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Flashcards in Basic Knowledge Deck (20):
0

Plus end dependent microtubule motor

Kinesins

1

Minus end dependent microtubule motor

Dynein

2

~How many superfamily members does kinesin have?

45

3

What filament does myosin move along?

Actin filaments

4

Motor that transports from cell periphery to cell center?

Dynein

5

Does dynein or dynactin contain p150glued?

Dynactin

6

Is plus end toward the axon terminal or toward the cell body?

Toward the axon terminal

7

SMN1

Spinal muscular atrophy protein

8

What happens when dynamintin is over-expressed in postnatal mice?

It disrupts dynactin, leading to slowly progressive motor neuron degeneration (Lamonte et al, 2002)

10

Perry syndrome

A rare disorder characterized by Parkinsonianism, psychiatric changes, hypoventilation, and suicidal thoughts

11

Hereditary spastic paraplegia

A group of upper motor neuron neurodegenerative disorders characterized by progressively worsening lower limb weakness and spasticity, sometimes accompanied by sensory deficits and bladder dysfunction.

12

What are some things that dynein regulates?

1. Axonal transport. 2. Endosomal trafficking. 3. Spindle formation. 4. Mitosis.

13

Is p150glued the largest subunit of dynactin?

Yes

14

What are the three relevant p150glued mutations that we have identified?

S263I, H668Y, P811R

15

Is CAP-Gly part of dynactin or dynein?

It is part of dynactin! Remember that CAP-Gly and dynactin both have an A

16

Mutations in lis1 leads to?

Lissencephaly

17

DMEM

Dulbecco's modified eagle medium - mammalian cell culture medium. Contains amino acids, salts, glucose, and vitamins. Our lab uses the DMEM with glucose and L-glutamine, without sodium pyruvate.

18

FBS

Fetal bovine serum; the blood fraction remaining after natural coagulation of blood, following centrifugation to remove RBCs.

19

Glutamax

Cell culture media that contains dipeptide from L-glutamine (L-ananyl-L-glutamine - this prevents degradation and ammonia buildup during long-term cultures). "GlutaMAX formulations (there are two) are dipeptides, which are cleaved in
cells by aminopeptidases, releasing L-glutamine from the dipeptide, either
L-alanine (GlutaMAX I), or glycine (GlutaMAX II)" Results in growth that is better than media supplemented with glutamine.

20

DPBS

Dulbecco's phosphate buffered saline. pH 7.2-7.6.