to stimulate chlamydospore formation of C. albcans?

Answer 35

Cornmeal Agar

Question 36

Which of the following permanent mounts can be used to demonstrate fungi in tissue samples? 1. H&E 2. Periodic Acid Schiff 3. Gomori’s methenamine silver 4. Fontana Masson A. 1 and 3 B. 2 and 4 C. 1,2,3 D. 1,2,3,4 E. None of these

Answer 36

E. None of these

Question 37

Demonstration of C. neoformans capsule may be accomplished thru the use of A. Calcofluor white B. Lactophenol cotton blue C. Acridine orange D. None of these

Answer 37

D. None of these