Basic Flashcards by Zean Earl Carpeso

Bipolar bodies

Safety pin appearance in Wayson stain

Yersinia pestis

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Tackhead bacillus

Drumstick/Lollipop appearance

Clostridium tetani

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Tumbling motility in Hanging drop test

Listeria

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Darting motility in Hanging drop test

Campylobacter

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Gliding motility in Hanging drop test

Capnocytophaga

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Causative agent of Tetanus/Lock jaw

Clostridium tetani

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Toxin produced by Clostridium tetani that causes Tetanus and spasmodic contracions

Tetanospasmin (neurotoxin)

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Causative agent of Botulism

Clostridium botulinum

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Toxin produced by Clostridium botulinum that causes Botulism and flaccid paralysis

Botulinum toxin (neurotoxin)

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Pink to purple colonies with green metallic sheen on EMB

Escherichia coli

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Pink colonies with dark center on EMB

Fish eye colonies

Enterobacter

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The following are H2S (+) organisms except:
Salmonella
Proteus
Arizona
Corynebacterium
Edwardsiella

Corynebacterium

SPACEd
Salmonella
Proteus
Arizona
Clostridium
Edwardsiella

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Bulls eye colonies on CIN (Cefsulodin-Irgasan-Novobiocin)

Yersinia enterocolitica

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Exhibit satellitism, dew drop, satellite colonies around S. aureus
Mousy or mouse net smell, musty basement odor

Haemophilus influenzae

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Preferred medium for isolating Bordetella pertussis

Regan Lowe (enrichment and selective)

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Medusa head colonies

Bacillus anthracis

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Beaten egg consistency
Bamboo pole arrangement on culture
String of pearls on MHA with penicillin

Bacillus anthracis

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Molar tooth appearance

Actinomyces

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Fried egg appearance on culture
Lack cell wall
Smallest free living organism

Mycoplasma

No cell wall: Mycoplasma, Ureaplasma

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G(+) bacilli, non sporeforming, nonpigmented tan to buff colonies that are dry, warty, granular and rough resembling cauliflowers

Mycobacterium tuberculosis

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G(-) bacilli, obligate aerobe, grape like or corn tortilla like odor (production of 2-aminoacetaphenone) with serrated, confluent growth

Pseudomonas aeruginosa (swarming motility)

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All enterobacteriaceae are aerogenic except:

Shigella

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Biological indicator/Quality control in autoclave

Spores of Bacillus stearothermophilus

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Biological indicator/Quality control in oven, dry heat sterilization

Spores of Bacillus subtilis var. niger

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Biological indicator/Quality control in Ethylene Oxide (ETO)

Bacillus subtilis var. globigii

Biological indicator/Quality control in Ionizing radiation

Bacillus pumilis

Most abundant normal flora in a throat and nasopharyngeal specimen

Alpha hemolytic Strep: Viridans strep

Most common agent of UTI

Escherichia coli

Exhibits classic starbust pattern on gram stain | Resembles lymphocytes

Cryptococcus neoformans

G(+) cocci in grape like clusters | Creamy white pin head colonies on BAP

Staphylococcus

Beta zone of hemolysin on BAP

Staphylococcus aureus

Golden yellow growth on Loeffler's Serum Slant (LSS)

Staphylococcus aureus

Lemon yellow growth on Loeffler's Serum Slant (LSS)

Staphylococcus citreus

Porcelain white growth on Loeffler's Serum Slant (LSS)

Staphylococcus albus/ | Staphylococcus epidermidis

Flame/Bullet/Lancet shaped diplococci on Gram stain

Streptococcus pneumoniae

Causative agent of Lobar pneumonia with rusty sputum

Streptococcus pneumoniae

Alpha hemolytic on BAP | Causative agent of Subacute Bacterial Endocarditis (SBE)

Viridans streptococci

Viridans strep associated with dental carries

Streptococcus mutans

Nutritionally Variant Streptococci (NVS) | Need cysteine/pyridoxal (vit B6)

Abiotrophia spp.

G(-) diplococci resembling coffee beans or kidney beans

Neisseria sp. except N. elongata (rod shaped)

Causative agent of ophthalmia neonatorium in newborns

Neisseria gonorrhoeae

Causative agent of Waterhouse-Friderichsen syndrome: hemorrhage in adrenal gland

Neisseria meningitidis

Causative agent of Montezumas revenge, turista, travelers and childhood diarrhea Profuse watery stool

Enterotoxigenic Escherichia coli (ETEC)

``` Causes a Shigella like infection A. ETEC B. EIEC C. EHEC D. Shigella ```

Enteroinvasive Escherichia coli (EIEC)

Friedlander's bacillus

Klebsiella pneumoniae

Exhibits a rancid potato-like odor | DNAse, Gelatinase and Lipase (+)

Serratia odorifera

Causative agent of Enteric/Typhoid fever: Pea soup stool

Salmonella

Causative agent of Bacillary dysentery

Shigella dysenteriae (Shigellosis)

Non spore forming g(-) anaerobic rods, lysine deamination and MR (+) Exhibit swarming motility

``` Proteus mirabilis (OX-K): putrid odor Proteus vulgaris (OX-2, OX-19) ```

Causative agent of Black death Stalactite pattern on broth culture Exhibits a Safety pin appearance due to bipolar bodies inclusion on Wayson stain

Yersinia pestis

Old sock odor on Mannitol Salt agar

Staphylococcus aureus

Freshly plowed field odor

Nocardia sp.

Causative agent of Swimmer's ear: Mild otitis externa/media

Pseudomonas aeruginosa

Causative agent of Ecthyma gangrenosum: skin lesions | Hot tub/Whirlpool dermatitis

Pseudomonas aeruginosa

Causes respiratory infections in patients with cystic fibrosis

Burkholderia cepacia

Causative agent of Glanders disease: a disease of horses transmittable to humans

Burkholderia mallei

Causes mellioidosis: Glanders like Whitmore's bacillus Vietnamese Time Bomb (can reactivate after 9years)

Burkholderia pseudomallei

Motile G(-) anaerobic bacilli, oxidase (+) Produces Choleragen toxin causing Rice water stool "Fish in the stream" appearance Yellow colonies on TCBS (sucrose fermenter)

Vibrio cholerae

G(-) anaerobic comma shaped bacilli

Vibrio sp.

Pfeiffers bacillus | Causes meningitis in children <5 yrs old

Haemophilus influenzae

Formerly known as Koch weeks bacillus | Causative agent of Pink Eye Conjunctivitis

Haemophilus aegypticus

Smallest pathogenic bacilli Infective agent of Soft chancre, chancroid School of fish arrangement

Haemophilus ducreyi

Bacteria that can grow at 42-43C

Pseudomonas aeruginosa | Campylobacter

S-shaped G(-) bacilli resembling "Sea gulls wings" appearance associated with gastritis and diarrhea exhibiting darting motility by single polar flagella

Campylobacter

G(-) bacilli, optimum temp for growth at 35-37C, motile with 3-6 flagella Associated with peptic ulcer

Helicobacter

Causative agent of Whooping cough | Mercury droplet colonies

Bordetella pertussis

Non motile, G(-) aerobic cocobacilli | Causative agent of Brucellosis, undulant fever, Malta fever

Brucella

Causative agent of Legionnaire's disease, Pontiac fever

Legionella pneumophila

Causative agent of Pittsburgh pneumonia

Legionella micdadei

Wiga's agent of pneumonia

Legionella bozemanni

Causes Shipping fever in cattles | Causative agent of Pasteurellosis

Pasteurella multocida

Obligateky aerobic non motile, faintly staining g(-) coccobacilli Causes Tularemia

Francisella

Associated with bacterial vaginosis, clue cells in cytology/Paps Fishy amine like odor in Whiff/Sniff test

Gardnerella vaginalis

G(-) non spore forming bacteria Causative agent of: -Rat bite fever: Spirillum minor: from animal bite -Haverhill fever: from ingestion of contaminated milk

Streptobacillus moniliformis

G(-) capnophilic, fusiform or filamentous bacilli exhibiting gliding motility

Capnocytophaga

Pleomorphic, Encapsulated g(-) bacilli | Causative agent of Granuloma Inguinale or Donovanosis

Calymmatobacterium granulomatis

Corroding bacterium with characteristic smell of bleach on agar

Eikenella corrodens

Spore forming bacteria

Bacillus | Clostridium

``` Aerobic G(+) bacill, catalase (+) Causative agent of Cutaneous anthrax Pulmonary anthrax (Woolsorter's disease) Gastrointestinal anthrax ```

Bacillus anthracis

``` Causative agent of myonecrosis (gas gangrene) Exhibits double zone of hemolysis Reverse CAMP (+) Lecithinase (+) Nagler reaction (+) ```

Clostridium perfringens

Agent of food (canned goods), wound, and infant botulism "Floppy baby"

Clostridium botulinum

Causes spastic contractions and lock jaw

Clostridium tetani

Cause of antibiotic-associated pseudomembranuos colitis (Clindamycin: 1st antibiotic) Cultured on CCFA (Cylcoserine Cefoxitin Fructose Agar)

Clostridium difficile

Klebs-Loeffler bacillus

Corynebacterium diphtheriae

Club shaped, dumb bell shaped, Parallel, Palisading, Picket fence, Chinese character appearance

Corynebacterium sp,

Multidrug resistant diphthteroid associated with endocarditis, pneumonia, perotonitis

Corynebacterium jeikeium | Formerly group JK

Basic Flashcards

(86 cards)