Basic Overview of generation of siRNAs and miRNAs and their mode of action Flashcards Preview

Biochemistry 431 > Basic Overview of generation of siRNAs and miRNAs and their mode of action > Flashcards

Flashcards in Basic Overview of generation of siRNAs and miRNAs and their mode of action Deck (10):
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# 1

(siRNA) derived from dsRNA comes from virus or lab ; (miRNA) derived from nuclear pre-miRNA

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#2

Dicer complex - cleaves dsRNA into smaller version

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# 3

Dicer transfers RNAs to risc

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# 4

Risc- separates small dsRNA into two strands. 1 strand is degraded 1 is left over

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#5

Risc guides small RNAs to mRNAs in the cell that are homologous (high sequence identity)

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Argonaute proteins

use small RNAs as guides to find complementary mRNA

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# 6 Function of miRNA

Not exact complementary (translationally inhibits mRNA)

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# 6 Function of siRNA

has good complementary; binds mRNAl perfect pairing (Uses Risc's nuclease activity cutting mRNA)

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Function of Dicer

Endoribonnuclease; forms miRNA and siRNA; facilitates the formation of RISC

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Risc

RNA-induced silencing complex ; bind ss siRNA and miRNA

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