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Flashcards in Bacteriology outbreak cases Deck (21):
1

What is VIO?

Veterinary Investigation Officer

2

OCT?

Outbreak Control Team

3

CCDC?

Consultant in Communicable Disease Control

4

What is strange about 0157?

It is not reportable in animals, but it is in humans.

5

What are the 4 stages to a systematic approach for an outbreak?

1. Plan
2. Do.
3.Check
4. Act

6

What is the rule concerning prevalence and samples?

The lower the prevalence, the more samples need to be taken.

7

Name the 4 types of laboratory tests following sampling?

1. direct plating
2. Enrichment
3. Tissue culture
4. Molecular

8

What are the 4 steps of Serotyping/slide agglutination?

1. Transfer 10ul of sterile PBS to the slide using sterile loop
2. with fresh loop, pick a colony off Brilliant Green agar and resuspend in drop of PBS until you have a smooth suspenion
3. add single drop of poly-o Salmonella antisera onto the suspension
4. mix by rotating slide
5. any agglutination after 1-2 mins?

9

What is a sporadic outbreak?

Occurring irregularly, haphazardly, from time to time, and generally infrequently.

10

What is an outbreak?

An epidemic limited to localised increase in the incidence of disease

11

What is an endemic?

The constant presence of a disease in a geographic area or population group (Ro<1)

12

What is an epidemic?

Regional disease in excess of normal expectency (Ro >1)

13

What are the 4 most common foodborne pathogens?

1. campylobacter
2. clostridia perfringens
3. norovirus
4. salmonella

14

What is an OCP?

Outbreak control plan

15

What is the EFSA and what do you submit to them?

European Food Safety Authority -submit data on the final phase of an outbreak.

16

Where are salmonella more likely to be on birds?

In the feather follicles rather than the skin, this is why we use a stomacher.

17

What is RV-medium?

This is salmonella enrichment broth and selects salmonella over other enterobacteria

18

Name 4 features that enable salmonella to be selected for by RV-medium?

1. high osmotic pressure resistance
2. multiples in low pH
3. More resistant to malachite green
4. Low nutritional requirements

19

What colour does Brilliant Green Agar turn in low pH?

Turns yellow/green

20

Name 4 ways E.coli readily transfer resistance?

1. conjugation
2. transduction
3. transformation

21

Name 6 steps to an outbreak control plan?

1. system of communication and liason between the stakeholders
2. clear arrangement for media liason
3. care of patients and reduce person to person spread
4. provisions and necessary equipment needed
5. clear statement on the purpose of the plan in the control of food related incidents.