BIO Flashcards

(71 cards)

1
Q

Explain Nitrogen Fixation in the nitrogen cycle

A

Nitrogen gas (N2) makes up about 78% of the Earth’s atmosphere, but it is in a form that most organisms cannot use directly. Nitrogen-fixing bacteria convert atmospheric
nitrogen into ammonium (NH4+), which is a form of nitrogen that plants can take up and use.

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2
Q

Explain Nitrification in the nitrogen cycle

A

Ammonium (NH4 + ) can be converted into nitrite (NO 2 - ) and then into nitrate (NO 3 - ) by
nitrifying bacteria. This step is important because nitrate is the primary form of nitrogen taken up
by most plants.

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3
Q

Explain Denitrification in the nitrogen cycle

A

Denitrifying bacteria convert nitrate (NO 3 - ) and nitrite (NO 2 - ) back into nitrogen gas (N 2 ) or nitrous oxide (N 2 O).

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4
Q

Why is denitrification important?

A

This process is essential for returning nitrogen to the atmosphere, closing the nitrogen cycle and preventing the accumulation of excess nitrates in the environment, which can have negative environmental impacts.

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5
Q

Explain ammonification in the nitrogen cycle

A

Microbes, such as bacteria and fungi, break down organic matter, including dead plants and animals, and convert the nitrogen compounds within them into ammonium (NH4+). This makes nitrogen available for reuse by plants.

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6
Q

What is the carbon cycle?

A

The carbon cycle is the natural process by which carbon moves between the Earth’s atmosphere, oceans, soil, plants, and animals.

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7
Q

Explain decomposition in the Carbon cycle

A

Microbes are key players in the decomposition of organic matter. During this decomposition process, carbon in the form of organic compounds is converted into carbon dioxide (CO2) and returned to the atmosphere.

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8
Q

Explain photosynthesis in the Carbon cycle

A

Microscopic photosynthetic organisms, such as phytoplankton in the ocean, capture carbon dioxide from the atmosphere and convert it into organic matter, such as carbohydrates, through photosynthesis.

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9
Q

Explain respiration in the Carbon cycle

A

Microbes, along with plants and animals, participate in respiration, releasing carbon dioxide back into the atmosphere. During respiration, organic compounds are broken down to produce energy, and carbon is released as CO2.

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10
Q

Explain carbon sequestration in the carbon cycle

A

Certain microbes (in soil), can contribute to carbon sequestration. They can help stabilise and store organic carbon in the soil, reducing the amount of carbon released into the atmosphere.

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11
Q

Explain methane production and consumption in the carbon cycle

A

Microbes are involved in the production and consumption of methane (CH4). Methanogenic archaea produce methane in anaerobic environment, while methane-oxidizing bacteria consume it, converting it into CO2.

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12
Q

What was oparin and haldene’s hypothesis / what did the miller-urey experiment prove?

A

Organic molecules (amino acids) could form from inorganic molecules in earths reducing atmosphere spontaneously - due to high UV and lightning, eventually leading to cells

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13
Q

Cell theory

A

all living things are made of cells and all cells come from pre-exisiting cells

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14
Q

What are two things needed to be considered to be living

A
  1. be made of cells
  2. carry out life processes
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15
Q

Describe the shape of a eukaryotic cell and its structure

A
  • Round
  • Has a membrane-bound nucleus
  • Contain membrane-bound organelles
  • Larger and more structurally complex
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16
Q

Describe the shape of a prokaryotic cell and its structure

A
  • Skinny
  • Lacks a true nucleus
  • Lack membrane-bound organelles
  • Smaller and less complex
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17
Q

Define Microorganism

A

an organism that usually can only be seen with a microscope

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18
Q

Explain how microbes are cultured on nutrient agar

A
  • A control dish, kept unopened, checks for sterility.
  • Plates are taped partially closed to allow oxygen in, preventing harmful anaerobic bacteria from growing.
  • They are sealed to avoid contamination, as they contain millions of microbes after culturing and can cause illness if opened.
  • Plates are incubated upside down to prevent condensation from spreading microbes.
  • The incubation temperature is kept at or below 25°C to avoid growing human pathogens.
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19
Q

Describe how bacteria reproduce

A

Binary fission
1. The DNA copies itself
2. The cell membrane pinches the cytoplasm in half
3. Two identical bacterium form

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20
Q

Describe the bacterial structure

A

Slime capsule that protects the bacteria and prevents it dehydrating.

Cell wall helps to maintain the shape of the bacterium.

Cell membrane that controls the entry and exit of substances in and out of the bacterium.

Cytoplasm where cellular reaction occur.

Genetic material that controls the cellular reactions of the bacterium

Flagellum/a that allows the bacteria to move.

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21
Q

Describe bacteria and fungi nutrition (and where it happens in both bacteria and fungi)

A

The bacteria secrete enzymes onto their food source.

The enzymes chemically digest the food into smaller molecules outside the bacterium.

The bacteria then absorb the digested food.

Happens in the cell membrane in bacteria and the hyphae for fungi

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22
Q

Describe bacteria and fungi excretion

A

Bacteria and fungi excrete out the metabolic waste via passive diffusion.

Diffusion involves the process of movement of particles from a site of higher concentration to a site of lower concentration.

The waste material moves out through the cell membrane.

The diffusion of waste material can also occur against the concentration gradient, by active transport which uses energy.

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23
Q

Define respiration

A

The chemical breakdown of glucose to release energy for life processes

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24
Q

(BACTERIA) Compare anaerobic and aerobic respiration

A

AEROBIC:
- Oxygen required
- High amount of ATP energy made
- No damaging waste products

ANAEROBIC:
- No oxygen required
- Low amounts of ATP energy made
- Damaging waste product produced

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25
(BACTERIA) anaerobic and aerobic respiration equations
Aerobic: glucose + oxygen -> carbon dioxide + water Anaerobic: lactose + water -> lactic acid (Fermentation)
26
What conditions do bacteria need?
Bacteria need warmth, water, a food source, oxygen, space and a suitable pH.
27
Why does exponential growth for bacteria not continue?
- Food runs out - Space runs out - Toxic waste products build up
28
Describe the rhizopus fungus structure
Sporangium that are swellings at the tips of hyphae produce and contain spores. Spores that are small tough reproductive cells Hyphae that are fine threads that grow throughout a food source that carry out extracellular digestion. Mycelium (a network of hyphae).
29
Describe fungi reproduction
Certain hyphae grow upwards and produce swellings at their tips which are called sporangium. A large number of spores form in the sporangia. The sporangia burst releasing the spores into the air. The spores that land on moist food germinate and grow hyphae.
30
Describe anaerobic respiration for fungi + equation
Some fungi such as yeast complete their own version of anaerobic respiration called fermentation. glucose -> ethanol + carbon dioxide
31
Describe fermentation (yeast) in the context of making bread
Yeast, sugar and flour is left in a warm place. The yeast uses the sugar to respire excreting carbon dioxide and alcohol which causes the dough to rise. The alcohol evaporates during the baking process. The warmth increases the reaction rate.
32
Describe fermentation (yeast) in the context of making beer
Yeast and sugar is left in a warm place. The yeast uses the sugar to respire excreting carbon dioxide and alcohol which adds bubbles to the beer. The warmth increases the reaction rate.
33
Describe structure of viruses
Protein coast (capsid) to protect the genetic material. Genetic material that holds the instructions to replicate more viruses. Spike protein that allows these viruses to penetrate host cells.
34
Describe viral replication
A virus attaches themselves to the cell. The virus penetrates the cell membrane and injects its genetic material into the cell. The viral genetic material instructs the cell to construct viral protein coasts and genetic material. The new protein coats and genetic material are assembled into new viruses. This occurs until the cell bursts and the new viruses are released to attack more cells.
35
Why are viruses not considered living organisms?
Not composed of cells and they do not carry out the 7 life processes Do not respire or have any of their own metabolic processes. Therefore they do not excrete and do not need to feed as they do not require their own source of nutrition. Viruses also cannot reproduce independently and rely on host cells for replication. They also do not move themselves, sense or grow.
36
Define fermentation
a metabolic process in which microorganisms (bacteria, yeast or fungi) convert sugars and other organic compounds into various products
37
Describe fermentation (bacteria) in the context of cheese making
A starter culture of bacteria is added to warm milk. Curds are produced that are solid which are separated away from the whey (the remaining liquid). Bacteria and fungi are added to the curds to add different flavours of cheese as it matures.
38
Describe fermentation (bacteria) in the context of yoghurt making
A starter culture of bacteria is added to warm milk. The lactose in the milk is fermented by the bacteria to make lactic acid. The milk becomes a more viscous liquid in the process.
39
What is the gut microbiome?
A complex community of microorganisms
40
State 3 elements of the gut microbiome
Resides in the gastrointestinal tract, primarily the colon. Weighs about 2kg and is bigger than the average human brain. It contains at least 150 times more genes than the human genome.
41
Define decomposer
an organism that feeds on and breaks down dead plant or animal matter, making organic nutrients available to the ecosystem
42
Explain the process of sewage treatment
The sewage passes through screens to remove solids over 3mm, which are sent to landfill. In a sedimentation tank, sludge settles out and separates from the liquid. The liquid moves to an aeration tank, where aerobic bacteria break down contaminants, reducing nitrogen and phosphorus. Stones in the tank provide surface area for bacteria, and constant aeration keeps oxygen levels high to prevent anaerobic growth. The water is then treated with UV light to kill pathogens and released into the harbour. The sludge is sent to an anaerobic digester, where microbes produce methane (used for electricity) and CO₂. The leftover sludge is used as compost.
43
What do saprophytic bacteria do?
Saprophytic bacteria breakdown dead organic matter such as lawn clippings, leaves and food waste
44
Explain the process and conditions of compost heaps
Compost heaps should be watered to keep microbes moist and prevent fires, as microbial respiration generates heat. Turning the compost provides oxygen, supporting aerobic bacteria and reducing odors from anaerobic bacteria. Lime can be added if the compost is too acidic. Placing compost on soil allows worms to enter, helping to break down material and aerate the heap.
45
Define pathogen
disease causing microorganism
46
Give an example each of bacteria, fungi and viral pathogens
Tetanus, tinea (athletes foot), measles
47
How are pathogens spread
in contaminated water or food through the air by contact with a contaminated person (direct) or surfaces/objects (indirect) by bodily fluids by vector animals e.g. mosquitoes by transfer from animals to humans e.g. avian flu
48
How does bacterial food poisoning occur in the context of being left in warm temperatures?
Staphylococcus can multiply in the food. They release toxins into the food which causes food poisoning to develop quickly if consumed.
49
How does bacterial food poisoning occur in the context of not being cooked at high temperatures?
Campylobacter and Salmonella can cause food poisoning if the food is not cooked because cooking at high temperatures kills these microbes.
50
What is listeria and who is dangerous to?
Bacteria that multiplies in the body and is dangerous for those with low immunity
51
What can listeria be found in?
Fish, soft cheeses, deli meats
52
What are some safety rules to prevent food poisoning?
Hands should be washed thoroughly with soap and warm water to remove microbes and prevent the contamination of food. Food must be stored at the correct temperature (usually under 4oC) to prevent microbes multiplying and toxins building up. Food must be stored in sealed containers so raw meat juices cannot cross contaminate other foods. Different chopping boards and knives should be used on raw meats and other foods to prevent cross contamination. Food must be cooked thoroughly to kill microbes that can cause food poisoning.
53
What does high temperature in food preservation do?
All micro-organisms killed
54
What does pasteurisation in food preservation do?
Most micro-organisms killed
55
What does refrigeration and freezing in food preservation do?
Slows growth of microorganisms
56
What does drying in food preservation do?
prevents growth of microorganisms by lack of water
57
What does sugaring/salting in food preservation do?
prevents growth of microorganisms by lack of water (dehydration)
58
What does pH in food preservation do?
prevents growth of microorganisms by low pH
59
What does oxygen in food preservation do?
prevents growth of microorganisms by no oxygen
60
What does irradiation in food preservation do?
kills all microorganisms
61
what does chemicals in food preservation do?
Kills all microorganisms
62
Describe soap and its purpose
A fat-based cleaner that will remove most microbes from the surface of the skin.
63
Describe antiseptics and its purpose
Diluted chemicals which stio bacteria reproduction but they are NOT harmful to skin cells so they can be applied to cuts and prior to surgical incisions.
64
Describe disinfectants and its purpose
Chemicals that inhibit bacteria reproduction (stop bacteria reproducing) but they are harmful to skin cells so they are used in household cleaning products
65
Define antibiotics
Antibiotics are chemicals produced by fungi that kill or inhibit the reproduction and growth of bacteria.
66
How do vaccines work?
Vaccines introduce a small amount of a pathogen to the body. Enough for the body to recognise but not enough to cause illness. This means the body can produce antibodies against the disease and form memory cells. If the pathogen is infected with the pathogen again, the body has memory cells to respond quickly and is able to produce the right antibody quick to specifically attack the virus. Vaccines work to boost your immunity to a disease.
67
Why are viruses still a thing?
Viruses are able to replicate extremely quickly. This means they can mutate quickly as well. As viruses spread and mutate, they can become different strains that the immune system no longer recognises. This new strain is then able to spread and infect people.
68
Describe a tetanus shot
A tetanus shot delivers antibodies directly, however, these have a short life span and only last around 10 years so you need to get boosters.
69
Describe your immune system (what do they do)
Your immune system is constantly working to keep pathogens at bay and keep you healthy.
70
How does antibiotic resistance occur
High number of bacteria. A few of them are resistant to antibiotics. Antibiotics kill bacteria causing the illness, as well as good bacteria protecting the body from infection. The resistant bacteria now have preferred conditions to grow and take over. Bacteria can even transfer their drug-resistance to other bacteria, causing more problems.
71
Why is it important to finish all courses of antibiotics?
To ensure that all bacteria are killed. If the course is unfinished a few bacteria may survive and have a resistance.