BE HTMLE Flashcards

(66 cards)

1
Q

Best fixative for the nervous system

A

FORMALIN FIXATIVE

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2
Q

Fixative of choice for the preservation of fats

A

Formalin fixative

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3
Q

Helly s fluid is exactly the same as in Zenker’ s fixative, but instead of GLACIAL ACETIC ACID, what component is added

A

Formalin

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4
Q

Fixative recommended for fixing small pieces of liver, spleen, connective tissue fibers and nuclei

A

Zenker Fluid

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5
Q

Excellent microanatomic fixative for pituitary gland, bone marrow and blood containing organs such as spleen and liver

A

Hellys

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6
Q

All mercurial fixing solutions lead to the formation in tissues of diffuse BLACK granules and these mercury deposits must be removed BEFORE STAINING. Removal of mercuric chloride deposit is accomplished by

A

Saturated solution of iodine

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7
Q

Considered to be the MOST RAPID fixative/ recommended for fixing chromosomes, lymph glands and urgent biopsies:

A

Carnoys

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8
Q

Most widely used fixative for electron microscopy

A

Lead fixative

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9
Q

The fastest chemical solution in decalcifying tissues is:

A

Nitric acid

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10
Q

All of the following remove intracellular and extracellular water from the tissue following fixation, EXCEPT:
A. Alcohol
C. Tetrahydrofuran
B. Chloroform
D. Dioxane

A

B

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11
Q

A TOXIC dehydrating agent, primarily employed for blood and tissue films and for smear preparation:
A. Ethyl alcohol
C. Butyl alcohol
B. Methyl alcohol
D. Isopropyl alcohol

A

B

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12
Q

Function/s of tetrahydrofuran:

A

Dehyrating agent Clearing agent

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13
Q

All of the following are substitutes for paraffin wax, EXCEPT:
A. Paraplast
B. Embeddol
C. Malinol
D. Tissue Mat

A

C

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14
Q

Melting point of ester wax:

A

46-48C

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15
Q

Microtome knife recommended for frozen sections or for cutting extremely hard and tough specimens embedded in paraffin blocks, using a base-sledge type or sliding microtome:

A

Plane wedge knife

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16
Q

Angle formed between the cutting edge of the microtome knife
(27o-32o)

A

Bevel angle

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17
Q

Angle formed between the surface of the block and the cutting edge of the knife (0o-15o)

A

Clearance angle

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18
Q

This type of microtome easily cuts large blocks and serial sections can be obtained with ease because larger knives can be used

A

Sliding

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19
Q

A tissue exposed to short burst of CARBON DIOXIDE for a few minutes will

A

Freeze

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20
Q

Fat cells and enzymes are best demonstrated in:

A

Frozen section

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21
Q

When trimming tissue block, they must be surrounded by at least __ of wax

A

2mm

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22
Q

The following methods are done for drying sections on slide, EXCEPT:
A. On a hot plate at 45-55oC for 30-45 minutes
B. On a Bunsen flame
C. In an incubator at 37oC for 3 hours
D. In a wax oven at 56-60oC for 2 hour

A

C

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23
Q

Adhesive added to the water in the floating-out bath most convenient alternative to direct coating of slide

A

Gelatin

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24
Q

Process by which sections are stained with simple aqueous or alcoholic solutions of the dye:

A

Direct staining

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25
A tissue-mordant-dye complex is needed in
Indirect staining
26
With this staining technique, the tissue is first overstained, and the excess stain is removed or decolorized from unwanted part of the tissue
Regressive staining
27
All of the following are metachromatic stains, EXCEPT: A. Thionine C. Safranin B. Toluidine blue D. Eosin
D
28
Stain used for demonstrating mitochondria during intravital staining
Janus Green B
29
Application of a different color or stain to provide contrast and background to the staining of the structural component to be demonstrated
Counterstaining
30
The routine stain for surgical tissue section is:
H & E
31
Routinely used in histopathology as a counterstain/background fro contrasting stain because it gives a pleasing and colorful contrasts to nuclear stains
Eosin
32
In routine H&E, most fixatives can be used, EXCEPT: A. Zenker s formol C. Osmic acid B. Formaldehyde D. Picric acid
C
33
Only substance in histopathology that can fix, differentiate or stain tissues all by itself:
Picric acid
34
The active dye (coloring agent) in hematoxylin solution is
hematin
35
All of the following are chemical oxidizing agent/ripening agent for hematoxylin, EXCEPT: A. Sodium iodate C. Hydrogen peroxide B. Mercuric oxide D. Ammonium alum
D
36
Ripening agent for Harris hematoxylin
Mercuric oxide
37
Function of ammonium alum in the Harris hematoxylin formula:
Mordant
38
Staining of the nuclei by alum hematoxylin is enhanced by the addition of:
Glacial acetic acid
39
A sheen of oxidized dye on the surface of hematoxylin solution indicate
stain has to be filtered
40
The acid used in combination with alcohol in an acid alcohol solution
Hydrochloric acid
41
Staining result in the routine hematoxylin and eosin method
Nuclei blue to blue-black, cytoplasm pink
42
A common BASIC NUCLEAR STAIN for plasma cells and may also be employed in cytological examination of fresh sputum for malignant cells:
Methylene blue
43
PAS positive substances are stained
Magenta red
44
Staining method for glycogen: A. PAS technique C. Best Carmine method B. Langhan s iodine stain D. All of these
D
45
Tubercle bacilli in Ziehl-Neelsen stain:
Bright red
46
Feulgen s reaction is the most reliable and most specific histochemical staining technique for:
DNA
47
In the Masson-Fontana ammoniacal silver reaction, melanin and argentaffin cell granules are stained
Black
48
Positive reaction for cholesterol in Schultz Method:
Blue-green
49
Components of the EA-50 stain, EXCEPT: A. Eosin Y C. Light green B. Orange green D. Bismarck brown
B
50
All of the following are fixative used for cytologic smears, EXCEPT: A. Equal parts of 95% ETOH and ether C. 10% Formalin B. 95% Ethyl alcohol D. Carnoy s fluid
C
51
BEST fixative for virtually all diagnostic cytology studies but flammable and volatile
Equal parts of 95% ETOH and ether
52
All of the following are examples of good adhesive agents for cytologic method, EXCEPT: A. Egg albumin C. Pooled human serum or plasma B. Celloidin ether alcohol D. Leuconostoc culture
A
53
Smears prepared for cytologic examination from cut surfaces of lymph nodes can be obtained by
Touch preparation
54
Organisms found in the normal vaginal flora that stain BLUE to lavander with Pap s method:
Doderlain bacilli
55
Cells found in a vaginal smear which are thick and round to oval in shape (similar to fried fresh eggs with sunny-side up) with strongly basophilic cytoplasm and vesicular nucleus
Parabasal
56
Increase in size of an organ or tissue due to an increase in number of cells
Hyperplasia
57
The dissolving of cells by enzymatic action is
Autolysis
58
A malignant tumor is least characterized by: A. Metastases C. Encapsulation B. Mitotic figures D. Invasion of adjacent tissues
C
59
The Blood Banking Law
RA 1517
60
National Blood Services Act of 1994:
RA 7719
61
Philippine AIDS Prevention and Control Act of 1998
RA 8504
62
Newborn Screening Act of 2004
RA 9288
63
All services are done on a primary category laboratory except: (A.O. 2007-0027) A. Routine urinalysis C. Routine chemistry B. Routine hematology D. Blood typing
C
64
Who are the members of the Medical technology Board?
A registered pathologist and two registered medical technologist
65
Sections of RA 5527 amended by PD 1534
3 8 13
66
The penalty of revocation of certificate of registration may be imposed by the board if there is
Unanimous vote