Biological Molecules Flashcards
(14 cards)
Carbohydrates
Maltose -> Starch
Proteins
Long chains of amino acids -> proteins
Lipids
Glycerol and fatty acids -> lipids
Food tests
Glucose: Benedict solution
Starch: Iodine solution
Protein: Biuret solution
Iodine: Sudan (III) Test
Glucose
Use benedict solution
blue to green or yellow in low conc
blue - brick red in high conc
Starch
Iodine solution
blue to browny orange or black
Proteins
Use Biuret solution
blue to pink or purple
Lipids
Use Sudan (III) Test
stains lipids (top layer will be brick red)
Role of enzymes
Enzymes act as biological catalysts. Speed up the rate of reaction without being used up.
It is necessary for all organisms as they maintain reaction speeds in metabollic reactions
Mechanism of enzyme action
Specific to one particular substrate as the active site of the enzyme, where the substrate attaches to its complementary shape.
‘lock and key’
Temperature and Enzyme Function
Enzymes are proteins and have a specific shape, determined by the amino acids that build them and held in place by bonds. This is important as it ensures which substrate will fit into the active site.
Enzymes work fastest at their optimum temperature.
Enzymes denature when temperatures are too high.
Practical Investigating temp and enzyme activity
X
pH & Enzyme function
Optimum pH for most enzymes is 7
if pH is too high or low the bonds that hold the amino acids together will be disrupted. Substrate no longer fits into the active side reducing rate of activity.
CORMMS
C - Change
O - Organism
R - Repeat
M - Measurement 1
M - Measurement 2
S - Same (control)