Cell Biology Flashcards
(230 cards)
What are microscopes used for?
To see objects that cannot be seen with the naked eye
When did purposeful magnification first appear?
Before 2500 BCE
In which century were lenses first invented?
12-13th century
Fill in the blank: Microscopes are instruments that are used to see objects that cannot be seen with the _______.
naked eye
True or False: Microscopes have been in use since before 500 CE.
True
Who are credited with creating the forerunner of the compound microscope?
Zaccharias Janssen and Hans Janssen
They experimented with multiple lenses placed in a tube.
In what year did Hooke publish his book Micrographia?
1665
This book described his observations made with a microscope.
What did Hooke observe through the microscope that led him to name them ‘cells’?
Pores/compartments in cork
His observations were made on slivers of cork.
Who is known as the ‘Father of Microbiology’?
Van Leeuwenhoek
He built numerous simple microscopes and discovered many microorganisms.
What type of microscopes did Van Leeuwenhoek build?
Simple microscopes with single lenses
He achieved magnifications up to 270X.
What did Van Leeuwenhoek study using his microscopes?
Bacteria, yeast, plants, and small organisms in pond water
His studies included a variety of small life forms.
Fill in the blank: The early compound microscope was developed in _______.
1595
This development involved the use of multiple lenses.
True or False: Hooke was the first to observe cells.
True
Hooke’s observations in cork led to the naming of cells.
Who first used specific dyes to stain specific structures in cells?
Joseph von Gerlach
He did this in 1858 and recognized the importance of staining.
What significant contribution did Oskar Heimstädt make in 1911?
Constructed the first successful fluorescence microscope
This microscope uses a high-intensity light source to excite fluorescent molecules.
What is the principle behind fluorescence microscopy?
It uses a high-intensity light source to excite a fluorescent molecule, which emits lower energy light of a longer wavelength
This emitted light produces the magnified image.
In what year was the first electron microscope invented?
1931
It was co-invented by German scientists Max Knott and Ernst Ruska.
What does an electron microscope use to illuminate a specimen?
An electron beam
This allows it to produce a magnified image.
What are the two main types of electron microscopes?
- TEM (transmission electron microscopes)
- SEM (scanning electron microscopes)
Each type has distinct imaging capabilities.
What type of images do TEM microscopes obtain?
2D images
They can magnify up to ~1,000,000X.
What type of images do SEM microscopes obtain?
3D images of surface structures
They can magnify up to ~100,000X.
Fill in the blank: The first successful fluorescence microscope was constructed by _______.
Oskar Heimstädt
Who first used specific dyes to stain specific structures in cells?
Joseph von Gerlach
He did this in 1858 and recognized the importance of staining.
What significant contribution did Oskar Heimstädt make in 1911?
Constructed the first successful fluorescence microscope
This microscope uses a high-intensity light source to excite fluorescent molecules.