BIOSAFETY AND BIO CONTAINMENT

Question 1

Any microbiological entity, cellular or non-cellular,
naturally occurring or engineered, capable of
replication or of transferring genetic material that may
be able to provoke infection, allergy, toxicity, or other
adverse effects in humans, animals, or plants

Answer 1

BIOLOGICAL AGENTS

Question 2

Bacteria, viruses, fungi, parasites, prions, and protozoa

Answer 2

MICROORGANISMS

Question 3

Produced by biological agents

Answer 3

TOXINS

Question 4

Created through genetic
engineering

Answer 4

GENETICALLY MODIFIED ORGANISMS

Question 5

Derived from humans, animals, or insects

Answer 5

CELL CULTURE

Question 6

Such as animal dander, pollen, urine,
saliva, or sap

Answer 6

PRODUCTS OF BIOLOGICAL AGENTS

Question 7

Used in research

Answer 7

ANIMALS, ARTHROPODS, PLANTS

Question 8

Any material comprised of, containing, or that may contain biological
agents and/or their harmful products, such as toxins and allergens.

Answer 8

BIOLOGICAL MATERIAL

Question 9

EXAMPLE BIOLOGICAL MATERIAL

Answer 9

1. Blood
2. Tissue
3. Proteins
4. Bacteria, viruses and fungi
5. Toxins

Question 10

Potential source of harm caused by biological
materials.

Answer 10

BIOHAZARD

Question 11

Containment principles, technologies, and
implemented to practices unintentional
pathogens and toxins, or their unintentional
prevent exposure to release.

Answer 11

LABORATORY BIOSAFETY

Question 12

PURPOSE OF LABORATORY SAFETY

Answer 12

Protect users
* Protect those outside the laboratory
* Protect the environment

Question 13

*Institutional and personal security measures
designed to prevent the loss, theft, misuse,
diversion, or intentional release of pathogens
and toxins

Answer 13

LABORATORY BIOSECURITY

Question 14

Protecting people from bad bugs.

Answer 14

BIOSAFETY

Question 15

Protecting bad bugs from bad
people.

Answer 15

BIOSECURITY

Question 16

principle of biosafety

Answer 16

1. Practice and procedures
   * Standard practices
   * Special practices and considerations
2. Safety equipment
3. Facility design and construction
4. Increasing levels of protection

Question 17

Most important concept/strict
adherence

Answer 17

Standard Microbiological Practices

Question 18

Physical containment of highly pathogenic organisms or
agents usually by isolation in environmentally and
biologically secure cabinets or rooms to prevent accidental
infection of workers or release into the surrounding
community, especially during scientific research

Answer 18

BIO CONTAINMENT

Question 19

control hazard at the source

Answer 19

PRIMARY BARRIER/CONTAINMENT

Question 20

structure surrounding primary barrier

Answer 20

SECONDARY BARRIER/CONTAINMENT

Question 21

Secondary barrier/engineering
controls
* Contributes to worker protection
* Protects outside the laboratory

Answer 21

FACILITY DESIGN AND CONSTRUCTION

Question 22

Increasing levels of employee and
environmental protection

Answer 22

Biosafety Levels 1-4 (BSL)

Question 23

Laboratory animal facilities
* Plant, GMO facilities
* Animal models that support research

Answer 23

Animal Biosafety Levels 1- 4 (ABSL) and
Agricultural (BSL-3-Ag)

Question 24

Combination of laboratory practicesand
procedures, safety equipment (primary
barriers)and laboratory facilities (secondary
barriers)

Answer 24

BIOSAFETY LEVEL

Question 25

other term for biosafety level

Answer 25

Containment Levels

Question 26

Well characterized, non-pathogenic organisms or agents * Open bench - no containment * Use good laboratory practices, waste disposal, and aseptic techniques

Answer 26

BSL 1

Question 27

Builds upon BSL-1 * Agents of moderate hazard to personnel or environment * Examples: most non-respiratory, non lethal, agents * Basic lab, but restricted access * Containment during certain processes (i.e. aerosols, large volumes, etc.) * Autoclave and Biological Safety Cabinet desired * Use good laboratory practices, waste disposal, and aseptic techniques

Answer 27

BSL 2

Question 28

Used to describe laboratories where work with microorganisms is conducted in a BSL-2 laboratory with biosafety practices and procedures typically found at BSL-3 * NOT a recognized containment level in biosafety guidance documents

Answer 28

BSL 2+

Question 29

High containment * Agents of high hazard to personnel or environment * Agents that may cause serious or potentially lethal disease through the inhalation route of exposure * Clinical, diagnostic, teaching, research, or production facilities where work is performed with indigenous or exotic agents

Answer 29

BSL 3

Question 30

Maximum containment * Dangerous and exotic agents that pose a high individual risk of aerosol transmitted laboratory infections * Total containment, airtight labs, “submarine” doors, air pumps, water treatment, HEPA filtration, etc. * Positive pressure “moonsuits”

Answer 30

BSL 4

Question 31

Assignment of microorganisms into groups based on: * Pathogenicity of the organism * Mode of transmission and host range * Local availability of effective preventative measures * Local availability of effective treatment * Pathogen Safety Data Sheets (PSDS)

Answer 31

RISK GROUP

Question 32

are infections, either symptomatic or asymptomatic, that are acquired through laboratory or laboratory- related activities, as a result of working with infectious agents

Answer 32

LABORATORY ACQUIRED INFECTION

Question 33

was first used to clone DNA segments in bacterial hosts in order to overexpress specific gene products for further studies

Answer 33

Recombinant DNA Technology (Genetic Engineering)