blood+ hematopoiesis Flashcards
(17 cards)
what stage does nucleolus dissappear in erythropoiesis and granulopoiesis?
basophilic erythroblast
specific (neutrophilic) myelocyte
what is an important receptor in erythropoietin. Also what is final target of erythropoietin ?
JAK 2 kinase / janus kinase 2 which affects signaling pathway of erythropoietin
final target- DNA transcription
size of RBC and shape ?
7.5um and 2.6 um biconcave
only biconvex in reticulocyte stage
what is an important receptor in erythropoietin? Also, what is final target of erythropoietin ?
JAK 2 kinase / janus kinase 2 which affects signalling pathway of erythropoietin
final target- DNA transcription
pathological forms of rbc
macrocytosis and microcytosis
anisocytosis- both
poikilocytosis-deviation of shape
sickle cell
spherocytosis
acanthocytosis- projections of various sizes
key cytokines in first stage of granulocyte
cytokines are glycoproteins that stimulate proliferation of projenitor+ precursor cells
myeloblast has SCF (stem cell factor) and interleukins -3 (macrophage+monocyte, endothelia, CD4+ T-cells what HIV destroys- function to stimulate other immune cells as an immune response to pathogen
time for myeloblast to mature
neutrophilic myelocyte
10-14 days
1 week
lifespan of neutrophilic band
neutrophile
platelets
8-12h in blood and 1-2 days in CT
6-8h in blood and 1-4 days in CT
10 days
Platelet zones?
granule contents +size?
peripheral hyalumere and granulomere on central
Alpha granule 300-500nm: fibrinogen+factor V+VIII and platelet-derived growth factor
Delta granule e= dense, 250-300nm:
ADP/ATP+ serotonin and Ca
Lagma granule :
lysosomes w/ hydrolytic enzymes
azurophilic granules
oval nucleus w fine chromatin BASOPHILIC:
myeloperoxidase
lysosomes
defensins
neutrophilic granules
eisonophilic:
alkaline phosphatase
collagenase
lactoferrin
eisonophilic granules
12-14um eosinophilic w 1-2 weeks lifespan
MBP major basic proteins
acid phosphatase
eosinophil peroxidase
histaminase
collagenase
basophilic granules
10-12um basophilic+metachromatic like mast cells:
histamine+heparin
eosinophil chemotactic factor
platelet-activating factor
peroxidase
phosphatase A enzyme
number of leukocytes in blood?
150,000-400,000 platelets/uL
proteins associated w erythrocytes?
plasmolemma 40% lipids, 10% carbs and 50% proteins: integral proteins band 3 protein+ glycophorin A (has antigenic sites for ABO blood typing)
inner surface proteins: spectrin(make lattice that bound to actin F)+ akyrin (bnds lattice to integral proteins of erythrocyte - glycophorin A+ band 3 protein)
process of repair of tissue for leukocytes
1) cytokines- loosen intrajugular junction of endothelial cells in veins
2) P selectin (cell adhesion protein) it binds to glycosylated ligands on leukocytes surface (neutrophils)- interaction causes cells to slow down
3) diapedesis- leukocytes sends extensions to opening of endothelial cells (allows migration)
4) chemotaxis- aggregation of more leukocytes
platelets vs plasma cells structure
plasma cells are b lymphocyte-derived
antibody-producing cells
large, ovoid cells w spherical nucleus (w compact peripheral region w heterochromatin+ alter w light areas euchromatin=clockface
basophilic cytoplasm (rich RER)
golgi+centriole(pale when stained)
lifespan 10 days+abundant in infected cells
platelets:
structure: 10 days lifespan
2-4um, biconvex, small, non-nucleated+ membrane-bound in endothelial tissue
150,000-400,000/ uL
originate from end of cytoplasmic proc of megakaryocytes, promote clotting+ repair in endothelial,
discoid shape: peripheral hyalumere (light stained) and central granulomere (dark); Has marginal bundle: microtubule+microfilaments to maintain shape
