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MLS(ASCP) - Blood Bank > Blood Bank > Flashcards

Blood Bank Flashcards

(52 cards)

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1
Q

At what temperature is PF24 or FFP thawed for transfusion?

A

37oC

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2
Q

What is the expiration date of liquid plasma?

A

5 days after expiration of the whole blood unit from which it was collected

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3
Q

What is the expiration of irradiated pRBCs?

A

28 days or original expiration, whichever occurs first.

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4
Q

What is the expiration of frozen red cells?

A

10 years

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5
Q

At what temperature are granulocytes stored?

A

20 - 24oC

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6
Q

What is the blood donation deferral period for receiving measles, mumps, or yellow fever vaccine?

A

2 weeks

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7
Q

What is the blood donation deferral period for receiving a tattoo?

A

12 months

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8
Q

What is the blood donation deferral period for receiving a stem cell transplant?

A

12 months

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9
Q

What is the minimum hemoglobin/hematocrit required to donate whole blood?

A

Hemoglobin = > 12.5 g/dL

Hematocrit = > 38%

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10
Q

What is the blood donation deferral period for a female who had a sexual encounter with a male who had sexual contact with another male?

A

12 months

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11
Q

What is the blood donation deferral for persons who was/currently diagnosed with leukemia?

A

Indefinite deferral

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12
Q

What is the minimum weight required for whole blood donation?

A

110 lbs

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13
Q

How long must donor records be retained by the collection facility?

A

10 years

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14
Q

Refer to the illustration below.

Can you identify the inheritance pattern of this pedigree?

A

Autosomal Dominant

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15
Q

Refer to the illustration below.

Can you identify the inheritance pattern of this pedigree?

A

Autosomal Recessive

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16
Q

Refer to the illustration below.

Can you identify the inheritance pattern of this pedigree?

A

X-linked Dominant

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17
Q

Refer to the illustration below.

Can you identify the inheritance pattern of this pedigree?

A

X-linked Recessive

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18
Q

What lectin is used to agglutinate A1 or A1B cells?

A

Dolichos biflorus

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19
Q

Which lectin is used to agglutinate Group O red cells?

A

Ulex europaeus

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20
Q

Which lectin is used to agglutinate Group B cells?

A

Bandeiraea simplicifolia

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21
Q

What is the Rh phenotype of an individual who inherits genotype R1r?

22
Q

What is the Rh phenotype of an individual who inherits genotype R2R2?

Study These Flashcards
A

See table below:

23
Q

What is the most probable genotype using the Weiner nomenclature for the following phenotype illustrated below:

24
Q

What is the most probable genotype using the Weiner nomenclature for the following phenotype illustrated below:

25
What is the most probable genotype using the Rosenfield nomenclature for the following phenotype illustrated below:
Rh: 1, 2, -3, -4, 5
26
What is the most probable genotype using the Rosenfield nomenclature for the following phenotype illustrated below:
Rh: 1, -2, -3, 4, 5
27
What is the most probable genotype using the Fisher-Race nomenclature for the following phenotype: R2r
cDE/ce
28
If transfused, which Rh antibody(ies) would you expect a Ror individual to produce?
* Anti-C * Anti-E
29
What test is performed to calculate the amount of fetal red cells entering the maternal circulation during a hemorrhage event?
Kleihaeur-Betke acid elution test
30
How much whole blood and/or fetal red cells will a standard dose of Rh Immune globulin cover?
15 mL of packed cells or 30 mL of whole blood
31
List three ways in which you can inherit the Lu(a-b-) phenotype:
1. Homozygous inheritance of the recessive *LuLu* gene (Recessive) 2. Inheritance of an X-linked recessive gene *GATA-1* (X-linked recessive) 3. Inheritance of a mutated, dominant *KLFI* gene (Dominant inhibitor)
32
List 2 instances when a Dweak test is performed:
1. All donors who initial are immediate spin Rh negative 2. Rh negative pregnant females
33
What is the ABO Group of antibody screening cells?
Group O
34
Using the Weiner nomenclature, what is the Rh genotype of screening cells: SC-I, SC-II, and SC-III?
* SC-I = R1R1 * SC-II = R2R2 * SC-III = rr
35
When performing an Indirect Antiglobulin Test (IAT) antibody attachment is observed in which phases?
Immediate Spin (optional) 37oC AHG (antihuman globulin)
36
*Refer to the table below:* List 3 actions you could perform to resolve this ABO discrepancy:
1. Check the age of the patient 2. Incubate reverse group for 30 minutes (do not perform this on a newborn or cord blood sample) 3. Incubate reverse group at 4oC and incubate for 15 minutes. (Use screening cells as a control) Patient is probably Group O. The steps above will enhance plasma agglutination.
37
Refer to the table below: List 3 actions you could do to resolve this ABO discrepancy:
1. Acidify anti-B reagent to pH = 6 2. Run DAT 3. Run autocontrol Patient is most likely Group A. Reaction with anti-B is due to Acquired B-like antigen seen in patients with colorectal cancer or other GI tract conditions.
38
*Refer to the table below:* List 3 actions you could perform to resolve this ABO discrepancy:
1. Perform microscopic examination of the reverse group testing 2. Perform saline replacement technique 3. Perform IAT
39
*Refer to the table below.* How would you interpret this antibody screen?
Positive IAT
40
*Refer to the table below.* How would you interpret this antibody screen?
Invalid IAT Must repeat test
41
*Refer to the table below.* How would you interpret this antibody screen?
Positive IAT
42
*Refer to the table below.* How would you interpret this antibody screen?
Negative IAT
43
*Interpret the DAT panel below.* Should you perform an eluate on the sample?
DAT = Positive for complement only No eluate indicated
44
*Interpret the DAT panel below.* Should you perform an eluate on the sample?
DAT = Positive for IgG and complement Perform eluate
45
*Interpret the DAT panel below.* Should you perform an eluate on the sample?
Invalid - DAT panel should be repeated Do not perform eluate until poly AHG result is confirmed
46
List 3 blood group systems in which antigens are sensitive to papain/ficin treatment?
1. Duffy 2. MN 3. Xg
47
List at least 3 blood group systems in which antigens are resistant to papain/ficin treatment?
1. Rh 2. Kidd 3. S, s, U 4. Kell 5. Lewis 6. P 7. Lutheran
48
List 3 solutions used to enhance agglutination in IAT?
1. PEG 2. LISS 3. 22% Albumin
49
List 4 methods used to enhance antihuman globulin tests:
1. Increase incubation time 2. Increase temperature 3. Washing red cells 4. Increase serum:cell ratio
50
Donath-Landstiener antibody belongs to which blood group system?
P Blood Group
51
List 2 FDA approved methods used to automate blood bank serological testing:
1. Gel technology 2. Solid phase technology
52
How many vials of RhIg should a Rh(-) pregnant woman receive if the number of fetal cells determined by K-B test = 3.6%
7 vials of RhIg

MLS(ASCP) - Blood Bank (1 decks)

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