Cancer and the Immune System Flashcards
(32 cards)
The role of the immune system is what?
Maintain tissue homeostasis
What resides within all healthy tissue ?
Many immunocytes particularly t cells along with sentinel myeloid dendritic cells and macrophages. Their role is to be conscious to damage to the tissue either through infection with bacteria, viruses but also in terms of transformation.
Explain the schreiber immunosurveillance immunoediting model of tumourigenesis?
Normal cells subject to common oncogenic stimuli ultimately undergo transformation and become tumour cells
At early stages of tumourigenesis, these cells may express distinct tumour-specific markers and generate pro-inflammatory “danger” signals that initiate the cancer immunoediting process
Cancer immunoediting is an extrinsic tumor suppressor mechanism that engages only after cellular transformation has occurred and intrinsic tumor suppressor mechanisms have failed. In its most complex form, cancer immunoediting consists of three sequential phases: elimination, equilibrium, and escape.
Elimination: innate and adaptive immunity work together to destroy developing tumors long before they become clinically apparent.If this phase goes to completion, then the host remains free of cancer, and elimination thus represents the full extent of the process.
If, however, a rare cancer cell variant is not destroyed in the elimination phase, it may then enter the equilibrium
Equilibrium: Tumour outgrowth is prevented by immunologic mechanisms. T cells, IL-12, and IFN-γ are required to maintain tumor cells in a state of functional dormancy, whereas NK cells and molecules that participate in the recognition or effector function of cells of innate immunity are not required; this indicates that equilibrium is a function of adaptive immunity only. Editing of tumor immunogenicity occurs in the equilibrium phase. Equilibrium may also represent an end stage of the cancer immunoediting process and may restrain outgrowth of occult cancers for the lifetime of the host. However, as a consequence of constant immune selection pressure placed on genetically unstable tumor cells held in equilibrium, tumor cell variants may emerge that (i) are no longer recognized by adaptive immunity (antigen loss variants or tumors cells that develop defects in antigen processing or presentation), (ii) become insensitive to immune effector mechanisms, or (iii) induce an immunosuppressive state within the tumor microenvironment. These tumor cells may then enter the escape phase, in which their outgrowth is no longer blocked by immunity. These tumor cells emerge to cause clinically apparent disease.
What is the immunotherapy cure rate for metastatic melonoma?
50%
How is the immune system classified?
Humoral and cellular
Further divided into innate and adaptive
Innate: “Hard coded”, No memory and rapid
Adaptive: Somatic recombination, memory, can take time
Humoral Innate: C reactive protein, Complement Cytokines
Humoral Adaptive: Antibodies, Cytokines
Cellular innate: Neutrophils, Eosinophills, Basophils, Monocyte/macrophages, dendritic cells and natural killer cells
Cellular adaptive:
B cells, Alpha beta T cells (CD4 & CD8 T cells)
What bridges humoral innate and adaptive immunity?
Natural antibodies
What bridges cellular innate and adaptive immunity?
NK T cells
yo T cells
What are T Cells
Adaptive immune cells
Capacity to see virtually any organism
Use the T cell receptor (TCR) to see things
See things presented to them on MHC
CD8 T cells can directly kill cells that do not look healthy (e.g. virus infected, cancerous)
Explain the major histocompatibility complex class 1
Self protein synthesis
Some proteins degraded by proteosome, this chops protein to short fragments which then are loaded onto surface of MHC class 1 molecule
Which is then exported onto surface of cells and provides immune system ability to look into healthy of this cell.
“Endogenous” peptide allows CD8 T cells to see what’s going
on inside
Explain the major histocompatibility complex class 1 when a virus is present
Viruses are obligate intracellular parasites because they absolutely depend on host cell for protein translation as do not have any organelles.
Virus enters nucleus, transcribes into mRNA and this leads to viral protein synthesis.
Most of viral protein will be incorporated into viral membrane, some however will be degraded by proteosome.
In virally infected cell, the cell is aware there is a viral infection and is able to change this proteosome to a stress proteosome and this produces more peptides.
Peptides that are chopped are then loaded onto MHC class 1 which is exported onto surface of cell and is now presenting a foreign peptide. If CD8 is activated to see foreign peptide, is then aware that this exists and then will kill this cell.
Explain the major histocompatibility complex in cancer
In cancer mutant/ abnormal protein typically arise, some of which are degraded by proteosome and loaded on MHC class 1 and others are not.
MHC class 1 alerts CD8 cell which then kills cell
T cell receptor diversity exceeds the human genome coding capacity
How is this possible?
To generate diversity, RAG enzyme is expressed
RAG- Recombination activated gene
RAG leads to somatic recombination of each Variable, Diversity, Junction gene from B chain locus and this somatic combination attains combinatorial diversity. This will not attain 10 to the 8 diversity
This diversity achieved because as part of RAG complex one of the enzymes within complex Terminal deoxynucleotidyl transferase(TdT), transfers extra nucleotides into junctions between V, D and J and this leads to junctional diversity, this process is random (junctions can be very long or short). This results in random diversity of T cells and thus may generate T cell receptors that are entirely ineffective or some that react again self which leads to autoimmunity and ultimately tissue damage- opposite of role of immune system.
Development within the thymus deletes many but not all- thymus important for tolerising against cells and developing t cells but this process is not 100%
RAG is only switched on in what?
Thymus: T cells
Bone marrow: B cells
What is peripheral intolerance?
What does this highlight?
Process whereby a mature naïve T cell that only receives one signal via T cell receptor becomes anergic. Important as if this t cell were to observe an antigen, the most likely antigen it will observe is self antigen, likely a healthy one and so the safest response is to become anergic.
Immune system is built to tolerate self peptides.
Mature naïve T cell encounters a dendritic cells (professional antigen presenting cells, role is to present antigens to T cells)
Here dendritic cells in tissue has transported bacteria in tissue, digests bacteria, and presents bacterial peptide on MHC
In this case here T cell is a perfect match for bacterial antigen in the context of MHC with this T cell receptor
What happens to CD8 T cell?
A naïve T cell that only receives TCR signal (signal 1) becomes anergic
Important as within gut billions of bacteria exist which may be pathogenic, however in gut are perfectly symbiotic and responding against them would lead to colitis
Dendritic cell resides within tissues that display tissue damage – inflammation and inflammatory cytokines.
Stressed cells present which may be releasing extra signals such damage associated molecular patterns.
Inflammation, stressed cells, damage associated molecular patterns inform dendritic cell that tissue in which it resides in is not healthy and that it is inflamed.
Dendritic cell then transfers damage to MHC and signals to T cell that the tissue is unhealthy.
Thus now bacterial antigen is presented with a second signal of danger/inflammation/unhealthy tissue.
Second signal is CD80 and CD86, once both presented they engage a molecule on the t cell called cd28 which delivers a second signal to the t cell
What happens to CD8 T cell?
A naïve T cell that receives TCR signal (signal 1) AND ”co- stimulation” (signal 2) becomes activated and becomes a “licensed” memory cell (no longer naïve)
Once a naive t cell becomes a licensed memory t cell what occurs?
Co stimulation is not obligatory to kill infected/cancer cell
How is autoimmunity prevented
If have a stressed cell for example after an infection/chemical damage/transformation, the stressed cell may be releasing its own peptides and these peptides will be presented on MHC.
Thus now self peptide presented in context of MHC along with a second signal here. Danger here is activation of self reactive CD8 t cells.
Many autoimmune diseases that are clinically relevant are probably triggered by initial infectious or inflammatory insults within the tissues
There is a further, potent regulation of this process to try and prevent autoimmunity – presence of T suppressor t regulatory cells (Treg), this recognises self peptide but it expresses on it several inhibitory molecules –CTLA4 and this molecule binds CD80 and CD86 with a much higher affinity than CD28 and this means if there is a Treg present and this is specific to the self peptide then this will sequester this signal 2 and remove signal 2 from CD8 t cells. Thus one signal left and t cell is anergised.
T cell also excrete large amount of interleukin 10, ( immunosuppressive cytokine that prevents activation and proliferation of other T cells)
Finally T-regs are full of interleukin 2 receptors and thereby it acts as a IL-2 sink and all of this occurs locally to suppress any t cells sat around this dendritic cell expressing self peptide
What do all t cells need to group and therefore is expressed by all t cells?
Cytokine interleukin 2
Stressed cell (red) e.g through prolonged inflammation, bystander damage, metabolic cost or generally bad
If CD8 t cell activated and specific for this cell will secrete cytokines and try to kill stressed cell
Issue is that cytokines are not hugely specific, most will be focused on this cell but some of them will leak and affect surrounding healthy cells
The purpose of the immune system is to maintain tissue homeostasis – thus do want to rid of stressed cell but do not want to kill other normal cells
How can this be overcome?
Thus part of an inbuilt physiological mechanism to protect tissues against excessive CDH killing or excessive inflammation several mechanisms happen.
- Cytokines that the CD8 t cell secrete also activate negative regulator of CD8 t cells, thus all cd8 t cells will express receptor PD1 and in epithelial tissues when CD8 t cells activated is that a negative feedback mechanism (upregulation of PDL1) occurs. Binding of PDL1 to pd1 suppresses CD8 cell, makes it less likely to secrete cytokines and less likely to divide
- Other mechanism is several tregs arise and suppress t cells and this is a physiological mechanism to prevent excessive inflammation within tissues
Stressed cell (red) e.g through prolonged inflammation, bystander damage, metabolic cost or generally bad
If CD8 t cell activated and specific for this cell will secrete cytokines and try to kill stressed cell
Issue is that cytokines are not hugely specific, most will be focused on this cell but some of them will leak and affect surrounding healthy cells
The purpose of the immune system is to maintain tissue homeostasis – thus do want to rid of stressed cell but do not want to kill other normal cells
How can this be overcome?
Thus part of an inbuilt physiological mechanism to protect tissues against excessive CDH killing or excessive inflammation several mechanisms happen.
- Cytokines that the CD8 t cell secrete also activate negative regulator of CD8 t cells, thus all cd8 t cells will express receptor PD1 and in epithelial tissues when CD8 t cells activated is that a negative feedback mechanism (upregulation of PDL1) occurs. Binding of PDL1 to pd1 suppresses CD8 cell, makes it less likely to secrete cytokines and less likely to divide
- Other mechanism is several CTLA-4 + tregs arise and suppress t cells and this is a physiological mechanism to prevent excessive inflammation within tissues
PD-L1 and CTLA-4 + Treg infiltration are PHYSIOLOGICAL responses to inflammation to limit excessive tissue damage (not cancer specific)
What checkpoints exist in the physiological setting?
CTLA-4 is important peripheral tolerance mechanism
PD1/PDL1 axis is important in protecting tissues from excessive inflammation
Self proteins are more likely to what? tolerize immune system
Tolerize immune system
Likelihood of responding to antigens is dependent upon how similar they are to self, thus if very similar to healthy human self, peripheral tolerance mechanism is much more likely to suppress any immune response generated
Whereas if different i.e. from a different species, more likely to elicit immune response as tregs will not be specific to staphylococcus antigen for instance
Issue is that cancer is mutant self and closer to healthy human cell
How can this be overcome?
However now understand how immune checkpoint works, can try to block it, in particular CTLA4 which inhibits CD80 and CD86 simulation and can use drug or antibody (Iipulimubab) which binds to CTLA4 and prevents association with CD80 and CD86 thus restoring co-stimulation to t cells which may be searching for a peptide.
