Biuret Flashcards
1
Q
At what temperature does buret compound form from 2 urea
A
180°c
2
Q
What makes purple color of biuret assay?
A
Cupric peptide complex
3
Q
Max wavelength absorbance of cupric peptide complex?
A
540nm
4
Q
Absorbance of standard protein solution?
A
~0.5
5
Q
Absorbance for protein sample A?
A
0.341
6
Q
Absorbance for protein sample B?
A
0.201
