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microbiology lab > Biochemical Test Part 1 > Flashcards

Biochemical Test Part 1 Flashcards

(45 cards)

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1
Q

Exp 22A Starch hydrolysis

substrate and producs

A

starch—–glycoses

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2
Q

Exp 22B- Lipid hydrolysis

substrate and products

A

tributyrin—-glycerol and fatty acids

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3
Q

Exp 25A Indole production
substrate and products
combined with

A

triptophan—-indole + pyruvic acid+ammonia

combined with 26

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4
Q

Exp 26 Hydrogen sulfate test
substrate and products
combined with

A

thiosulfate—–sulfite+hydrogen sulfide gas

combined with 25 A

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5
Q

Exp 25 B methyl red test
substrate and products
combined with

A

glucose—acid

25 C

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6
Q

Exp 25 C Voges-Prokauer test
substrate and products
combined with

A

glucose—base

25 B

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7
Q

Exp 25 D Citrate Utilization test

substrate and products

A

citrate—pyruvic acid+acetic acid+CO2

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8
Q

Exp 27 Urease test

substrate and products

A

urea—CO2+H20+ammonia

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9
Q

Exp 23 Carbohydrate Fermentation test

substrate and products

A

carb—Acid (A) or Acid gas (AG)

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10
Q

Exp 28 Litmus milk reaction test

substrate and products

A

milk—-7 possible results

  • Lactose fermentation
  • Gas production
  • Litmus reduction
  • curd formation
  • proteolysis
  • alkaline reaction
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11
Q

Starch hydrolysis

+ and -

A

+ remains light

- dark

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12
Q

Lipid Hydrolysis

+ and -

A

+ media around becomes transparent

- media stays cloudy

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13
Q 
Indole production (25 A) combined with 26 
\+ and -
A

+ Red layer on surface of media

- No red layer in media

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14
Q

hydrogen sulfate test (26) combined with 25 A
this is done first!!
+ and -

A

+ black in media

-no black in media

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15
Q

methyl red test (25 B) combined with 25 C

+ and -

A

+ any red present in media

- no red

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16
Q

Voges- Prokauer test (25 C) combined with 25 B

+ and -

A

+ and red in media

- no red in media

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17
Q

Citrate utilization test (25 D)

+ and -

A

+ any blue in media

-no blue in media

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18
Q

Urea (27)

+ and -

A

+ pink (metallic)

- peachy

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19
Q

Exp 29- Nitrate reduction test

substrate and products

Study These Flashcards
A

nitrate—-nitrites—ammonia

20
Q

Exp 30 Catalase test

substrate and product

Study These Flashcards
A

hydrogen peroxide (H2O2)—H20 and O2

21
Q

Exp 29- Nitrate reduction test

substrate and products

Study These Flashcards
A

nitrate—-nitrites—ammonia

22
Q

Exp 30 Catalase test

substrate and product

Study These Flashcards
A

hydrogen peroxide (H2O2)—H20 and O2

23
Q

Media used in exp 14: media for routine cultivation

Study These Flashcards
A

Broths of salt, dextrose, nutrient and yeast extract

24
Q

Media used in temp requirement

Study These Flashcards
A

Trypticase soy agar plates

25
Media used for oxygen requirement
Brain heart melt tubes and SAB melt tube
26
Starch hydrolysis media
Starch plate
27
Lipid hydrolysis media
Tributyrin plate
28
Carb fermentation test media
Broths of lactose, dextrose and sucrose
29
Indole production test media
SIM deep
30
Methyl red test and voges media
MRVP broths
31
Citrate media
Citrate slants
32
Urea media
Urea broths
33
Nitrate media
Try process nitrate broths
34
Catalase test media
Trypticase soy slant
35
Lactose Fermentation acid
media is pink | broke down lactose into acid
36
acid followed by reduction
majority white with pink band on top | lactose---acid---reduced acid
37
acid reduction and curd
solid white at bottom, white broth middle and pink band on top. Lactose --acid--reduced acid casein--curd
38
Acid reduction, curd and gas
solid bottom white, white broth in middle with fissures and pink on top casein---curd lactose---acid and gas acid---reduced acid
39
litmus reduction
white media with purple band on top litmus--reduced received electrons DIDNT break down anything (attached form of hydrogen)
40
proteolysis
whey like brownish with deep purple band | casein---aminoacids
41
Alkaline reaction
media unchanged or deep blue | lactose---base
42
Nitrate reduction test +, -
any red in media----POSITIVE NITRITE red in media after zinc----NEGATIVE, NITRATE (product) no red in media after zinc---POSITIVE AMONIA
43
Iodine Reagent
Starch hydrolysis
44
Kovac's
Indole production reagent
45
hydrogen peroxide
Catalse test reagent

microbiology lab (9 decks)

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