bacteriology_lab_20231208052941

Question 1

All forms of microbial life including spores and prions are killed

Answer 1

Sterilization

Question 2

Most resistant microbial form

Answer 2

Prions

Question 3

Inactive form of bacteria

Answer 3

Spores

Question 4

All pathogenic microorganisms, not necessarily all microorganisms, are killed

Answer 4

Disinfection

Question 5

Most common physical method of sterilization

Answer 5

Incineration

Question 6

Temperature for incineration

Answer 6

870-980

Question 7

Produced by incineration which are its drawback

Answer 7

Toxic fumes/metals

Question 8

Also known as principle of steam under pressure

Answer 8

Moist heat

Question 9

Physical method for heat stable objects

Answer 9

Moist heat

Question 10

What equipment is used in moist heat

Answer 10

Autoclave

Question 11

Most common type of autoclave

Answer 11

Gravity displacement

Question 12

Temperature, time, and pressure for routine method of moist heat which is used for media, liquids, and instruments

Answer 12

121 degree C, 15 mins, 15 psi

Question 13

Temperature and time for infectious medical wastes under moist heat

Answer 13

132 degree C for 30-60 mins

Question 14

Fastest, simplest, and most effective method for physical sterilization

Answer 14

Moist heat

Question 15

Bacillus steatothermphilus is the biological indicator for

Answer 15

Moist heat

Question 16

Sealed ampules is used to ensure that this method for sterilization is working. What colors indicate if the spores are killed or not?

Answer 16

Moist heatKilled= purpleNot killed= yellow

Question 17

Pressure sensitive autoclave tape will show black stripes when? (Sterilized or not)

Answer 17

Sterilized

Question 18

This is the alternate heating, incubation, and heating under moist heat

Answer 18

Fractional

Question 19

100°C for 30 mins for 3 consecutive days

Answer 19

Tyndallization

Question 20

Thickening through evaporation

Answer 20

Inspissation

Question 21

Inspissation temperature, and time

Answer 21

76°-80°C for 2 hrs for 3 days

Question 22

This is used for glasswares, oils, and powders

Answer 22

Dry heat

Question 23

Uses 160°-180° for 1.5-3 hrs

Answer 23

Dry heat

Question 24

Biological indicator for dry heat

Answer 24

Bacillus subtilis var nigar

Question 25

Technique used for dry heat (what instrument)

Answer 25

Wire loop

Question 26

Physical sterilization for heat sensitive objects

Answer 26

Filtration

Question 27

This physical sterilization method is used for antibiotic solutions, toxic chemicals, radioisotopes, vaccines, and carbohydrates

Answer 27

Filtration