Bio-Chem: Beers Law

Question 1

What’s Beer’s Law?

Answer 1

A=εbc

Question 2

What does A mean?

Answer 2

Absorbance

Question 3

What does ε mean?

Answer 3

Constant

Question 4

What does b mean?

Answer 4

Distance

Question 5

What does c mean?

Answer 5

Concentration

Question 6

A device used to measure the color transmitted from a chemical solution

Answer 6

Spectrophotometer

Question 7

What equation is used to calculate absorbance from transmittance?

Answer 7

A = 2-log(% Transmittence)

Question 8

Why do you need a blank solution when using a spectrophotometer?

Answer 8

It’s the base solution you are adding to it, making that solution 0,0

Question 9

What is transmittance?

Answer 9

The colors that pass through an object

Question 10

What is absorbance?

Answer 10

The color absorbed by an object

Question 11

What does a high ε tell you?

Answer 11

A chemical is effective at absorbing light

Question 12

How does the path length (b) affect absorbance?

Answer 12

The longer the path, the more light wil be absorbed.

Question 13

If a 1.0 M solution has an absorbance of 0.50, what is the equation you would use to solve this?

Answer 13

0.50 = ε*1.0