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Flashcards in BB Ch21 Deck (29)
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1

2) The most difficult parameter to control is:
a. Temperature
b. Cage size
c. Humidity

c

2

3) An environmental factor that may influence experimental data is:
a. Physical
b. Chemical
c. Microbial
d. All of the above

d, duh

3

4) T or F A dual-corridor circulation pattern in the animal caging area is better than a single-corridor pattern to reduce chances of cross contamination.

True

4

5) The most common cubical size is:
a. 4 feet deep by 6 feet wide
b. 7 feet deep by 7 feet wide
c. 7 feet deep by 10 feet wide

a

5

6) What percentage of the mobile cage racks should the dirty side of the cage sanitizer hold?

at least 10%

6

7) The maximum temperature that feed from natural sources should be stored at is:

70 F (21 C)

7

8) T or F An ABSL-2 room holding nonhuman primates should maintain negative pressure relative to the corridor.

True, duh

8

9) What does the acronym HEPA stand for?

High Efficiency Particulate Air

9

10) The official definition of a HEPA filter is a filter that removes:

99.97% of particles measuring 0.3 um in diameter

10

11) T or F Air supplied to animal rooms should be 100% outside air.

True

11

12) In a single-corridor conventional facility holding clean and dirty animals, the rooms with the “clean” animals should have a (positive or negative) air balance?

positive

12

13) The standard design temperature range for animal rooms is:

65-85 F

13

14) The generally accepted range for relative humidity control is:

30-70%, duh

14

15) T or F The “Guide” recommends that rooms housing albino animals be approximately 325 lux (30 foot-candles) at 1.0 m (3.3 ft) above the floor. This level of light appears to be adequate for routine animal care.

True (verify)

15

16) Floor drains in animal rooms should be a minimum of what size?

4” for rodent rooms and 6” for rooms that flush waste (verify)

16

What is the minimum recommended ceiling height?

9 ft
May need to be taller if rodent caging is expected to exceed 7-8 ft.

17

18) The recommended door size for rooms housing nonhuman primates is:

42" wide x 8' tall

18

T or F Fire alarms that operate between 400 kHz and 500 kHz should be used in facilities housing rodents because most rodent species cannot hear frequencies below 1000 kHz.

True

19

According to the “Guide”, corridors should be:

6-8 ft wide

20

21) Sanitation can be achieved at:

180° F for 1 second or
161° F for 15 seconds or
143° F for 30 minutes

21

22) T or F Hyperchlorination has been widely used in the past, but the most common water treatment used for controlling microbial contaminants delivered via water bottles is acidification to a pH of 2.5 -3.0.

True

22

23) T or F The standard practice is to change cages 1 time per week when using a ventilated microisolation caging system.

True

23

Which type of Biological Safety Cabinet is most commonly used in research animal facilities?

Class II

24

25) T or F It is required by the Animal Welfare Act that training must be provided for personnel caring for laboratory animals.

True, duh

25

26) The LAM book recommends that SOP’s be reviewed how often?

b. Preferably every 6 months, but no less that once a year

26

27) Name 2 types of cage washers used.

27) Tunnel, Batch washers

27

28) Name 3 types of animal diets.

28) Closed formula, Open formula, Certified

28

29) What are the two main types of bedding used?

29) Contact, Non-contact

29

30) In general, how often should animal waste be washed down and primary enclosures sanitized?

Wash down runs daily, Sanitize every 2 weeks