calcium aa Flashcards
(6 cards)
why do we externally calibrate for calcium concentration
external standards are created with known concentrations to calibrate the atomic absorption spectrophotometer, allowing for the determination of calcium concentration in unknown seawate
what is the beer lambert law
The Beer-Lambert Law describes how absorbance is related to concentration, where absorbance depends on the molar absorption coefficient, path length, and concentration. it helps us determine concentration based on the intensity of light
why do we use flame atomic absorption spectroscopy
Flame atomic absorption spectrophotometry (AA) is used to measure the absorbance of light by atomized analytes in a flame, where the sample is aspirated, evaporated, and atomized at high temperatures. The instrument measures the intensity of light that passes through the flame, which is related to the concentration of the analyte.
why do we do a di blank and a reagent blank
A DI blank (deionized water blank) is used to account for any background absorbance or interference from the water itself, ensuring that any readings are solely due to the analyte in the sample. It helps ensure the accuracy of measurements by correcting for the contribution of the solvent.
A reagent blank is used to account for any absorbance or interference from the chemicals or reagents used in the experiment, ensuring that the instrument response is only due to the analyte of interest and not due to impurities or reactions from the reagents.
what do calibration curves with linear regression tell us in this context
In this context, a calibration curve with linear regression helps establish a relationship between the known concentrations of the calcium standard solutions and their corresponding absorbance values measured by the atomic absorption spectrophotometer.
