Bio 111L: Microscopes

Question 0

Compound microscope definition

Answer 0

Puts light THROUGH specimen fixed to glass slide. 2 lens. Higher mag. Lower res.

Question 1

Stereoscopic/dissecting definition

Answer 1

Reflects light from surface of specimen. One lens. Lower mag. Higher res. used to view parts or entire organisms. 300x

Question 2

Scanning definition

Answer 2

Electrons bounce off gold coated specimen for 3D image. Views external features of small objects. 100,000x

Question 3

Transmission definition

Answer 3

Electrons sent through thin slice of specimen to create 2D image 100,000x

Question 4

Cost of compound

Answer 4

$150-1500

Question 5

Cost if stereoscopic

Answer 5

$150-10,000

Question 6

Scanning cost

Answer 6

$50,000

Question 7

Transmission cost

Answer 7

$50,000

Question 8

What objective should the microscope be at when ready to put back on shelf?

Answer 8

4x

Question 9

What should u feel when changing objectives?

Answer 9

CLICK.

Question 10

What is resolution?

Answer 10

Smallest degree of separation at which 2 objects appear distinct

Question 11

How do you calculate total magnification?

Answer 11

Multiply power of OBJECTIVE & power of OCULAR lenses

Question 12

What is parfocal?

Answer 12

When image is focused on low lower, it should still be focused on high

Question 13

What is a compound microscope used for?

Answer 13

To view cells and bacteria at 2000x

Question 14

Transmission purpose

Answer 14

Views internal features of small objects