BASIC HEMATOLOGICAL METHODS OF EXAMINATION

Question 1

Basic screening test and most frequently ordered laboratory procedure

Answer 1

CBC or Complete Blood Count

Question 2

Measurement of the concentration of Hgb in the blood

Answer 2

Hemoglobin Determination

Question 3

Used to screen for disease associated with anemia and to evaluate polycythemia

Answer 3

Hemoglobin Determination

Question 4

test indirectly measures
the RBC mass

Answer 4

Hematocrit Determination

Question 5

Results are expressed as the percentage by volume of packed RBCs

Answer 5

Hematocrit Determination

Question 6

series of test that determines number , variety, percentage concentration and quality of blood cells

Answer 6

CBC

Question 7

valuable diagnostic information about the hematologic and other body system , prognosis response to treatment and recovery

Answer 7

CBC

Question 8

Hgb, Hct, RBC and WBC count , Differential Count

Answer 8

CBC

Question 9

Important buffer in the extracellular fluid

Answer 9

Hemoglobin

Question 10

–main component of erythrocytes

Answer 10

Hemoglobin

Question 11

–serves as the vehicle for the transportation of oxygen and carbon dioxide

Answer 11

Hemoglobin

Question 12

Ratio of the volume of packed RBCs to the volume of whole blood

Answer 12

Hematocrit or Packed Cell Volume

Question 13

blood test used to find out how many RBCs

Answer 13

RBC COUNT or Erythrocyte Count

Question 14

Increase RBC

Answer 14

Polycythemia Vera

Question 15

Decrease RBC

Answer 15

Anemia

Question 16

measures the number of WBC and may serve as guide to the severity of the disease process that detects hidden infection

Answer 16

WBC Count or Leukocyte Count

Question 17

WBCs may be counted visually using a microscope and ____________

Answer 17

HEMACYTOMETER

Question 18

WBC COUNT

dilution is _______ and the diluent is a dilute _______

Answer 18

1:20—acid solution

Question 19

detect immature WBC and abnormalities

Answer 19

DIFFERENTIAL COUNT

Question 20

total count of circulating WBC is differentiated according to the five types of leukocytes

Answer 20

NEBLM

Question 21

pyogenic infection

Answer 21

neutrophils

Question 22

Parasitic infection

Answer 22

Eosinophils and Basophils

Question 23

allergic disorder

Answer 23

Eosinophils

Question 24

Viral infection

Answer 24

Lymphocytes

Question 25

severe infection by phagocytosis

Answer 25

Monocytes

Question 26

Use to assess the erythropoietic activity of the bone marrow

Answer 26

Reticulocyte count

Question 27

Reticulocyte Count –whole blood, anticoagulated with EDTA is stained with a supravital stain such as __________ or __________

Answer 27

new methylene blue—brilliant cresyl blue

Question 28

Rate at which red blood cells sediment in a period of one hour

Answer 28

Erythrocyte Sedimentation Rate (ESR)

Question 29

Common hematology test and nonspecific measure of inflammation

Answer 29

Erythrocyte Sedimentation Rate (ESR)

Question 30

___________ is one of the oldest hematology tests and detects inflammation and roughly estimates its intensity

Answer 30

ERYTHROCYTE SEDIMENTATION RATE (ESR)

Question 31

What test uses graduated concentrations of saline solutions to detect SPHEROCYTES (RBCs with proportionally reduced surface membrane area) in hereditary SPHEROCYTOSIS or WARM AUTOIMMUNE HEMOLYTIC ANEMIA?

Answer 31

OSMOTIC FRAGILITY TEST

Question 32

Osmotic fragility test uses graduated concentrations of saline solutions to detect ___________ (RBCs with proportionally reduced surface membrane area) in hereditary ___________

Answer 32

SPHEROCYTES—SPHEROCYTOSIS or WARM AUTOIMMUNE HEMOLYTIC ANEMIA.

Question 33

phenotypically detects an inherited RBC enzyme deficiency causing severe episodic hemolytic anemia

Answer 33

GLUCOSE-6-PHOSPHATE DEHYDROGENASE ASSAY

Question 34

Sickle cell solubility screening assay confirmatory TESTs

Answer 34

1. HEMOGLOBIN ELECTROPHORESIS 2. HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Question 35

Detect and diagnose sickle cell anemia and other inherited qualitative hemoglobin abnormalities and thalassemia's

Answer 35

SICKLE CELL SOLUBILITY SCREENING ASSAY or HEMOGLOBIN ELECTROPHORESIS and HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Question 36

Platelet Parameters included in the complete blood count (CBC)

Answer 36

Platelet Count and Mean Platelet Volume MPV

Question 37

High WBC

Answer 37

leukocytosis

Question 38

Low WBC

Answer 38

Leukopenia

Question 39

increased RBC count

Answer 39

polycythemia vera

Question 40

decreased RBC and cause of malfunction in the bone marrow

Answer 40

ANEMIA

Question 41

2 Methods of Hgb for manual counting

Answer 41

1. Cyanmethemoglobin method (HiCn) 2. Sahli's acid-hematin method

Question 42

True or False Differential count can be done with chemotherapy patients

Answer 42

False—Can't be done with chemotherapy patients since the treatment destroys their cells → 100 cells can’t be identified

Question 43

Healthy individuals WBC Count

Answer 43

100 or 5.75 x 10^9/L

Question 44

Whole blood, anticoagulated with EDTA is stained with a _________ that stains the living cells.

Answer 44

supravital stain

Question 45

Supravital stains used:

Answer 45

1. New methylene blue 2. Brilliant cresyl blue 3. Pure azure blue

Question 46

disposable and most common method to measure the ESR

Answer 46

Westergren method

Question 47

Method used to measure the ESR and HCT

Answer 47

Wintrobe method

Question 48

Also known as sedrate

Answer 48

Erythrocyte Sedimentation Rate (ESR)

Question 49

Erythrocyte Sedimentation Rate (ESR) unit of measurement

Answer 49

mm/hr