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Microbiology Finals > Brucella > Flashcards

Brucella Flashcards

(70 cards)

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1
Q

Haemophilus influenze
Classification

Based on capsular antigen

A

Serotype

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2
Q

Haemophilus influenze
Classification

Based on chemical properties

A

Biotype

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3
Q

Haemophilus influenze
Classification

Useful for clinical purposes

A

Biogroup

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4
Q

Haemophilus influenze
Culture

Flat grayish brown colonies after 24 hours incubation

A

Chocolate agar

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5
Q

Haemophilus influenze
Culture

Does not grow on

A

Sheep blood agar

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6
Q

Haemophilus influenze

Requires

A 
X factor (Hemin)
V factor (NAD)
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7
Q

Table of characteristics of growth and requirements

A

Memorize

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8
Q

Haemophilus influenze
Virulence factor

Anti phagocytic
Main virulence factor
Type b

A

Capsule

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9
Q

Haemophilus influenze
Virulence factor

Lipooligosaccharide saccharides (endotoxin)

A

Somatic antigen

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10
Q

Haemophilus influenze
Type B

Most common serotype causing systemic disease

A 
Meningitis
Pneumonia and emphysema
Epiglottitis
Cellulitis
Septic arthritis
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11
Q 
Haemophilus influenze
Non typable (non encapsulated)
A 
Sinusitis
Opportunistic
Chronic bronchitis
Conjuctivitis
Otitis media
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12
Q

Haemophilus influenze

Secondary to bacteremic spread from nasopharynx

A

Meningitis

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13
Q

Haemophilus influenze

Reddish blue patches on cheeks or peri orbital areas

A

Cellulitis

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14
Q

Haemophilus influenze

Children

A

Arthritis

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15
Q

Haemophilus influenze

Epidemic or endemic
H. Influenza bio group aegypticus

A

Conjunctivitis

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16
Q

Haemophilus influenze

Prevention

A

Chemoprophylaxis with Rifampicin

Hib conjugate vaccine

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17
Q

Haemophilus aegypticus

A

Knock weeks bacillus

Resembles H. Influenza closely

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18
Q

Haemophilus aegypticus

Diseases

A

Conjuctivitis

Brazilian purpuric fever

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19
Q

Chancroid
Ulcer on genitalia
Painful and enlarged lymph nodes

A

Hemophilus ducreyi

Best on chocolate agar

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20
Q

Bordatella pertusis

A 
Gram negative
Coco bacillary 
Encapsulated
Non motile
Strict aerobes
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21
Q

Bordatella pertusis

With bipolar metachromatic granules

A

Toluidine blue stain

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22
Q

Bordatella pertusis

Form acid from

A

Glucose

Lactose

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23
Q

Bordatella pertusis

Culture

A

Bordet gengou medium
Potato blood glycerol agar
Contain Pen G.

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24
Q

Bordatella pertusis

Virulence genes

A

bvgA and

bvgA

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25
Bordatella pertusis Virulence factors Protein on pili
Filamentous hemagglutinin
26
Bordatella pertusis Virulence factors Lymphocyte do not enter lymphoid tissue Sensitization to histamine Enhanced insulin secretion
Pertusis toxin
27
Bordatella pertusis Virulence factors Inhibit phagocytosis, increase cAMP
Adenyl cyclase toxin
28
Bordatella pertusis Adheres to and multiplies rapidly on epithelial surface of
Trachea | Bronchi
29
Bordatella pertusis MOT
Airborne droplets
30
Bordatella pertusis Source of infection
Catarrhal stage
31
Bordatella pertusis Disease
Pertusis or whooping cough
32
Bordatella pertusis Incubation pwriod
2 weeks
33
Bordatella pertusis | Stages
Catarrhal- highly infectious but not very ill Paroxysmal - hacking coughs, "whoops" Convalescence- slow
34
Bordatella pertusis Diagnosis Specimen
Saline nasal wash (preferred) | Nasopharyngeal swab
35
Bordatella pertusis Diagnosis 50% sensitivity
Direct fluorescence antibody test
36
Bordatella pertusis Diagnosis Most sensitive
PCR
37
Bordatella pertusis Diagnosis On 3 rd week of illness
Serology
38
Bordatella pertusis Chemoprophylaxis
Erythromycin
39
Bordatella pertusis | Vaccines
Acellular vaccine - inactivated pertusis vaccine | DPT x 3 doses
40
Brucella
``` Zoonotic Gram negative Coccobacilliary Aerobic Non motile Non spore forming ```
41
Brucella Positive in
Catalase Oxidase Urease
42
Brucella Culture
Soy agar | Blood culture media
43
Brucella Virulence factor Anti phagocytosis
Fusion of lysosomal granules
44
Brucella Virulence factor Sugar found in animal placenta
Tropism for erythritol
45
Brucella | MOT
Ingestion Droplets Skin contact Inhalation
46
Brucella Species of brucellosis
Melitensis- goats - acute and sever infection Suis - swine - suppurative lesions, caseating granuloma Abortus - cattle - no suppurative lesions, non caseating granuloma Canis - dogs - mild disease
47
Brucella Clinical brucellosis ( undulant or Malta fever)
Acute- influenza like syndrome | Chronic - non bacteremic, psychoneurotic
48
Brucella Diagnosis Serology
Increase IgM during 1st week of illness, peak at 3 months
49
Brucella | Treatment
Tetracycline+streptomycin | Trimethoprim - sulfamethazole
50
Pasteurella
Non motile | Aerobes or facultative aerobes
51
Pasteurella Postive
Oxidase | Catalase
52
Pasteurella Most common organism in human wounds inflicted by bites from cats and dogs
Pasteurella multocida
53
Pasteurella multocida Treatment
Pen G doc | Tetracyclines and Fluoroquinolones alternatives
54
Francisella Tularensis Culture
Small colonies within 1-3 days in Cysteine blood agar or Glucose blood agar
55
Francisella Tularensis Mot
Skin abrasion (most common) Bite of arthropod Direct contact with infected animal tissue Inhalation of aerosol Ingestion of contaminated food or water
56
Francisella Tularensis Tularemia Incubation period
3-5 days
57
Francisella Tularensis Tularemia Sign and symptoms
Ulceroglandular- ulcers at site of infection | Oculoglandular- direct eye inoculation
58
Francisella Tularensis Tularemia Treatment
Live attenuated vaccine | Streptomycin
59
Yersinia
Bipolar staining Gram negative Microaerophillic or facultative anerobic
60
Yersinia Positive
Positive catalase | Negative oxidase
61
Yersinia Virulence factor Anti phagocytic
Fraction 1
62
Yersinia Virulence factor Essential for virulence
72- kb plasmid
63
Yersinia | MOT
Bite of rat flea | Inhalation of infective droplets
64
Yersinia pestis Clinical manifestations
Bubonic plague- inflamed enlarged LN | Pneumonic - sepsis, death
65
Yersinia pestis Diagnosis
Smears- giemsa, fluorescent, wayson's stain Culture- blood agar, mocConkey's Serology
66
Yersinia pestis Treatment
Streptomycin doc | Tetracycline alternative
67
Y. Enterocolitica
Contaminated food Fomites Abdominal pain, diarrhea Mimic appendicitis
68
Yersinia pseudo tuberculosis
Fecal oral route | Caseous nodules in the payers patches and mesenteric nodules
69
Yersinia enterocolitica and pseudo tuberculosis | Treatment
3rd generation Cephalosporin and aminoglycosides Fluoroquinolones and another anti microbial Resistant to ampicillin and 1stgen
70
Heamophillus
Grams negative Facultative anaerobes Obligate parasites

Microbiology Finals (1 decks)

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