Basics of Chromatography Flashcards
the ability to separate molecules using various partitioning characteristics of molecule to remain in a stationary phase versus a mobile phase.
Chromatoraphy
Once a molecules is separate from teh mixture it can be _______ and _______.
isolated and quantified
Can chromatography identify components?
No, it needs a detector
In liquid chromatography detector response is ____ a determeing factor for the colum and thin layer efficiencies
Not
Who applied chromatography first in the separation of plant pigments?
Mikhail Semyonovich Tsvet
Greek for color
chromatos
Greek for to write
graphein
What are the major components of chromatography separation
Mobile Phase
Stationary Phase
flows through the column; carries analyte
Mobile Phase
stays in place, does not move
Stationary Phase
instrument employed for chromatography
Chromatograph
Phase that stains in place inside the collumn.
Stationary phase
It can be a
particular solid or gel-based packaging
(LC) or a highly viscous liquid coated
inside the column (GC)
Stationary Phase
the solvent moving
through the column; either a liquid in LC
or gas inGC.
Mobile Phase
fluid entering the column.
Eluent
fluid exiting the column.
Eluate
is the process of passing the mobile
phase through the column.
Elution
graph showing detector
response as a function of time.
Chromatogram
defines how much mobile phase
passed per minute (mL/min)
Flow Rate
distance passed by mobile
phase per 1 min in the column (cm/min)
Linear Velocity
What are the types of Chromatography?
Based on the Bed
Based on the Physical State Phases
Based on the Separation Mechanism
Enumerate the based on the Bed chromatography
Column Chromatography
Planar Chromatography
Enumerate the chromatography based on the Physical State phases
Gas chromatography
Liquid Chromatography
Enumerate the chromatography based on the Separation mechanism
Affinity chromatography
Ion-exchange chromatography
Gel-Filtration chromatography
