Bio Lab Practical

Question 1

Arm:

Answer 1

Supports and connects the eyepiece lens to the base, Microscope should be held here.

Question 2

Base

Answer 2

Supports the Microscope

Question 3

Coarse Focus Knob

Answer 3

Used to move stage up and down (Using with scanning objective)

Question 4

Condenser

Answer 4

Used to focus light on Specimen (Found Beneath the stage )

Question 5

Eyepiece/Ocular Lens

Answer 5

Ocular lens 10x magnification

Question 6

Fine Focus Knob

Answer 6

Used to adjust the specimen into final focus

Question 7

Light source

Answer 7

Serves as source of light for the microscope

Question 8

Scanning Objective

Answer 8

Shortest Objective , Magnifies 4x , ALWAYS START FIRST

Question 9

Low Power Objective

Answer 9

Used for coarse and preliminary focusing magnifies 10x

Question 10

High Power Objective

Answer 10

Used for final focusing , Magnifies 40x

Question 11

Stage

Answer 11

Platforms on Which slides are placed , Knobs can move stage up down or side to side

Question 12

Stage Clips

Answer 12

Secures the slides

Question 13

Monocular Microscope

Answer 13

A microscope with one ocular

Question 14

Binocular Microscope

Answer 14

A microscope with two oculars

Question 15

Resolution is…

Answer 15

The smallest distance between two points on a specimen that can still be distinguished as two separate entities

Question 16

Magnification is..

Answer 16

Getting closer to the specimen/magnifying the view. This indicates how many times the object you see has been enlarged

Question 17

Protein=

Answer 17

Biuret Reagent

Question 18

Simple sugars=

Answer 18

Benedict solution

Question 19

Starch=

Answer 19

Lugols Solution

Question 20

Positive control is

Answer 20

Uses a treatment that is known to influence the results(achieves results similar to hypothesis)

Question 21

Negative Control is

Answer 21

Uses a treatment that is know NOT to influence the results (given a placebo or no treatment at all)

Question 22

What does Gel electrophoresis do?

Answer 22

It separates the DNA and makes it easier to analyze based on size and charge.

Question 23

What are micropipettes used for?

Answer 23

to transfer volumes of samples into the well in the gel

Question 24

what is Electrophoresis?

Answer 24

a technique used to separate dna,rna or protein based on their size and electrical charge. The electric current is used to make molecules move throughout the gel

Question 25

What is the outcome of using Electrophoresis. gel?

Answer 25

we get to see how many different dna fragments are present in a sample and how large they are relative to one another

Question 26

What does Size Exclusion Chromatography do?

Answer 26

Separates molecules by filtration through a gel

Question 27

What does paper chromatography do

Answer 27

The solvent carries the dissolved pigment as it moves up the paper

Question 28

What happens in Strawberry lab

Answer 28

the alcohol pushes the dna out of the liquid while the rest of the liquid remained in the solution. there was a white/gooey clear dna strands in the alcohol layer and the 2 layers. the dna clumped in this activity making it easier for us to see the single strands of dna

Question 29

Dihybrid Cross is

Answer 29

a cross between 2 organisms with both being heterozygous for 2 different traits

Question 30

Pedigree is

Answer 30

a chart that diagrams the inheritance of a trait or condition through generations of a family

Question 31

what is the General importance of model organisms?

Answer 31

it provides valuable insight into biological systems at the cell, tissue, organ or system level. BRASSICA RAPA= they do not self pollinate, they are genetically diverse . it can be informative in research of disease

Question 32

In cold water , the food coloring

Answer 32

spreads out throughout the water SLOWLY

Question 33

In hot water , the food coloring

Answer 33

spreads out and diffuses faster . at high temperatures particles move faster. This faster movement allows diffusion to occur more quickly

Question 34

Chemical changes to strawberry

Answer 34

Ethanol-repeated the dna salt-draws out the liquid

Question 35

Dialysis Tubing

Answer 35

Starch Solution in the tube turned colors because the lugols solution was not able to pass through . The starch molecules are too big to exit through the tube