CC Week 5 - Instrumentation

Question 1

The measurement of light scattered by a

particulate solution

Answer 1

NEPHELOMETRY

Question 2

scattered light is measured at an

Answer 2

angle to the incident light when small particles

are involved

Question 3

for large molecules

Answer 3

forward light

scatter can be measured.

Question 4

True or False:
The amount of scatter is directly proportional
to the number and size of particles present in the
solution

Answer 4

true

Question 5

Measures light blocked as a decrease in the
light transmitted through the solution, dependent
on particle size and concentration

Answer 5

TURBIMETRY

Question 6

Uses a spectrophotometer for measurement

Answer 6

TURBIMETRY

Question 7

The problems inherent in turbidimetry

Answer 7

Variation in particle size of samples
- Variation in particle size of standards
- Rate of aggregation or settling of
particles

Question 8

Type of luminescence where excitation requires

absorption of radiant energy

Answer 8

MOLECULAR EMISSION SPECTROSCOPY

Question 9

emission of light by a
substance that has absorbed light or other
electromagnetic radiation

Answer 9

Fluorescence

Question 10

emitted light
has a longer wavelength, and therefore,
lower energy than the absorbed radiation

Answer 10

form of luminescence

Question 11

measurement of the

emitted fluorescence light

Answer 11

Fluorometry

Question 12

Fluorometers use

Answer 12

filters

Question 13

spectrofluorometers use

Answer 13

prisms or

diffraction gratings as monochromators

Question 14

True or false 
Primary advantage of performing 
fluorometric over absorption 
spectrometric methods of analysis is 
increased specificity and increased 
sensitivity.

Answer 14

True

Question 15

emission of light
produced by certain substances after they
absorb energy

Answer 15

emission of light
produced by certain substances after they
absorb energy

Question 16

Types of luminescence where excitation does

not require absorption of radiant energy.

Answer 16

(a) Chemiluminescence

(b) Bioluminescence

Question 17

chemical energy of
a reaction produces excited atoms and
upon electron return to ground state,
photons of light are emitted

Answer 17

Chemiluminescence

Question 18

is the substance used to

generate light signal

Answer 18

Ruthenium

Question 19

enzyme-catalyzed

chemical reaction produces light emission

Answer 19

Bioluminescence

Question 20

is a generic term for the
type of instrument that is used to
measure chemiluminescence and
bioluminescence.

Answer 20

Luminometer

Question 21

Technique where solutes in a sample are
separated for identification based on physical
differences that allow their differential
distribution between a mobile phase and a
stationary phase.

Answer 21

CHROMATOGRAPHY

Question 22

Mobile phase may be an

Answer 22

inert gas or liquid

Question 23

Stationary phase may be

Answer 23

Silica glass

Question 24

Type of planar chromatography

Answer 24

Thin Layer Chromatography

Question 25

Commonly used detectors include

Answer 25

flame ionization, thermal conductivity, electron capture and mass spectrometer.

Question 26

is a type of liquid chromatography where the mobile phase is a liquid that is passed over the stationary phase of the column.

Answer 26

HPLC

Question 27

strength of solvent | remains constant during separation

Answer 27

Isocratic elution

Question 28

strength of solvent continually increases (%/min) during separation.

Answer 28

Gradient elution

Question 29

polar stationary phase and nonpolar | mobile phase

Answer 29

Normal-phase liquid chromatography