Beckman Coulter Instrument

Question 1

Electrical Impedance

Answer 1

Detection and measurement of changes in electrical resistance
Cells pass through a small aperature via gentle vacuum
As cells pass through current, they create resistance due to electrical impedance

Question 2

Impedance instrument

Answer 2

2 electrodes (internal, external)
Low frequency
Isotonic diluent (cell suspension)

Question 3

Number of voltage pulses

Answer 3

Cell count

Question 4

Length/height of voltage pulse

Answer 4

Cell size

Question 5

Spectrophotometry

Answer 5

Measures hemoglobin
Methemoglobin binds cyanide
CYANMETHEMOGLOBIN
540 nm