Brucellaceae

Question 1

Name selective staining method for Brucella

Answer 1

Stamp (positive = red, negative = green)

Question 2

What type of pathogen are Brucella?

Answer 2

Obligate pathogens - cause zoonoses

Question 3

Microscopical examination of Brucellaceae

Answer 3

Gram -
Small rods or coccobacilli
No specific arrangement
Non-motile
Acid-resistant

Question 4

Brucella relation to oxygen

Answer 4

aerobic or microaerophilic

Question 5

Brucella reaction on catalase, oxidase and urease

Answer 5

Catalase +, oxidase + and urease +

Question 6

Brucella growth on common nutrient media

Answer 6

Weak

Question 7

What kind of parasites are Brucellaea?

Answer 7

Intracellular parasites, able to multiply within the macrophages

Question 8

Brucellaceae affinity to reproductive tract

Answer 8

Cause placentitis, abortus, infertility, orchitis and epididymitis

Question 9

Virulence factors of Brucellaceae

Answer 9

Endotoxin
Somatic O-antigen
Intracellular parasitism

Question 10

Important zoonotic species of Brucellaceae (5)

Answer 10

Brucella abortus
Brucella melitensis
Brucella suis
Brucella canis
Brucella neotomae

Question 11

Bovine brucellosis is caused by:

Answer 11

Brucella abortus

Question 12

Ovine and caprine brucellosis is caused by

Answer 12

Brucella melitensis

Question 13

Brucellosis of swine and hares is caused by

Answer 13

Brucella suis

Question 14

Canine brucellosis is caused by

Answer 14

Brucella canis

Question 15

Brucellosis in rodents is caused by

Answer 15

Brucella neotomae

Question 16

Which Brucella is NOT a zoonotic pathogen?

Answer 16

Brucella Ovis

Question 17

Brucella Ovis causes

Answer 17

infectious epididymitis of rams

Question 18

How is Brucella excreted?

Answer 18

In bovine milk

Question 19

Material for cultivation of Brucellaceae

Answer 19

fetus, lavage from uterus, vaginal swabs, lymph nodes, blood or milk

Question 20

Cultivation properties of Brucellaceae

Answer 20

S (or R) form
Aerobic, microaerophilic (5-10% CO2)
37 degrees Celsius (20-40C)
Incubation time is 3-5 days

Question 21

Cultivation media for Brucella

Answer 21

enriched media such as blood serum, tissue extract, vitamins or 1% glycerin

Question 22

Brucella on blood agar

Answer 22

Small
Shiny
Transparent colonies
Blue sheen
No haemolysis
S or R form

Question 23

What are serological tests for Brucella and Myobacterium based on?

Answer 23

Detection of postinfection antibodies
Required during transport of animals
For prevention

Question 24

What antigen does Brucella and Myobacterium have?

Answer 24

Somatic O antigen: A, M and R. Part of lipopolysaccharide-protein complex.

Question 25

Name serological tests for blood

Answer 25

Agglutination (slide, tube) Complement Fixation Test ELISA

Question 26

Serological test for milk

Answer 26

Milk ring test (slow tube agglutination)

Question 27

Special test for Brucella (alergenodiagnostics)

Answer 27

Dermal test - application of F-allergen (abortin or brucelin) which causes inflammation and induration in 24-72 hours. Used for alergenodiagnostics - late hypersensitivity reaction