CBS blood donor products

Question 1

2 canadian systems for blood

Answer 1

hema quebec and Canadian blood services replaced the red cross bc of the krever inquiry

Question 2

how frequently can men & women donate blood

volume and time

Answer 2

56 days for men ( 7 x yr)

84 days for women ( 4 x yr)
- women have less iron so less donations

480mL collected in 10-15 mins

Question 3

apheresis platelets and plasma can be donated every

Answer 3

14 days for plts
7 days for plasma

Question 4

donor eligibility

Answer 4

17 yrs old
at least 110lbs
hgb(g/L) 125(F) 130 (M)

Question 5

temporary defferal from donating

Answer 5

tattoos, peircings- 3 months

travel risk for malaria or zika

aspirin- affects plt function ( 72 hrs)

Question 6

permenant deferral from donating

Answer 6

taking money or drugs in exchange for sex since 1977 or using IV street drugs

lived in an area with vCJD outbreak

Question 7

mandatory donor testing

Answer 7

ABO/ Rh and other antigens

antibody screen

serological/ NAT testing for
- HIV 1& 2
- hep B & C
- HTLV
- WNV ( june- nov)
- syphilis ( treponema pallidum )

Question 8

additional testing

Answer 8

chagas ( treonema cruzi)- if donors mother or grandmother resided in central/south america

out of season WNV - travel risk

CMV- for fetal intrauterine transfusion

Question 9

testing for plts

Answer 9

plts are stored in warm temps ( more prone to bacterial growth)
therefore they are tested 36 hrs after collection and are in culture bottles for up to 7 days

Question 10

plasma testing

Answer 10

pooled plasma for products such as IVIG are tested for parvovirus B19
B19 causes fifth disease

Question 11

anticoagulants in blood and purpose of each

Answer 11

480mL of blood collected with 70 mL of CPD ( citrate, phosphate, dextrose)

sodium citrate: binds calcium ( prevents coagulation )

citric acid: creates acidic environment to slow down glycolysis

monobasic sodium phosphate: buffer to maintain pH

dextrose: provides source of sugar for cell metabolism

Question 12

2 methods for separating whole blood

Answer 12

1. buffy coat method ( B1)
   2.whole blood filtration ( B2)

Question 13

buffy coat method(B1)

Answer 13

separated into:

blood ( filtered)

plasma unit

buffy coat( 4 buffy coat donors and 1 plasma unit for pooled plt unit)

Question 14

whole blood method ( B2)

Answer 14

filtered first then separated into :

rbc unit

plasma unit ( frozen )
- frozen plasma
- exported for further manufacturing
- first step in cryo

Question 15

packed RBC unit after processing
- volume
- storage additive

Answer 15

~290mL
- SAGM ( saline-adenine-glucose-mannitol)

Question 16

purpose of SAGM

Answer 16

prevents storage lesions
- maintains ATP and 2-3 DPG to help with oxygen levels and reduce potassium leakage from inside cells

Question 17

storage for RBC units and shelf life

Answer 17

stored 1-6 degrees or 1-10 if in transit for less than 24hrs

shelf life 42 days

Question 18

timeline to transfuse RBCs

Answer 18

once breeched must be transfused in 24hrs if refrigerated or within 4 hrs if above 6 degrees

if removed from temp control and not transfused must be returned within 1 hr

Question 19

frozen RBC units

Answer 19

frozen with glycerol for up to 10 years below -65 degrees ( done for rare phenotypes)

before transfusion RBCs thawed and washed to deglycerolize; must be transfused in 24hrs

Question 20

disoloration acceptable for transfusion

Answer 20

plasma discoloration
-hemolysis up to 0.8%
- icterus
-green ( oral contraceptives
-orange ( vit A/ carrots )
- lipemia

Question 21

how to tell if bacterial contamination is in RBCs

Answer 21

excessive air bubbles
dark purple or black

• NOT accceptable

Question 22

acceptable particulate matter

Answer 22

white particulate matter - lipid material that may dissipate and change with temp

Question 23

NOT acceptable particulate matter

Answer 23

particulates that dont dissipate with gentle manipulation
- clots/ fibrin strands
- cellular aggregates
- cold agglutinin masses

Question 24

storage and shelf life of plts

Answer 24

stored at 20- 24 degrees under continuous agitation in gas permeable containers ( CO2 from metabolic activity casues pH to drop)

shelf life : 7 days

Question 25

when would plts be irradiated

Answer 25

for immunocompromised patients

Question 26

apheresis fresh frozen plasma

Answer 26

collected by plasmaphersis and frozen within 8 hrs

Question 27

frozen plasma

Answer 27

collected from RBC unit frozen within 24hrs

Question 28

plasma storage

Answer 28

shelf life of 12 months -18 degrees

once defrosted dont refreeze

Question 29

cryo preparation

Answer 29

cryo is frozen plasma defrosted separated and refozen as cryosupernatant and cryoprecipitate

Question 30

solvent detergent plasma prep, shelf life, and when to use

Answer 30

plasma pooled from many donors and tested

shelf life 4 years

certain conditions - TTP & HUS

Question 31

directed donations

Answer 31

collected from a parent or guardian and given to their minor child

this has increased risk to the patient

Question 32

Autologous donations

Answer 32

preoperative patient donating blood weeks prior to surgery for self transfusion
- ONLY cases where whole blood is transfused

may be necessary for extremely rare blood types however usually causes pre- operative anemia ( counter productive)

Question 33

stem cells

Answer 33

CBS has a stem cell registry searching for compatible stem cells ( cord blood) & bone marrow donors