Biochemistry Lab Flashcards
(21 cards)
In the anaerobic glycolysis experiment, the optical density of the sample was measured as 100 at 420 nm wavelength, and the optical density of the standard was measured as 26.4. Accordingly, which of the following is the glucose concentration found in the sample? (Standard concentration (Cstd): 0.088, Dilution factor (F): 300)
a) 200
b) 400
c) 500
d) 100
e) 300
d) 100
Which of the following is the method used for qualitative determination of glucose in urine in the glucose tolerance test?
a) Glucose oxidase
b) Millon
c) Edman
d) Benedict
e) Kjeldahl
d) Benedict
In anaerobic glycolysis experiment: The colored compound obtained in the determination of phosphorus is measured spectrophotometrically in which of the following wavelengths.
a) 560
b) 410
c) 600
d) 340
e) 520
c) 600
Which of the following is the reagent used for the formation of yellow colored product in the qualitative determination of lactic acid in the anaerobic glycolysis experiment?
a) Ba(OH)2
b) FeCl3
c) MgCl₂
d) ZnSO4
e) CuSO4
b) FeCl3
Which of the following is the principle of Folin-Wu method used in determination of serum glucose in anaerobic glycolysis experiment?
a) Glucose forms a purple colored compound FeCl3
b) Glucose creates a pinkish color with copper sulphate in a basic environment
c) Glucose reduces Cu+2 to Cu+1 in an alkaline environment
d) Glucose forms a white colored precipitate with Ba(OH)2
e) Glucose forms yellow compound with ammonium molybdate
c) Glucose reduces Cu+2 to Cu+1 in an alkaline environment
Which of the following is the diagnostic criteria for diabetes mellitus?
a) Fasting Plasma se ≥ 100mg / dL
b) In Oral Glucose Tolerance Test (OGTT), 2nd hour plasma glucose level ≥ 140 mg/dL
c) Randomly measured blood se ≥ 200mg / dL and accompanied by symptoms of diabetes mellutus
d) Presence of ketone bodies in urine
e) HbA1c level ≥ 5.4%
c) Randomly measured blood se ≥ 200mg / dL and accompanied by symptoms of diabetes mellutus
kan şekeri diyagnoz ve takibinde kullanılan teknik?
HbA1c
sonucu yanlış çıkan deneyde hangi aşamada yanlış yapmış olmalıyız?
the test tube was not pipetted with alkaline CuSO4
kalıntılarını uzaklaştırmak için kulandığımız madde?
Ba(OH)2
deneyin sonunda tüpte hangi ürünlerin oluşması beklenmez?
Atp
Amp and pi
Mg and k
Pyruvate
Pyruvate
anaerobic deneyini inhibe etmek için hangisi kullanılır?
Mg
K
Ba(OH)2
CuSO4
Sulfiric acid
Ba(OH)2
hangisi 2 saatin sonunda diabet teşhisi koymak için sınır kriterdir?
126
140
180
200
200>
200>
hangisi doğru?
-lactic acid forms a yellow iron lactate with fecl3
..
hangist yanlış?
- The blue color that occurs in the urine glucose assay indicates the high glucose concentration
..
LAB sorusu,
- Coloring step was done with ANSA.
,,
anaerobic glikolizle ilgili hangisi yanlıştır?
- The amount of glucose in 60-minute tube is greater than in the 0-minute tube.
..
hangiler doğrudur?
II- Blood glucose determination is performed according to the Folin-wu method.
IV- Acetone determination in urine is done qualitatively.
..
What is the principle of Folin-Wu method for determination of blood glucose?
A) Of Cu+1to Cu+2 reduction of glucose in alkaline environment
B) glucose increase with NaOH
C) in the acidic environment, glucose of Cu+click to Cu+³ oxidation
D) boiling up to the boiling point of glucose
E) precipitation with heavy metals
A) Of Cu+1to Cu+2 reduction of glucose in alkaline environment
Which method is used for the determination of glucose concentration in the blood, reduced copper and phosphomolybdic acid colored complex formed?
A) Folin-Wu
B) Salivanof
C) Edman
D) Benedikct
E) Biüret
A) Folin-Wu
Which is used for the determination of glucose in the urine test?
A) Folin-Wu
B) Biüret
C) Ninhidrin
D) Sanger
E) Benedict
E) Benedict
The patient to make a diagnosis of pre-diabetes, OGTT glucose concentration for the second hour after glucose administration should be how?
A) 150-220 mg/dL
B) 100-120 mg/dL
C) 200-240 mg/dL
D) 140-199 mg/dL
E) 80-100 mg/dL
D) 140-199 mg/dL
