Blood Preparations

Question 1

anticoagulant used in hemostasis specimen collection

Answer 1

3.2% sodium citrate (blue top)

Question 2

3.2% sodium citrate (blue top) blood-anticoagulant ratio

Answer 2

9:1

Question 3

why EDTA is not used in hemostasis specimen collection?

Answer 3

EDTA irreversibly chelates calcium ions

Question 4

Maintaining the blood collection tube seal minimizes CO2 diffusion, which otherwise allows the pH to rise, falsely prolonging the _____ and _____

Answer 4

PT and PTT

Question 5

Specimens are maintained at ambient temperature, _____ to _____, never at refrigerator temperatures or on ice.

Answer 5

15C to 25C

Question 6

Storage at 1° C to 6° C activates ______, activates platelets, and precipitates large VWF multimers.

Answer 6

factor VII

Question 7

Specimens are never stored or transported at temperatures greater than 25° C because heat deteriorates coagulation factors __ and __

Answer 7

factors V and VIII

Question 8

The freezer tube is labeled, sealed, and frozen. It may be stored at ___ for up to 2 weeks or at ___ for long-term storage

Answer 8

-20C for up to 2 weeks
-70C for long term storage

Question 9

If it cannot be tested immediately, the specimen may be stored at _____ to _____ for up to ______ after thawing.

Answer 9

1C to 6C for up to 2 hours

Question 10

(T/F)
To avoid cryoprecipitation of VWF, specimens may not be frozen and thawed more than once.

Answer 10

true

Question 11

PRP

Answer 11

platelet-rich plasma

Question 12

PT

Answer 12

prothrombin time

Question 13

PTT

Answer 13

partial thromboplastin time

Question 14

UFH

Answer 14

unfractioned heparin

Question 15

It refers to the liquid part of an anticoagulated blood sample.

Answer 15

normal patient plasma (NPP)

Question 16

All clotting factors are still present such as fibrinogen.

Answer 16

normal patient plasma (NPP)

Question 17

It refers to a plasma sample (unclotted) left behind after an adsorption or simply the adherence of an antibody onto the surface of a red blood cell is done.

Answer 17

adsorbed plasma

Question 18

_____ adsorbed plasma provides

Answer 18

barium sulfate

Question 19

BARIUM SULFATE adsorbed plasma provides

Answer 19

fibrinogen
factor V, VIII, XI, XII, XIII

Question 20

While the vitamin K-dependent ______ are removed

Answer 20

Factors II, VII, IX, X

Question 21

It refers to an aged plasma sample (unclotted) deficient in Factor V & Factor VIII

Answer 21

aged normal plasma

Question 22

Not used immediately

Answer 22

aged normal plasma

Question 23

Used after 24 hours

Answer 23

aged normal plasma

Question 24

It is prepared by incubating normal serum for 24 hours at 37°C.

Answer 24

aged normal serum

Question 25

Unlike plasma, it does not contain clotting factors

Answer 25

aged normal serum

Question 26

It is a concentrated plasma rich in platelet obtained through a higher centrifugal speed of supernatant plasma.

Answer 26

platelet rich plasma

Question 27

Plasma with platelet count of 200,000/uL to 300,000/uL

Answer 27

platelet rich plasma

Question 28

Relative centrifugal force (RCF) of 50 x g for 30 minutes

Answer 28

platelet rich plasma

Question 29

PLATELET RICH PLASMA To produce sufficient PRP, the original specimen must measure ___ to ___ mL of whole blood.

Answer 29

9-12mL

Question 30

Are designed to test platelet rich plasma (PRP)

Answer 30

Light Transmittance Platelet Aggregometer (LTPA)

Question 31

It is the byproduct of PRP

Answer 31

platelet poor plasma

Question 32

It contains a very small amount of platelets.

Answer 32

platelet poor plasma

Question 33

Plasma with platelet count of <10,000/uL

Answer 33

platelet poor plasma

Question 34

Relative centrifugal force (RCF) of 1500 x g for 15 minutes

Answer 34

platelet poor plasma

Question 35

Used in CLOT-BASED PLASMA COAGULATION TEST

Answer 35

platelet poor plasma

Question 36

Plasma with platelet count of <5,000/uL

Answer 36

platelet free plasma

Question 37

Uses DOUBLE SPIN APPROACH

Answer 37

platelet free plasma

Question 38

Blood for whole blood platelet aggregometry, lumi-aggregometry. thromboelastography (TEG), or Rotational thromboelastometry (ROTEM) is collected with ______ and held at ___ to ___ C until testing.

Answer 38

3.2% sodium citrate 15C-25C

Question 39

(T/F) Chilling destroys platelet activity and is to be avoided.

Answer 39

true

Question 40

Most specimens for whole-blood aggregometry are mixed ____ with _______ before testing

Answer 40

1:1 with normal saline

Question 41

It is the most suitable blood preparation for detecting hypo-coagulable disorders specifically for prothrombin time (PT) test.

Answer 41

patient plasma

Question 42

When Prothrombin time (PT) or Activated Partial thromboplastin time (APTT) is performed on test plasma deficient of any of factor II, VII, IX, X, these tests show prolonged results. When these tests are repeated after mixing Adsorbed plasma with this test plasma, PT and APTT are still prolonged i.e. not corrected. If the test plasma is deficient in factors other than factor II, VII, IX, and X, on mixing with adsorbed plasma, it will replenish those factors from adsorbed plasma, thus the tests will be said to be corrected.

Answer 42

adsorbed normal plasma

Question 43

It is used in mixing studies; it involves combining the test plasma with normal plasma, then repeating the screening test on the mixture to assess whether the clotting time becomes normal or remains prolonged.

Answer 43

aged normal plasma

Question 44

It is used to correct deficiency which has a normal PT, abnormal APTT and is not corrected by adsorbed plasma, Factor IX.

Answer 44

aged normal serum

Question 45

it is used as control in coagulation studies such as APTT and PT

Answer 45

platelet-rich plasma

Question 46

It prevents pre-analytical errors in preparing since platelets can operate as a phospholipid surface for clotting factor activation, interfering with laboratory diagnosis.

Answer 46

platelet-poor plasma