Carbohydrates & Amyloid

Question 1

What control is used for PAS stain?

Answer 1

kidney

Question 2

What is the purpose of the PAS reaction?

Answer 2

The demonstration of polysaccharides, neutral mucosubstances, and basement membranes.

Question 3

WhaRedt is another name for the Schiff reagent?

Answer 3

leucofuchsin

Question 4

What reagents on used for the PAS?

Answer 4

Periodic acid, HCL, Potassium metabisulfite, Schiff reagent

Question 5

What is the oxidizer for the PAS?

Answer 5

Periodic acid

Question 6

What is the purpose of the Schiff reagent?

Answer 6

Reduction causes the loss of the quinoid structure and masking of the chromphores.

Question 7

What do potassium metabisulfite in the PAS reaction?

Answer 7

Remove excess Schiff reagent and prevent false colorization of tissue elements because of oxidation of any adsorbed reagent.

Question 8

What do you use to check if the Schiff reagent is fresh?

Answer 8

Formaldehyde

Question 9

What color will the reaction turn if the Schiff reagent is fresh?

Answer 9

reddish purple

Question 10

What color will the reaction turn if the Schiff reagent is bad?

Answer 10

deep blue-purple

Question 11

How must Schiff reagent be stored?

Answer 11

In the Fridge

Question 12

What color is the PAS reaction

Answer 12

Bright rose

Question 13

What control is used for PAS diatase digestion?

Answer 13

liver containing glycogen or cervix

Question 14

What is the purpose of PAS diastase digestion?

Answer 14

Demonstration of glycogen in tissue

Question 15

What color will glycogen stain

Answer 15

Bright rose

Question 16

What do the Best carmine stain demonstrate?

Answer 16

glycogen

Question 17

Which technique for glycogen is more specific PAS or Best Carmine?

Answer 17

PAS

Question 18

What is the preferred fixative for PAS?

Answer 18

10% NBF or Bouin

Question 19

Fixative for PAS diatase digestion?

Answer 19

10% NBF, formalin alcohol, or absolute alcohol

Question 20

Fixative for best carmine

Answer 20

Absolute alcohol, Carnoy and Bouin can also be used

Question 21

Control for best carmine

Answer 21

liver

Question 22

reagents used for best carmine

Answer 22

Carmine stock solution, Working carmine solution, differentiating solution

Question 23

What is the counterstain for both PAS reactions?

Answer 23

Harris hematoxylin

Question 24

What is Harris hematoxylin used for in the best carmine stain?

Answer 24

To stain the nuclei

Question 25

What color is glycogen and Nuclei stained in best carmine?

Answer 25

Glycogen-Pink to red | Nuclei- Blue

Question 26

What is the purpose of Mayer mucicarmine?

Answer 26

Staining of epithelial mucin in tissue

Question 27

What is the fixative for Mayer mucicarmine?

Answer 27

10% NBF

Question 28

Quality control for Mayer mucicarmine

Answer 28

unautolyzed colon, small intestine, or appendix

Question 29

Reagents used for Mayer mucicarmine

Answer 29

Working mucicarmine solution, Weigert Iron hematoxylin, Mentanil yellow

Question 30

What is the nuclear stain in the Mayer mucicarmine stain?

Answer 30

Weigert iron hematoxylin

Question 31

What is the counter stain in the Mayer mucicarmine stain?

Answer 31

Mentanil yellow

Question 32

Results of Mayer mucicarmine

Answer 32

Mucin-Deep rose to red Capsule of cryptococcus- Deep rose to red Nuclei- Black Other tissue elements- Blue to yellow

Question 33

How can the carminophilic properties be obscured

Answer 33

Overstaining with either hematoxylin or metanil yellow

Question 34

What will happen is Gill hematoxylin is used for the nuclear stain of Mayer mucicarmine?

Answer 34

The mucin may have a bluish cast

Question 35

Why can you not use autolyzed tissue as a control for Mayer mucicarmine stain?

Answer 35

Goblet cells can not be positively identified

Question 36

What is the purpose of Alcian blue, pH 2.5?

Answer 36

Demonstration of acid mucopolysaccharides

Question 37

What element do Alcian blue get it color from?

Answer 37

copper

Question 38

Fixative for Alcian Blue

Answer 38

10% NBF or Bouin

Question 39

Quality control for Alcian blue 2.5 and 1.0

Answer 39

unautolyzed small intestine, appendix, or colon

Question 40

Reagent used for Alcian blue 2.5

Answer 40

Acetic acid 3%, Alcian blue 1%, Nuclear fast red (kernechtrot)

Question 41

What stain can be used for identification of adenocarcinomas?

Answer 41

Mayer mucicarmine

Question 42

What is the counter stain for Alcian blue?

Answer 42

Nuclear fast red

Question 43

Results of alcian blue 2.5

Answer 43

Weakly acidic sulfated mucosubstances, hyaluronic acid and sialomucins- Dark blue Background-Pink to red Nuclei- Red

Question 44

Purpose of alcian blue 1.0

Answer 44

stain sulfated mucosubstances

Question 45

Reagents of alcian blue 1.0

Answer 45

0.1 N HCL, 1% alcian blue, Nuclear fast red

Question 46

Results of alcian blue 1.0

Answer 46

sulfated mucosubstances- Pale blue Background- pink to red Nuclei- red

Question 47

What is the purpose of Alcian blue-PAS hematoxylin reaction

Answer 47

Differentiate between neutral and acidic mucosubstances; used in many laboratories today for the detection of intestinal metaplasia.

Question 48

What is the principle of Alcian blue-PAS-hematoxylin?

Answer 48

Acidic mucosubstances are stained with the alcian blue technique and neutral mucosubstances are stained by the PAS reaction

Question 49

Fixative for Alcian blue-PAS-hematoxylin

Answer 49

10% NBF or Zenker