Basic Chromatography Flashcards
(48 cards)
is the ability to separate molecules using various partitioning characteristics of molecule to remain in a stationary phase versus a mobile phase. Once a molecule is separated from the mixture, it can be isolated and quantified.
Chromatography
Can chromatography identify components?
No, it needs a detector.
It always need a detector to identify
chromatographically the separated components. In liquid chromatography,
detector response is NOT a determining
factor for the column and thin-layer efficiencies.
It was first applied by ____________ for the
separation of plant pigments.
Mikhail Semyonovich Tsvet
It was first applied by Mikhail
Semyonovich Tsvet for the ________.
separation of plant pigments
Greek chromatos means
Color
Greek graphein means
To write
Two Major Components of Chromatographic Separation
• Mobile Phase
• Stationary Phase
flows through the column; carries analyte
Mobile Phase
stays in place, does not move
Stationary Phase
instrument employed for a chromatography.
Chromatograph
phase that stays in place inside the column. It can be a particular solid or gel-based packaging (LC) or a highly viscous liquid coated inside the column (GC)
Stationary Phase
the solvent moving through the column; either a liquid in LC or gas in GC.
Mobile Phase
fluid entering the column
Eluent
fluid exiting the column
Eluate
is the process of passing the mobile
phase through the column.
Elution
graph showing detector response as a function of time.
Chromatogram
defines how much mobile phase
passed per minute (mL/min)
Flow rate
distance passed by mobile phase per 1 min in the column (cm/min)
Linear velocity
Rf value of Beta carotene
0.99
Rf value of Chlorophyll A
0.30
Rf value of Chlorophyll B
0.13
Rf value of Violaxanthin
0.40
Rf value of Lutein
0.68
The higher the Rf, the ____ polar
is the solute.
The lower the Rf, the _____ polar is
the solute.
less; more (Rf value is inversely proportional to polarity)
