Carbohydrates Flashcards

(42 cards)

1
Q

Possible Samples for Carbohydrates

A

Whole blood
Plasma
Serum
CSF
Pleural Fluid
Urine

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2
Q

Carbohydrate sample not used for Diabetic Diagnosis

A

Urine

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3
Q

How long can serum be stable at 25 deg C

A

8hrs

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4
Q

How long can serum be stable at 4 deg C

A

72 hrs

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5
Q

2 types of Glucose Methodologies

A

Chemical Method
Enzymatic Method

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6
Q

2 Types of Chemical Method

A

Oxidation Reduction Method
Condensation Method

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7
Q

Reduction of cupric ions to cuprous ions forming cuprous oxide in hot alkaline solution by glucose

A

Alkaline Copper Reduction Method

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8
Q

4 methods in Alkaline Copper Reduction Method

A

Folin Wu
Nelson Somogyi
Neocuproine
Benedict’s

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9
Q

Stabilizing agent for Benedict’s method

A

Citrate or Tartate

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10
Q

Involves reduction of a yellow ferricyanide to a colorless ferrocyanide by glucose

A

Hagedom Jensen

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11
Q

2 types Oxidative Reduction Method

A

Alkaline Copper Reduction Method
Alkaline Ferric Reduction Method

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12
Q

Under Condensation Method

A

Dubowski method

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13
Q

Dubowski Method will yield a what colored compound

A

green

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14
Q

2 types of glucose oxidase method

A

Colorimetric Glucose Oxidase
Polarographic Glucose Oxidase

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15
Q

Most specific Glucose Method

A

Hexokinase Method

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16
Q

Dextrostics are also called

A

Cellular Strip

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17
Q

Random Plasma Glucose was formerly known as

A

Random Blood Sugar

18
Q

Fasting Plasma Glucose was formerly known as

A

Fasting Blood Sugar

19
Q

Fasting Plasma Glucose specimens are collected after when

A

8-10hrs fasting

20
Q

When is fasting plasma glucose specimen ideally collected

21
Q

Specimens for Oral Glucose Tolerance Test

A

Urine and Blood

22
Q

OGTT fasting time

23
Q

Different types of loads for OGTT

A
  1. 50grams (base line & 1st hour) (2x collection)
  2. 75 grams (base line up to 2nd hr) (3x collection
  3. 100grams (base line up to 3rd hr) (4x collection)
24
Q

HbA1c is also called as

A

Glycated Hemoglobin

25
Normal value for Glycated Hemoglobin
4-6%
26
Specimen Requirement for Glycated Hemoglobin
Serum Plasma Whole blood
27
Anticoagulant of choice of HbA1c
Citrate Heparin Sodium Fluoride EDTA
28
Most widely used to assess short term glycemic control
Fructosamine
29
What does fructosamine measure
Glucose that binds to the protein
30
A test to detect very small levels of protein (albumin) in urine
Urine Microalbumin
31
Test to detect Type 1 Diabetes Mellitus
Ketone Testing
32
Ketone Testing Methods
Gerhardt's Sodium Nitroprusside 3-hydroxybutyrate dehydrogenase
33
Diagnostic Criteria for Diabetes Mellitus: Random Plasma Glucose level
greater than 200mg/dL
34
Diagnostic Criteria for Diabetes Mellitus: Fasting Plasma Glucose level
greater than 126mg/dL
35
Diagnostic Criteria for Diabetes Mellitus: 2 hour Plasma Glucose OGTT level
greater than 200mg/dL
36
Diagnostic Criteria for Diabetes Mellitus: Gycated Hemoglobin level
greater than 6.5%
37
Alkaline Copper Reductive: Cuprous Ions + Phosphomolybdate
Folin Wu method
38
Alkaline Copper Reductive: Cuprous Ions + Arsenomolybdate
Nelson Somogyi
39
7 Laboratory Tests for Glucose
Random Plasma Glucose Fasting Plasma Glucose Tolerance Test HbA1c Fructosamine Urine Microalbumin Ketone Testing
40
5 Enzymatic Methods
Glucose Oxidase Method Hexokinase Method Glucose Dehydrogenase Method Dextrostics Interstitial Glucose Measuring Device
41
Cuprous Ions + Phosphomolybdate -> ?
Phosphomolybdic Acid
42
Cuprous Ions + Neocuproine -> ?
Cuprous-Neocuproine Complex