BLOOD COMPONENT PREPARATION Flashcards
(38 cards)
Centrifugation must be done ____ hours after collection , to allow preparation of RBCs, platelet concentrates. frozen plasma and cryoprecipitate
8-24 hours
Heavy spin for PRBC, and Platelet concentrate:
5,000 x g for 5 minutes
Heavy spin for Cryoprecipitate:
5,000 x g for 7 minutes
Light spin for platelet-rich plasma (PRP)
2,000 x g for 3 minutes
Whole blood
Indications/uses:
Storage temp:
Shelf-life:
Volume:
Whole blood
Indications/uses:
- Symptomatic anemia with large-volume deficit
Storage temp: 1-6C
Shelf-life: depends on Ac
Volume: 450-500 mL
QC for whole blood:
Hct should be at 38-40%
Packed RBC
Indications/uses:
Storage temp:
Shelf-life:
Volume:
Packed RBC
Indications/uses:
- Symptomatic anemia but maybe at risk of circulatory overload
Storage temp: 1-6C
Shelf-life:
- Close System = depend on Ac (same as WB)
- Open System = 24 hours
Volume: 250-300 mL
QC for PRBC:
Hct must be 80% or less
RBC Aliquots
Indications/uses:
Storage temp:
Shelf-life:
Volume:
RBC Aliquots
Indications/uses: used in infants less than 4 months of age
Storage temp: 1-6C
Shelf-life: 24 hours
Volume: 10-25 mL/dose
Most common Ac used in RBC aliquots:
CPDA-1
QC for RBC aliquots:
Hct 80% or less
Leukocyte -Reduced RBC:
Indications/uses:
Storage temp:
Shelf-life:
Volume:
Leukocyte -Reduced RBC:
Indications/uses: prevents febrile reactions; reduces CMV and EBV transmission & HLA alloimmunization; p
Storage temp: 1-6C
Shelf-life:
- Close System = depend on Ac (same as WB)
- Open System = 24 hours
Volume: 250-300 mL
Washed RBCs
Indications/uses:
Storage temp:
Shelf-life:
Volume:
Washed RBCs
Indications/uses: Prevents allergic/anaphylactic reactions; removal of antibodies or other substances
Storage temp: 1-6C
Shelf-life: 24 hours
Volume: 180 mL
QC for Washed RBCs:
Hct: 70-80%
Dosage effect of WB, PRBC, Washed RBCs:
Elevated Hct 3% - 5%
Elevated Hb 1 - 1.5 g/dL
Frozen RBCs
Indications/uses:
Storage temp:
Shelf-life:
Volume:
Frozen RBCs
Indications/uses: For rare phenotyoes and autologous use
Storage temp/shelf life
- Frozen with glycerol: -65C = 10 years
- Deglycerolized units: 1-6C = 24 hours
Volume: 180 mL
Thawing of frozen RBCs:
Thawing is 45 minutes at 37C water bath and washed with decreasing saline concentration
How is platelet concentrate obtained?
Whole blood –> light spin –> PRP –>Heavy spin —> Platelet concentrate
Platelet content of random-donor platelet concentrate VS single-donor platelet concentrate:
Random-donor platelet concentrate: 5.5 x 10^10 platelets
Single-donor platelet concentrate: 3.0 x 10^11 platelets (equivalent to 4-6 random-donor platelets)
Plasma volume of random-donor platelet concentrate VS single-donor platelet concentrate:
Random-donor plt conc: 50-70 mL
Single-donor plt conc: 200-400 mL
Dosage effect of random-donor platelet concentrate VS single-donor platelet concentrate:
Random-donor plt conc:
elevates platelet by 5,000 - 10,000/uL
Single-donor plt conc:
elevates platelet by 30,000 - 60,000/uL
pH of Random and single-donor plt conc.;
pH 6.2 or higher
Shelf-life and storage temperature of random and single-donor plt conc:
5 days / 20-24C with constant agitation
Pooled units of Random-donor plt conc:
4 units
