Biochemistry Proteins/Prions

Question 0

A form of TSE that affects young males and females?

Answer 0

vCJD

Question 1

What composes the primary structure of proteins?

Answer 1

Amino acids

Question 2

Gene responsible for Prion susceptibility

Answer 2

PRNP

Question 3

A higher order of protein structure composed of multiple domains

Answer 3

Tertiary structure

Question 4

Protein in RBCs that gives its characteristic pinkish tint

Answer 4

Heme

Question 5

Histoblot technique for Lipids

Answer 5

Fareast blot

Question 6

Beta sheets that run towards opposite directions

Answer 6

Anti parallel

Question 7

Y/N: can proteins renature after being denatured by Sodium Dodecylsulfate

Answer 7

No

Question 8

Quaternary structure is also called

Answer 8

Polypeptides

Question 9

What histoblot technique will you use if you want to check if a person is susceptible to prion-related diseases?

Answer 9

Northern blot

Question 10

Composition of PrPc?

Answer 10

3 alpha helices and 2 beta sheets

Question 11

Inherited TSEs are in what type of heredity?

Answer 11

Autosomal dominant

Question 12

Chromosome where the PRNP gene is located

Answer 12

C20

Question 13

The most common form of an alpha helix

Answer 13

Right handed

Question 14

Plot that measures the prevalence of alpha helices and beta sheets

Answer 14

Ramachandran plot

Question 15

Histoblot technique that detects proteins

Answer 15

Western blot

Question 16

What type of pairing of codon 129 of PRNP gene that makes susceptibility to prion disease increase

Answer 16

Homozygosity

Question 17

Heterozygosity of codon 129 gives resistance to prion disease. T/F

Answer 17

True

Question 18

Defective proteins in the cell is tagged for degradation by…

Answer 18

Ubiquitin

Question 19

Was prevalent in Europe that made TSEs a worldwide topic

Answer 19

Mad cow’s disease / BSE

Question 20

Who coined the term prion

Answer 20

Prusiner

Question 21

Leucine zipper is what type of protein structure?

Answer 21

Secondary super structure

Question 22

What makes secondary super structures?

Answer 22

Loops and bends

Question 23

Histoblot technique that detects DNA

Answer 23

Southern blot

Question 24

The result of translation

Answer 24

Amino acids

Question 25

What process is the conversion of PrPc to PrPsc called?

Answer 25

Post translational modifications

Question 26

T/F: all proteins have a quaternary structure

Answer 26

False

Question 27

Mutations to genes will affect what protein structure initially?

Answer 27

Primary

Question 28

Changes in secondary or tertiary structure affect its function. T/F

Answer 28

True

Question 29

PrPsc conversion is reversible. T/F

Answer 29

True

Question 30

PrPc conversion normally occurs in humans. T/F

Answer 30

True

Question 31

Where does protein synthesis occur?

Answer 31

RER and ribosomes

Question 32

The structure of integral proteins

Answer 32

Helix

Question 33

Major composition of the cell membrane

Answer 33

Proteins

Question 34

Globular proteins is abundant of what type of secondary structure?

Answer 34

Beta sheet

Question 35

Functional units that make up the tertiary structure

Answer 35

Domains

Question 36

Are all enzymes proteins?

Answer 36

No

Question 37

Are all proteins enzymes?

Answer 37

No

Question 38

T/F: prion disease is due to primary structure alteration

Answer 38

False

Question 39

T/F: prion is a quaternary structure

Answer 39

False

Question 40

Can proteins be broken down to its component amino acids? Y/N

Answer 40

Yes

Question 41

Prion disease was first observed in...

Answer 41

Papua New Guinea

Question 42

TSE stands for?

Answer 42

Transmissible Spongiform Encelopathy

Question 43

Are all encelopathies due to prions?

Answer 43

No

Question 44

Are prion and related disease curable?

Answer 44

No

Question 45

Biopsy of what structure can you detect PrPsc among young people with vCJD?

Answer 45

Tonsil

Question 46

What is the central dogma

Answer 46

Replication>transcription>translation

Question 47

Proteins is an essential part of our diet? Y/N

Answer 47

Yes

Question 48

Protein structure deformity of the globulin group of hemoglobin will result to...

Answer 48

Anemia

Question 49

Prion is a shorter term for....

Answer 49

Proteinaceous infectious agent