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Blood Bank Test Flashcards

(56 cards)

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1
Q

BB samples are routinely kept for ____ days total

A

10

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2
Q

Samples are OK to use for XM for ____ days from date of _______

A

3 days

collection

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3
Q

Samples must be kept in the BB for ____ days from date of transfusion

A

7

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4
Q

5 reasons to check patient’s history

A
  1. Look for past blood type - must match current
  2. Look at transfusion history (no trans? likely no Abs)
  3. Look for antibody history (clin sig? must honor it)
  4. Look at transfusion reaction history
  5. Look to see if any special requirements (CMV, IRR, Ag neg, freshness)
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5
Q 
What are the Ag frequencies of: 
D
C
E 
c 
e 
K
A 
D = 85
C = 70
E = 30
c = 80
e = 98
K = 9
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6
Q

Computer/Electronic XM:

- requirements

A
  1. Current Ab screen must be negative
  2. Must be no history of serum antibodies
  3. Two patient ABO/Rh types must be on file
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7
Q 
Coombs XM (full) 
- requirements
A

must perform in addition to the IS XM if there is

1. A history of or current unexpected clinically significant antibody

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8
Q

For FDA requirements for component prep, cGMP stands for what?

A

current good manufacturing practice

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9
Q

What is the primary goal of RBC preservation?

- that _____ of transfused cells should remain viable for _____

A

75%

24 hours

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10
Q

What biochemical changes happen when RBCs are stored:

  • K
  • NA
  • Hgb
  • ATP
  • 2,3 - DPG
  • pH
A
  • K: i
  • NA: d
  • Hgb: i
  • ATP: d
  • 2,3 - DPG: d
  • pH: d
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11
Q

How long do these preservative solutions allow blood to be stored:

  • ACD
  • CPD
  • CPDA-1
A

ACD: 21 days
CPD: 21 days
CPDA-1: 35 days
Adsol: 42 days

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12
Q

What is the process of making packed cells and platelet-poor plasma?

A

Do a hard spin on whole blood

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13
Q

What is the process of making platelet concentrate and platelet-poor plasma

A

Take whole blood and light spin then hard spin the platelet-rich plasma

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14
Q

What can be stored at 1-6C

A
  • Whole blood
  • packed red blood cells
  • leukocyte-reduced RBCs
  • washed RBCs
  • deglyc’s RBCs
  • thawed plasma
  • irradiated RBCs
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15
Q

What can be stored at room temp (22-24C)

A
  • Plateletpheresis platelets
  • random platelet concentrate
  • thawed pooled cryo
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16
Q

What can be stored at -18C

A
  • Frozen plasma products

- Frozen cryo

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17
Q

What can be stored at -65C

A
  • Frozen RBCs
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18
Q

Expiration 24 hours

A
  • Open systems
  • pooling products
  • washing
  • deglyc’d rbcs
  • thawed FFP
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19
Q

No change in expiration

A
  • closed system

- sterile docking

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20
Q

Expire in 10 years

A

frozen RBCs

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21
Q

Expire in 5 days

A
  • plateletpheresis platelets
  • random platelet concentrate
  • thawed plasma (PF24)
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22
Q

Original date or 28 days from date of irradiation whichever is sooner

A
  • Irradiation
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23
Q

Expire in one year

Study These Flashcards
A
  • frozen plasma

- frozen cryo

24
Q

Expires in 6 hours

Study These Flashcards
A
  • Single unit thawed cryo
25
Expires in 4 hours
- thawed and pooled cryo | - washed platelets
26
QC: | - Leukocyte-reduced RBC must have residual leukocyte content of ________
< 5x10^6
27
QC: | - Single Apheresis Donor platelet must contain ________ platelets
> or equal to 3.0x10^11
28
QC | - pH of plateletpheresis
> or equal to 6.2
29
QC - CRYO must contain ______ of Factor VIII - CRYO must contain ______ Fibrinogen
VIII: > or equal to 80 IU Fibrinogen: > or equal to 150mg
30
QC | - Platelet count of random platelet concentrate
> 5.5X10^10
31
QC | - max hematocrit of packed cells
< or equal to 80%
32
How do you ship Red cell products (liquid)
Wet ice or crushed ice, not touching donor units (separate by cardboard)
33
How do you ship Platelet products
Ship at room temp
34
How do you ship frozen products
ship with dry ice
35
Who gets Fresh Frozen Plasma?
- liver disease - coagulation deficiencies - DIC - TTP
36
Who needs CRYO?
- vWBD - Hemophilia A - XIII deficiencies - fibrinogen deficiencies - fibrin glue
37
Who gets platelets?
Thrombocytopenia
38
Who gets irradiated cellular components?
- prevent GVHD - Oncology Patients - Transplant Patients - Infant Patients - Directed Donor fm 1st degree family - IUT's - ITP - HLA matched platelets
39
Who gets packed red blood cells?
- Symptomatic anemia - Most surgery patients (massive trauma, BM, ECMO patients, PUBS, neonates)
40
Indications for RBC Transfusion (Normal Patient)
Hct less than 21% or Hgb Less than 7 g/dL
41
Indications for RBC Transfustion (coronary artery disease and unstable angina/myocardial infarction/cardiogenic shock)
Hct less than 24% or Hgb less than 8 g/dL
42
Indication or RBC Transfusion (Rapid blood loss)
> 30-40% of estimated blood volume not responding to appropriate volume resuscitation, or with ongoing blood loss
43
_____ Hgb almost always indicates transfusion | _____ Hgb rarely indicates transfusion
6g/dl | 10g/dl
44
One unit of packed RBCs raises the Hct how much?
3-5%
45
One unit of packed RBCs raises the Hgb how much?
1-1.5 g/dL
46
Who gets leukoreduced packed cells?
1. Febrile transfustion reactions 2. Prevention of HLA antibodies 3. Oncology, Transplant, neonate patients
47
Who gets washed?
1. IgA deficient patients 2. EKMO patients/babies 3. Removal of anticoagulant or increased K+
48
Who gets Frozen/Deglycerolized RBCs
1. IgA deficient patients | 2. Rare phenotypes
49
Who gets CMV negative?
Bone Marrow Transplant patients Infant/neonatal patients Pregnant women
50
Random Platelets should increase the plt count ______ for each unit in pool
5-10,000/uL
51
Platelet Pheresis or "single -donor platelets" should increase the platelet count by _______
20-60,000/uL
52
Who gets HLA matched Aphaeresis Platelets?
Severely thrombocytopenic patient known to be refractory to random donors
53
Trigger for therapeutic platelet transfusion?
Less than 50,000/uL
54
Platelet transfusion trigger for patients with chemotherapy-induced thrombocytopenia
10-20,000/uL
55
What are 3 disease states when are platelets not needed?
ITP TTP HUS
56
Indications for Cyro
Fibrinogen less than or equal to 100 mg/dL Fibrinogen Less than 150 mg/dL with active hemorrhage - Treatment of Factor XIII deficiency - Fibrin Glue - Von Willebrand's disease

Blood Bank Rotation (6 decks)

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