Carbohydrates Flashcards
(42 cards)
Possible Samples for Carbohydrates
Whole blood
Plasma
Serum
CSF
Pleural Fluid
Urine
Carbohydrate sample not used for Diabetic Diagnosis
Urine
How long can serum be stable at 25 deg C
8hrs
How long can serum be stable at 4 deg C
72 hrs
2 types of Glucose Methodologies
Chemical Method
Enzymatic Method
2 Types of Chemical Method
Oxidation Reduction Method
Condensation Method
Reduction of cupric ions to cuprous ions forming cuprous oxide in hot alkaline solution by glucose
Alkaline Copper Reduction Method
4 methods in Alkaline Copper Reduction Method
Folin Wu
Nelson Somogyi
Neocuproine
Benedict’s
Stabilizing agent for Benedict’s method
Citrate or Tartate
Involves reduction of a yellow ferricyanide to a colorless ferrocyanide by glucose
Hagedom Jensen
2 types Oxidative Reduction Method
Alkaline Copper Reduction Method
Alkaline Ferric Reduction Method
Under Condensation Method
Dubowski method
Dubowski Method will yield a what colored compound
green
2 types of glucose oxidase method
Colorimetric Glucose Oxidase
Polarographic Glucose Oxidase
Most specific Glucose Method
Hexokinase Method
Dextrostics are also called
Cellular Strip
Random Plasma Glucose was formerly known as
Random Blood Sugar
Fasting Plasma Glucose was formerly known as
Fasting Blood Sugar
Fasting Plasma Glucose specimens are collected after when
8-10hrs fasting
When is fasting plasma glucose specimen ideally collected
Morning
Specimens for Oral Glucose Tolerance Test
Urine and Blood
OGTT fasting time
8-10hrs
Different types of loads for OGTT
- 50grams (base line & 1st hour) (2x collection)
- 75 grams (base line up to 2nd hr) (3x collection
- 100grams (base line up to 3rd hr) (4x collection)
HbA1c is also called as
Glycated Hemoglobin