BUN

Question 1

is the principle waste product of protein catabolism.

Answer 1

Urea

Question 2

It is synthesized in the liver from __________ which is produced as a result of the deamination of amino acids.

Answer 2

ammonia

Question 3

It is synthesized in the liver from ammonia which is produced as a result of the _____________________.

Answer 3

deamination of amino acids

Question 4

Normally, urea nitrogen in the blood comprises only about _______ of the non-protein nitrogen.

Answer 4

45%

Question 5

The importance of urca nitrogen detenni-nation is its value as an indicator of ________ and ________ functions.

Answer 5

liver and kidney

Question 6

Decreases in Blood Urea Nitrogen (BUN) are seen with

Answer 6

nephritis
acute liver destruction
amyloidosis
pregnancy.

Question 7

Increases in BUN are encountered with

Answer 7

acute and chronic nephritis
intestinal and urinary obstruction
uremia
metallic poisoning
pneumonia
Addison’s Disease
peritonitis
surgical shock
cardiac failure.

Question 8

The Stanbio BUN procedure is a modification of the method described by

Answer 8

Sampson

Question 9

is catalytically converted to ammonium carbonate by the use of urcase.

Answer 9

Urea

Question 10

Urea is catalytically converted to ammonium carbonate by the use of ____

Answer 10

urease

Question 11

The reaction rate is dependent upon the concentration of the influence of _________________

Answer 11

glutamic dehydrogenase

Question 12

can be measured by the rate of conversion of NADH to NAD by the change of absorbancy at _______nm.

Answer 12

340 nm

Question 13

Two enzyme in urea

Answer 13

Urease
Glutamic dehydrogenase

Question 14

BUN Buffer

Answer 14

Tris
2-oxoglutarate
Monosodium salt
Urease
Glutamic dehydrogenase

Question 15

BUN ENZYME COMPOSITION

Answer 15

Disodium salt

Question 16

Reagent preparation: Before use allow to stand for at least ____ minutes at room tempsrature (15-25°C)

Answer 16

30 minutes

Question 17

Reagent preparation: Before use allow to stand for at least 30 minutes at _____________

Answer 17

room tempsrature (15-25°C)

Question 18

Reagent Storage and Stability: when properly stered at

Answer 18

2-8C

Question 19

Reagent Storage and Stability: stable for 4 weeks at

Answer 19

2-8°C

Question 20

Reagent Storage and Stability: 5 days

Answer 20

RT (15-25C)

Question 21

Preferred specimen of choice

Answer 21

Non-hemolyzed serum

Question 22

Anticoagulant to avoid

Answer 22

Ammonium
Fluoride salt

Question 23

serum: stable for up to 24 hours at

Answer 23

room temperature (15-25°C).

Question 24

Serum: several days refrigerated at

Answer 24

2-8°C

Question 25

Serum: at least 2-3 months when

Answer 25

frozen (-20°C).

Question 26

No interference

Answer 26

Bilirubin (40mg/dl) Hemoglobin (200mg/dl) Triglycerides (2000mg/dl)

Question 27

Working reagent (ml/ul)

Answer 27

1.0ml (1000ul)

Question 28

Warm at

Answer 28

37C for 3 mins

Question 29

Add ________ serum to unknown cuvette and mix

Answer 29

0.01ml (10 ul)

Question 30

After exactly _________ read und record absorbance

Answer 30

30 seconds

Question 31

After exactly 30 seconds read und record absorbance of

Answer 31

A1

Question 32

Al exactly______ seconds after reacing (A,), read and record absorbance

Answer 32

60 sec

Question 33

Al exactly 60 seconds after reacing (A,), read and record absorbance

Answer 33

A2

Question 34

Calculate change in absorbance

Answer 34

A1-A2 = Au

Question 35

Add _______ standard to control cuvette

Answer 35

0.01ml (10ul)

Question 36

Serum computations

Answer 36

Au/A(s/c) X 30

Question 37

Expected value: bun

Answer 37

8-23 mg/dl

Question 38

Expected value: urea

Answer 38

17-49 mg/dl

Question 39

Urea: mg/dl =

Answer 39

bun x 2.14

Question 40

Urea : mmol/L =

Answer 40

Urea (mg/dl) x 0.167

Question 41

Principle of BUN

Answer 41

Protein —> ammonia —> urea (non protein nitrogen) Urea—> h2o —> (urease) 2NHy+ 2HO3 2- oxoglutarate + NHy+ Disodium salt Glutamic dehydrogenase—> L- glutamate + NAD + H2O