Beer Lambert Law Flashcards
1
Q
The amount of incident light absorbed by a solution depends on?
A
- thickness, 2. concentration of the absorbing compound in the solution, 3. Chemical nature of the absorbing compound
2
Q
What is E?
A
Extinction coefficient (measure of the extent of a particular molecule to absorb light of a certain wavelength (m-1 cm-1)
3
Q
What is c?
A
Concentration of absorbing species (M)
4
Q
What is l?
A
Path length (cm)
5
Q
What is the beer lambert law?
A
A = E c l or log lo/l.
Lo= incident light, l = transmitted light
6
Q
Absorbency is a linear function of concentration up to?
A
Od = 3.0
7
Q
All absorbance measurements should be made relative to a _____
A
Blank
8
Q
The blank contains?
A
All components of the Assay except the compound being measured
