Flashcards in Bacteriology outbreak cases Deck (21):
What is VIO?
Veterinary Investigation Officer
Outbreak Control Team
Consultant in Communicable Disease Control
What is strange about 0157?
It is not reportable in animals, but it is in humans.
What are the 4 stages to a systematic approach for an outbreak?
What is the rule concerning prevalence and samples?
The lower the prevalence, the more samples need to be taken.
Name the 4 types of laboratory tests following sampling?
1. direct plating
3. Tissue culture
What are the 4 steps of Serotyping/slide agglutination?
1. Transfer 10ul of sterile PBS to the slide using sterile loop
2. with fresh loop, pick a colony off Brilliant Green agar and resuspend in drop of PBS until you have a smooth suspenion
3. add single drop of poly-o Salmonella antisera onto the suspension
4. mix by rotating slide
5. any agglutination after 1-2 mins?
What is a sporadic outbreak?
Occurring irregularly, haphazardly, from time to time, and generally infrequently.
What is an outbreak?
An epidemic limited to localised increase in the incidence of disease
What is an endemic?
The constant presence of a disease in a geographic area or population group (Ro<1)
What is an epidemic?
Regional disease in excess of normal expectency (Ro >1)
What are the 4 most common foodborne pathogens?
2. clostridia perfringens
What is an OCP?
Outbreak control plan
What is the EFSA and what do you submit to them?
European Food Safety Authority -submit data on the final phase of an outbreak.
Where are salmonella more likely to be on birds?
In the feather follicles rather than the skin, this is why we use a stomacher.
What is RV-medium?
This is salmonella enrichment broth and selects salmonella over other enterobacteria
Name 4 features that enable salmonella to be selected for by RV-medium?
1. high osmotic pressure resistance
2. multiples in low pH
3. More resistant to malachite green
4. Low nutritional requirements
What colour does Brilliant Green Agar turn in low pH?
Name 4 ways E.coli readily transfer resistance?