A form of TSE that affects young males and females?
vCJD
What composes the primary structure of proteins?
Amino acids
Gene responsible for Prion susceptibility
PRNP
A higher order of protein structure composed of multiple domains
Tertiary structure
Protein in RBCs that gives its characteristic pinkish tint
Heme
Histoblot technique for Lipids
Fareast blot
Beta sheets that run towards opposite directions
Anti parallel
Y/N: can proteins renature after being denatured by Sodium Dodecylsulfate
No
Quaternary structure is also called
Polypeptides
What histoblot technique will you use if you want to check if a person is susceptible to prion-related diseases?
Northern blot
Composition of PrPc?
3 alpha helices and 2 beta sheets
Inherited TSEs are in what type of heredity?
Autosomal dominant
Chromosome where the PRNP gene is located
C20
The most common form of an alpha helix
Right handed
Plot that measures the prevalence of alpha helices and beta sheets
Ramachandran plot
Histoblot technique that detects proteins
Western blot
What type of pairing of codon 129 of PRNP gene that makes susceptibility to prion disease increase
Homozygosity
Heterozygosity of codon 129 gives resistance to prion disease. T/F
True
Defective proteins in the cell is tagged for degradation by…
Ubiquitin
Was prevalent in Europe that made TSEs a worldwide topic
Mad cow’s disease / BSE
Who coined the term prion
Prusiner
Leucine zipper is what type of protein structure?
Secondary super structure
What makes secondary super structures?
Loops and bends
Histoblot technique that detects DNA
Southern blot
The result of translation
Amino acids
What process is the conversion of PrPc to PrPsc called?
Post translational modifications
T/F: all proteins have a quaternary structure
False
Mutations to genes will affect what protein structure initially?
Primary
Changes in secondary or tertiary structure affect its function. T/F
True
PrPsc conversion is reversible. T/F
True
PrPc conversion normally occurs in humans. T/F
True
Where does protein synthesis occur?
RER and ribosomes
The structure of integral proteins
Helix
Major composition of the cell membrane
Proteins
Globular proteins is abundant of what type of secondary structure?
Beta sheet
Functional units that make up the tertiary structure
Domains
Are all enzymes proteins?
No
Are all proteins enzymes?
No
T/F: prion disease is due to primary structure alteration
False
T/F: prion is a quaternary structure
False
Can proteins be broken down to its component amino acids? Y/N
Yes
Prion disease was first observed in…
Papua New Guinea
TSE stands for?
Transmissible Spongiform Encelopathy
Are all encelopathies due to prions?
No
Are prion and related disease curable?
No
Biopsy of what structure can you detect PrPsc among young people with vCJD?
Tonsil
What is the central dogma
Replication>transcription>translation
Proteins is an essential part of our diet? Y/N
Yes
Protein structure deformity of the globulin group of hemoglobin will result to…
Anemia
Prion is a shorter term for….
Proteinaceous infectious agent